Burkholderia cepacia Complex Regulation of Virulence Gene Expression: A Review

Sousa, Sílvia A.; Feliciano, Joana R.; Pita, Tiago; Guerreiro, Soraia I.; Leitão, Jorge H.

doi:10.3390/genes8010043

Cited by 38 publications

(33 citation statements)

References 103 publications

(173 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although Burkholderia has been previously demonstrated to be a member of vaginal communities, the prevalence has been underappreciated, and its role in the community is unclear. It should be noted that Burkholderia is intrinsically resistant to clinically relevant antibiotics, which might confer a positive effect on the subversion of immune functions and the elicitation of inflammation, thus potentially facilitating the occurrence of SIL.…”

Section: Discussionmentioning

confidence: 99%

Cervicovaginal microbiota composition correlates with the acquisition of high‐risk human papillomavirus types

Huang

et al. 2018

Intl Journal of Cancer

View full text Add to dashboard Cite

High-risk (hr) human papillomavirus (HPV) infection is closely associated with the clinical conditions of both squamous intraepithelial lesions (SILs) and cervical carcinoma. However, it remains unclear what factors determine the type of hrHPV infection. Here, we have comprehensively investigated the bacterial composition of the cervicovaginal microbiota of 280 women infected with one type of hrHPV (HPV 16, 52 or 58) by the pyrosequencing of barcoded 16S rRNA genes. Differential microbiota composition was observed among various SIL groups and within the subgroups of each group. This result showed that it is not the microbiota diversity or the common microbiota, but rather agents that are specific to each SIL that might have a positive influence on the acquisition of hrHPV types, independent of abundance. Specifically, a composition of Oribacterium, Lachnobacterium and Thermus in the cervicovaginal microbiota is more likely to be associated with HPV 16, while a composition of Motilibacter in the cervicovaginal microbiota is more likely to be associated with HPV 52, and a composition of Litorilinea and Paludibaculum with a concomitant paucity of L. iners in the cervicovaginal microbiota is more likely to be associated with HPV 58. Furthermore, functional predictions regarding infectious diseases and cancer-related genes disclosed significant differences (p < 0.01) among the different (sub)groups. Our study provides an elucidation of the relationship between the composition of the cervicovaginal microbiota and the type of hrHPV acquired.

show abstract

Section: Discussionmentioning

confidence: 99%

Cervicovaginal microbiota composition correlates with the acquisition of high‐risk human papillomavirus types

Huang

et al. 2018

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…Bacteria of the Burkholderia cepacia complex (BCC) are known opportunistic pathogens causing a plethora of infections and clinical complications, resulting in significant morbidity and mortality, especially in the hospitalized immunocompromised population and outbreak and intensive care settings (Sousa et al, 2017;Gautam et al, 2011;Mangram and Jarvis, 1996;Mahenthiralingam et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Epidemiological investigation and successful management of a Burkholderia cepacia outbreak in a neurotrauma intensive care unit

Rastogi

Khurana

Veeraraghavan

et al. 2019

International Journal of Infectious Diseases

View full text Add to dashboard Cite

Objective: The detailed epidemiological and molecular characterization of an outbreak of Burkholderia cepacia at a neurotrauma intensive care unit of a level 1 trauma centre is described. The stringent infection control interventions taken to successfully curb this outbreak are emphasized. Methods: The clinical and microbiological data for those patients who had more than one blood culture that grew B. cepacia were reviewed. Bacterial identification and antimicrobial susceptibility testing was done using automated Vitek 2 systems. Prospective surveillance, environmental sampling, and multilocus sequence typing (MLST) were performed for extensive source tracking. Intensive infection control measures were taken to further control the hospital spread. Results: Out of a total 48 patients with B. cepacia bacteraemia, 15 (31%) had central line-associated blood stream infections. Two hundred and thirty-one environmental samples were collected and screened, and only two water samples grew B. cepacia with similar phenotypic characteristics. The clinical strains characterized by MLST typing were clonal. However, isolates from the water represented a novel strain type (ST-1289). Intensive terminal cleaning, disinfection of the water supply, and the augmentation of infection control activities were done to curb the outbreak. A subsequent reduction in bacteraemia cases was observed. Conclusion: Early diagnosis and appropriate therapy, along with the rigorous implementation of essential hospital infection control practices is required for successful containment of this pathogen and to curb such an outbreak.

show abstract

“…Mex1 has a unique sequence type (ST), the ST 423, but genetically it is closely related to internationally spread clones as it belongs to the clonal complex 31, the largest clonal complex of all STs reported in the Multi-Locus Sequence Typing (MLST) B. cepacia complex database [ 16 ]. Until now, the adaptation process of B. cenocepacia to the host environment has been investigated for clinical isolates only [ 40 ] and, despite the description of several virulent factors, understanding is still poor [ 41 ]. Considering environmental B. cenocepacia strains, increased fitness of the B. cenocepacia HI2424 strain, belonging to the recA lineage IIIB, has been shown suggesting the adaptation of a soil isolate to the onion model [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…From our results, we can speculate that the ability of B. cenocepacia to survive and replicate in various growth niches is due to the high genomic plasticity of this bacterial species and to the expression of host specific virulence factors. When properly regulated, this may help it to compete for survival in different settings [ 41 ]. As also suggested by Koskiniemi and colleagues [ 48 ], following growth inside a host selection can benefit bacterial mutants through an altered expression of virulence genes that better suit the environment of the host.…”

Section: Discussionmentioning

confidence: 99%

Environmental Burkholderia cenocepacia Strain Enhances Fitness by Serial Passages during Long-Term Chronic Airways Infection in Mice

Bragonzi

Paroni

Pirone

et al. 2017

IJMS

View full text Add to dashboard Cite

Burkholderia cenocepacia is an important opportunistic pathogen in cystic fibrosis (CF) patients, and has also been isolated from natural environments. In previous work, we explored the virulence and pathogenic potential of environmental B. cenocepacia strains and demonstrated that they do not differ from clinical strains in some pathogenic traits. Here, we investigated the ability of the environmental B. cenocepacia Mex1 strain, isolated from the maize rhizosphere, to persist and increase its virulence after serial passages in a mouse model of chronic infection. B. cenocepacia Mex1 strain, belonging to the recA lineage IIIA, was embedded in agar beads and challenged into the lung of C57Bl/6 mice. The mice were sacrificed after 28 days from infection and their lungs were tested for bacterial loads. Agar beads containing the pool of B. cenocepacia colonies from the four sequential passages were used to infect the mice. The environmental B. cenocepacia strain showed a low incidence of chronic infection after the first passage; after the second, third and fourth passages in mice, its ability to establish chronic infection increased significantly and progressively up to 100%. Colonial morphology analysis and genetic profiling of the Mex1-derived clones recovered after the fourth passage from infected mice revealed that they were indistinguishable from the challenged strain both at phenotypic and genetic level. By testing the virulence of single clones in the Galleria mellonella infection model, we found that two Mex1-derived clones significantly increased their pathogenicity compared to the parental Mex1 strain and behaved similarly to the clinical and epidemic B. cenocepacia LMG16656T. Our findings suggest that serial passages of the environmental B. cenocepacia Mex1 strain in mice resulted in an increased ability to determine chronic lung infection and the appearance of clonal variants with increased virulence in non-vertebrate hosts.

show abstract

Burkholderia cepacia Complex Regulation of Virulence Gene Expression: A Review

Cited by 38 publications

References 103 publications

Cervicovaginal microbiota composition correlates with the acquisition of high‐risk human papillomavirus types

Cervicovaginal microbiota composition correlates with the acquisition of high‐risk human papillomavirus types

Epidemiological investigation and successful management of a Burkholderia cepacia outbreak in a neurotrauma intensive care unit

Environmental Burkholderia cenocepacia Strain Enhances Fitness by Serial Passages during Long-Term Chronic Airways Infection in Mice

Contact Info

Product

Resources

About