Buried Food-seeking Test for the Assessment of Olfactory Detection in Mice

Machado, Cleiton F.; Reis-Silva, Thiago Marinho; Lyra, Cassandra Santantonio de; Felício, Luciano Freitas; Malnic, Bettina

doi:10.21769/bioprotoc.2468

Cited by 18 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To evaluate the impact of prolonged passive olfactory exposure on different aspects of olfaction, we initially performed the buried food test (Machado et al, 2018; Rattazzi et al, 2015; Yang & Crawley, 2009) that measures the animal's ability to locate a piece of food and its innate tendency towards foraging (Machado et al, 2018; Rattazzi et al, 2015; Yang & Crawley, 2009). We found that the latency to find the hidden food was similar between prolonged passive olfactory‐exposed animals (36.17 ± 11.70 s; n = 6; Figure 7a) and control non‐implanted animals (34.28 ± 11.83 s; n = 7; p > 0.05; Figure 7a).…”

Section: Resultsmentioning

confidence: 99%

Main olfactory bulb reconfiguration by prolonged passive olfactory experience correlates with increased brain‐derived neurotrophic factor and improved innate olfaction

Hernández-Soto

Pimentel-Farfan

Elva

et al. 2022

Eur J of Neuroscience

View full text Add to dashboard Cite

The main olfactory bulb (MOB) is highly plastic and constantly reconfiguring its function and structure depending on sensory experience. Despite the extensive evidence of anatomical, functional and behavioural changes in the olfactory system induced by highly variable olfactory experiences, it is still unknown whether prolonged passive odour experience could reconfigure the MOB at its input and network activity levels and whether these changes impact innate olfaction. Here, by measuring odour-induced glomerular activation, MOB network activity and innate olfactory behaviours, we described a profound MOB reconfiguration induced by prolonged passive olfactory experience in adult animals that impacts MOB input integration at the glomerular layer including an increase in the activated glomerular area and signal intensity, which is combined with a refinement in the number of activated glomeruli and less-overlapped glomerular maps. We also found that prolonged passive olfactory experience dramatically changes MOB population activity in the presence and absence of odours, which is reflected as a decrease in slow oscillations (<12 Hz) and an increase in fast oscillations (>12 Hz). All these functional changes in awake and anaesthetized mice correlate with an increase in brain-derived neurotrophic factor (BDNF) and with improved innate olfactory responses such as habituation/dishabituation and innate preference/avoidance. Our study shows that prolonged passive olfactory experience in adult animals produces a dramatic reconfiguration of the MOB network, possibly driven by BDNF, that improves innate olfactory responses.

show abstract

Section: Resultsmentioning

confidence: 99%

Main olfactory bulb reconfiguration by prolonged passive olfactory experience correlates with increased brain‐derived neurotrophic factor and improved innate olfaction

Hernández-Soto

Pimentel-Farfan

Elva

et al. 2022

Eur J of Neuroscience

View full text Add to dashboard Cite

show abstract

“…The BFT was conducted to evaluate consciousness, attention, motivation, and organised thinking [42]. The procedure was carried out as described in the literature with some modifications [96]. Briefly, the rat was supplied one piece of sweetened cereal two days before the test.…”

Section: Buried Food Testmentioning

confidence: 99%

Lipopolysaccharide-Induced Delirium-like Behaviour in a Rat Model of Chronic Cerebral Hypoperfusion Is Associated with Increased Indoleamine 2,3-Dioxygenase Expression and Endotoxin Tolerance

Ang,

Makpol,

Nasaruddin

et al. 2023

IJMS

View full text Add to dashboard Cite

Indoleamine 2,3-dioxygenase (IDO) and the tryptophan–kynurenine pathway (TRP-KP) are upregulated in ageing and could be implicated in the pathogenesis of delirium. This study evaluated the role of IDO/KP in lipopolysaccharide (LPS)-induced delirium in an animal model of chronic cerebral hypoperfusion (CCH), a proposed model for delirium. CCH was induced by a permanent bilateral common carotid artery ligation (BCCAL) in Sprague Dawley rats to trigger chronic neuroinflammation-induced neurodegeneration. Eight weeks after permanent BCCAL, the rats were treated with a single systemic LPS. The rats were divided into three groups: (1) post-BCCAL rats treated with intraperitoneal (i.p.) saline, (2) post-BCCAL rats treated with i.p. LPS 100 μg/kg, and (3) sham-operated rats treated with i.p. LPS 100 μg/kg. Each group consisted of 10 male rats. To elucidate the LPS-induced delirium-like behaviour, natural and learned behaviour changes were assessed by a buried food test (BFT), open field test (OFT), and Y-maze test at 0, 24-, 48-, and 72 h after LPS treatment. Serum was collected after each session of behavioural assessment. The rats were euthanised after the last serum collection, and the hippocampi and cerebral cortex were collected. The TRP-KP neuroactive metabolites were measured in both serum and brain tissues using ELISA. Our data show that LPS treatment in CCH rats was associated with acute, transient, and fluctuated deficits in natural and learned behaviour, consistent with features of delirium. These behaviour deficits were mild compared to the sham-operated rats, which exhibited robust behaviour impairments. Additionally, heightened hippocampal IDO expression in the LPS-treated CCH rats was associated with reduced serum KP activity together with a decrease in the hippocampal quinolinic acid (QA) expression compared to the sham-operated rats, suggested for the presence of endotoxin tolerance through the immunomodulatory activity of IDO in the brain. These data provide new insight into the underlying mechanisms of delirium, and future studies should further explore the role of IDO modulation and its therapeutic potential in delirium.

show abstract

“…To detect behavioral olfactory deficits, we conducted two standardized buried food tests to optimize the detection of any anomalies in olfactory function in the early vs. late stages (46)(47)(48). Both tests determine the ability of mice to detect a food reward hidden in the bedding of clean housing cages.…”

Section: Buried Food Testsmentioning

confidence: 99%

α-Synuclein pathology and reduced neurogenesis in the olfactory system affect olfaction in a mouse model of Parkinson’s disease

Martín-López

Greer

Chandra

et al. 2022

Preprint

View full text Add to dashboard Cite

Parkinson Disease (PD) is characterized by multiple symptoms including olfactory dysfunction, whose underlying mechanisms remain unclear. Here, we explored pathological changes in the olfactory pathway of transgenic (Tg) mice expressing the human A30P mutant alpha-synuclein (alpha-syn) (alpha-syn-Tg mice) at 6-7 and 12-14 months of age, representing early and late-stages of motor progression, respectively. alpha-Syn-Tg mice at late stages exhibited olfactory behavioral deficits, which correlated with severe alpha-syn pathology in projection neurons of the olfactory pathway. In parallel, olfactory bulb (OB) neurogenesis in alpha-syn-Tg mice was reduced in the OB granule cells at 6-7 months, and OB periglomerular cells at 12-14 months, respectively, both of which could contribute to olfactory dysfunction. Proteomic analyses showed a disruption in endo- and exocytic pathways in the OB during the early stages which appeared exacerbated at the synaptic terminals when the mice developed olfactory deficits at 12-14 months. Our data suggest that, 1) the alpha-syn-Tg mice recapitulate the olfactory functional deficits seen in PD; 2) olfactory structures exhibit spatiotemporal disparities for vulnerability to alpha-syn pathology; 3) alpha-syn pathology is restricted to projection neurons in the olfactory pathway; 4) neurogenesis in adult alpha-syn-Tg mice is reduced in the OB; and 5) synaptic endo- and exocytosis defects in the OB may further explain olfactory deficits.

show abstract

Buried Food-seeking Test for the Assessment of Olfactory Detection in Mice

Cited by 18 publications

References 12 publications

Main olfactory bulb reconfiguration by prolonged passive olfactory experience correlates with increased brain‐derived neurotrophic factor and improved innate olfaction

Main olfactory bulb reconfiguration by prolonged passive olfactory experience correlates with increased brain‐derived neurotrophic factor and improved innate olfaction

Lipopolysaccharide-Induced Delirium-like Behaviour in a Rat Model of Chronic Cerebral Hypoperfusion Is Associated with Increased Indoleamine 2,3-Dioxygenase Expression and Endotoxin Tolerance

α-Synuclein pathology and reduced neurogenesis in the olfactory system affect olfaction in a mouse model of Parkinson’s disease

Contact Info

Product

Resources

About