Methamphetamine (MA) use disorder is a new health problem among Iranian female methadone patients that needs special treatment and rehabilitation services. However, few describe their baseline characteristics, their reasons for MA use while in treatment, and their treatment needs and success. The study aimed at investigating these research gaps. Methods: The participants were 70 women with MA use disorder in two large women-only methadone treatment services in Tehran, Iran. A researcher-made checklist, the Severity of Dependence Scale, the Contemplation Ladder, and the General Health Questionnaire-28 were completed by women in a baseline interview. Results: The Mean±SD age of the participants was 35.84±8.62 years. The participants reported high severity of MA dependence (Mean±SD = 6.88±3.19), poor motivation to change (Mean±SD = 4.0±2.31]), and impaired psychological well-being (Mean±SD = 16.35±4.04) in the treatment. The most important reasons for MA use while undergoing methadone treatment included self-treatment (55%) and pleasure-seeking (43%). The most important MA treatment needs included effective psychological treatments (75%), continued family support (72%), and life skills (68%). Stable living and financial conditions (90%), drug-free living and working places (86%), continued family support (78%), and psychological counseling (69%) were the most important reported factors associated with MA treatment success. Discussion: The current study indicated that participants reported a number of MA-related problems. This issue needed a comprehensive treatment program at individual, social, psychological, and professional levels. The findings have important implications for delivering psychosocial treatments, as well as gender-specific research.