Burden of different beta-lactamase classes among clinical isolates of AmpC-producing <i>Pseudomonas aeruginosa</i> in burn patients: A prospective study

Kumar, Vinod; Sen, Malay Ranjan; Nigam, Chaitanya; Gahlot, Rupam; Kumari, Shweta

doi:10.4103/0972-5229.102077

Cited by 21 publications

(19 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different rates of inducible AmpC producer P. aeruginosa strains were recorded in previous studies, varying from 30% [19] and up to 60% [20]. Being difficult to detect without being alert to its presence, inducible AmpC producers have to be searched for in routine laboratories whenever P. aeruginosa is isolated, especially with the increasing rates of ceftazidime resistance.…”

Section: Discussionmentioning

confidence: 99%

Lab Based Surveillance of Multidrug Resistant Pseudomonas aeruginosa in Cairo University Hospitals, Egypt

Zafer¹

2015

JMEN

View full text Add to dashboard Cite

Multidrug resistance is increasingly recorded among P. aeruginosa causing health care associated infections (HAI) due to the selective pressure by over-usage of antibiotics. The primary aim of the study was to perform a laboratory based surveillance to investigate the prevalence of β-lactamase and AmpC production and multi-drug resistance among clinical isolates of P. aeruginosa causing health care associated infections in Kasr Al Aini School of Medicine and National Cancer Institute, Cairo University, Egypt. β-lactamase and AmpC production was tested by the double disc test. Multidrug-resistant P. aeruginosa (MDRPA) were defined as demonstrating resistance to carbapenems, all anti-pseudomonal cephalosporins, amino glycosides and fluoroquinolones. In a six months period, out of 12,524 clinical samples, 2593 (20.7%) P. aeruginosa isolates were identified. Out of these isolates, 1138 (43.9%) were ceftazidime resistant, 138 (5.3%) were ESBL producers, and 661 (25.5%) isolates were confirmed positive for inducible AmpC beta-lactamase. The proportion of multidrug-resistant P. aeruginosa was 12.3%. In conclusion, high prevalence of AmpC indicates the necessity of performing routine tests to detect AmpC in ceftazidime resistant P. aeruginosa isolates. Lab based surveillance is important to identify the prevalence of MDRPA in order to guide empirical antibiotic therapy and prevent the spread of resistant strains.

show abstract

Section: Discussionmentioning

confidence: 99%

Lab Based Surveillance of Multidrug Resistant Pseudomonas aeruginosa in Cairo University Hospitals, Egypt

Zafer¹

2015

JMEN

View full text Add to dashboard Cite

show abstract

“…In Mediterranean countries, such as Italy and Greece, VIM-positive P. aeruginosa strains of clonal complex 235 and 111 have been involved in nosocomial outbreaks [38]. The activity of aztreonam, as the only β-lactam not hydrolyzed by MBLs, is strongly compromised by the copresence of ESBLs, plasmid-mediated AmpC, or other serine β-lactamase together with MBLs [49,50]. Bla VIM genes are typically embedded in class 1 integrons, which can be incorporated in either plasmids or chromosome.…”

Section: Class B Carbapenemasesmentioning

confidence: 99%

Carbapenemases

Bedenić¹,

Sardelić²

2019

Growing and Handling of Bacterial Cultures

View full text Add to dashboard Cite

Carbapenems are usually regarded as the last treatment option for serious infections caused by Gram-negative and Gram-positive microorganisms. Although they are stable to hydrolysis by most β-lactamases, their usage as the last resort antbiotics was seriously compromised by the appearance of carbapenemhydrolyzing enzymes called carbapenemases. These enzymes are produced mostly by Enterobacteriaceae and Gram-negative nonfermentative bacteria such as Pseudomonas aeruginosa and Acinetobacter baumannii. True carbapenemases belonging to Ambler molecular classes A, B, and D are often encoded by genes embedded in mobile genetic elements like plasmids, integrons, and transposons, which often harbor multiple resistance determinants limiting further the treatment options. At present, large number of nosocomial and community-acquired infections caused by worldwide spread of carbapenem-resistant Gram-negative bacteria have become a major public health problem. Although polymyxins remain active, in vitro reports of benefits of combination schemes favor this strategy against carbapenemaseproducing Gram-negative bacilli.

show abstract

“…Therefore, early identification of the infections due to these microorganisms is necessary as the appropriate treatment might reduce the spread of these resistant strains as well as the mortality in hospitalized patients. This emphasizes the need for the detection of isolates that produce these enzymes to avoid therapeutic failures and nosocomial outbreaks in hospitals (Kumar et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

AmpC and metallo beta-lactamases producing Gram negative bacteria in patients with hematological malignancy

El-Kazzaz¹,

El-Khier²

2015

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Resistance to broad spectrum β-lactams mediated by AmpC and metallo beta-lactamases (MBLs) enzymes is a rising problem worldwide. The wide dissemination of Gram negative bacteria harboring these enzymes represents a significant clinical threat during the last decade, which is mainly due to treatment failure and restriction of therapeutic options. This problem should be really estimated in our locality with special emphasis on immunocompromised patients. The aim of this study was to isolate Gram negative bacteria from differrent sites of infection among patients with hematological malignancy, and to examine those isolates for AmpC and MBLs production by phenotypic and genotypic methods. Seventy four Gram negative bacterial strains were isolated from 387 clinical samples collected from different infection sites. Those isolates were screened for the presence of AmpC and MBLs by modified three dimensional test and Imipenem-EDTA combined disc test, respectively. Multiplex PCR was done as a confirmatory step for detection of AmpC and MBLs production by these isolates. Pseudomonas aeroginosa was the most common isolated Gram negative strain that was found to be positive for AmpC and MBL production. DHA gene was the most frequently detected AmpC β-lactamase gene, whereas VIM was the only detected MBL gene among the Gram negative bacterial isolates by multiplex PCR. The strong association found between AmpC production and MBL gene carriage is alarming which necessitate continuous surveillance of such resistance mechanisms among the Gram negative bacteria, especially in patients with hematological malignancy.

show abstract

Burden of different beta-lactamase classes among clinical isolates of AmpC-producing Pseudomonas aeruginosa in burn patients: A prospective study

Cited by 21 publications

References 25 publications

Lab Based Surveillance of Multidrug Resistant Pseudomonas aeruginosa in Cairo University Hospitals, Egypt

Lab Based Surveillance of Multidrug Resistant Pseudomonas aeruginosa in Cairo University Hospitals, Egypt

Carbapenemases

AmpC and metallo beta-lactamases producing Gram negative bacteria in patients with hematological malignancy

Contact Info

Product

Resources

About