Burkholderia pseudomallei and melioidosis

Meumann, Ella M.; Limmathurotsakul, Direk; Dunachie, Susanna J.; Wiersinga, Willem J.; Currie, Bart J.

doi:10.1038/s41579-023-00972-5

Cited by 48 publications

(47 citation statements)

References 194 publications

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“…It is well established that an early diagnosis is essential for the successful management of melioidosis by triggering timely treatment, which in turn reduces the mortality risk [10].…”

Section: Discussionmentioning

confidence: 99%

“…Currently, melioidosis is diagnosed by a culture technique, which could be performed either by culturing the specimens (e.g., blood, urine, pus) directly on selective media such as Ashdown's agar or by growing bacteria using a hemoculture bottle followed by identification with selective media or other techniques [10,35,36]. Thus, we first tested the selected LFIAs with a normal serum.…”

Section: Discussionmentioning

confidence: 99%

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Development of an Antigen Capture Lateral Flow Immunoassay for the Detection of Burkholderia pseudomallei

Nualnoi,

Wongwitwichot,

Kaewmanee

et al. 2024

Diagnostics

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Early diagnosis is essential for the successful management of Burkholderia pseudomallei infection, but it cannot be achieved by the current gold standard culture technique. Therefore, this study aimed to develop a lateral flow immunoassay (LFIA) targeting B. pseudomallei capsular polysaccharide. The development was performed by varying nitrocellulose membrane reaction pads and chase buffers. The prototype LFIA is composed of Unisart CN95 and chase buffer containing tris-base, casein, and Surfactant 10G. The assay showed no cross-reactivity with E. coli, S. aureus, P. aeruginosa, and P. acne. The limit of detections (LODs) of the prototype LFIA was 107 and 106 CFU/mL B. pseudomallei in hemoculture medium and artificial urine, respectively. These LODs suggest that this prototype can detect melioidosis from positive hemoculture bottles but not straight from urine. Additionally, these LODs are still inferior compared to Active Melioidosis Detect (AMDTM). Overall, this prototype holds the potential to be used clinically with hemoculture bottles. However, further improvements should be considered, especially for use with urine samples.

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“…It is well established that an early diagnosis is essential for the successful management of melioidosis by triggering timely treatment, which in turn reduces the mortality risk [10].…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development of an Antigen Capture Lateral Flow Immunoassay for the Detection of Burkholderia pseudomallei

Nualnoi,

Wongwitwichot,

Kaewmanee

et al. 2024

Diagnostics

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“…pseudomallei. This telluric bacterium can affect both humans and animals through inhalation, ingestion, or skin contact [1]. It is listed as a Class 1 pathogen by the U.S.…”

Section: Introductionmentioning

confidence: 99%

“…pseudomallei . This telluric bacterium can affect both humans and animals through inhalation, ingestion, or skin contact [1]. It is listed as a Class 1 pathogen by the U.S. Centers for Disease Control and Prevention (CDC) and as a selected agent under the Microorganisms and Toxins (MOT) regulations by the French National Agency for the Safety of Medicines and Health Products (ANSM).…”

Section: Introductionmentioning

confidence: 99%

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Serological screening in animals combined with environmental surveys provides definite proof of the local establishment ofBurkholderia pseudomalleiin Guadeloupe

Gasqué,

Guernier-Cambert,

Manuel

et al. 2024

Preprint

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Background: Melioidosis is an emerging infectious disease caused by the soil-dwelling bacterium Burkholderia pseudomallei that affects both humans and animals. It is endemic in South and Southeast Asia, and northern Australia, causing an estimated 165,000 human cases annually worldwide. Human cases have been reported in the French West Indies (Martinique and Guadeloupe) since the 1990s. Conversely, no human cases have been reported in French Guiana, a French territory in South America. Our study aimed to investigate whether B. pseudomallei is locally established in Guadeloupe and French Guiana. We assessed animal exposure by serology and examined the presence of B. pseudomallei in the environment of seropositive animals. Methodology/Principal findings: Blood samples were collected from domestic animals in two goat farms in Les Saintes, Guadeloupe (n=31), and in 56 farms in French Guiana (n=670) and tested by ELISA. Serological follow up was performed on selected farms. Soil, water and goat rectal swabs were collected and analysed by culture and PCR. In French Guiana, the highest prevalence rates were observed in equids (24%) and cattle (16%), while in Les Saintes, a prevalence of 39% was observed in goats. The longitudinal study in Les Saintes revealed consistent high seropositivity in goats. A B. pseudomallei strain was isolated from the soil from one of the farms and detected in goat rectal swabs from the other farm. Conclusions/Significance: Our environmental investigation prompted by the serologic data confirms the presence of B. pseudomallei in Les Saintes, consistent with documented human cases of melioidosis on this island. In French Guiana, our serologic results call for environmental surveys and a re-evaluation of human infections with melioidosis-like symptoms. The approach developed in this study may help to identify high-risk areas that warrant further investigation.

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Threat of Human Pathogens in Farmlands: A One Health Perspective

Jiang,

Yang,

Pommier

et al. 2024

J of Sust Agri & Env

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A key aspect of “One Health” is to recognize that environmental health and human health are inseparable. In this interconnected network, farmland plays a crucial role, as it serves as a link connecting various ecosystems. However, the threat of human pathogens in farmlands is often overlooked despite growing evidence highlighting its importance as a pressing public health concern via direct and indirect pathways. To address this issue, we advocate the adoption of a “One Health” approach, which relies on strengthening the development of detection tools and emphasizing the importance of sustainable agricultural practices aimed at enhancing soil biodiversity. By doing so, we can effectively disrupt the transmission source and mitigate the risks posed by human pathogens in agricultural ecosystems, ultimately safeguarding both human health and agricultural sustainability.

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Burkholderia pseudomallei and melioidosis

Cited by 48 publications

References 194 publications

Development of an Antigen Capture Lateral Flow Immunoassay for the Detection of Burkholderia pseudomallei

Development of an Antigen Capture Lateral Flow Immunoassay for the Detection of Burkholderia pseudomallei

Serological screening in animals combined with environmental surveys provides definite proof of the local establishment ofBurkholderia pseudomalleiin Guadeloupe

Threat of Human Pathogens in Farmlands: A One Health Perspective

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