Burkholderia pseudomallei Detection among Hospitalized Patients, Sarawak

Choi, Jessica; Hii, King Ching; Bailey, Emily S.; Chuang, Jia Yun; Tang, Wei; Wong, Edmund Kwang Yuen; Ti, Tiana; Pau, Kat Siong; Berita, Antoinette; Saihidi, Izreena; Ting, Jakie; Chua, Tiing-Tiing; Toh, Teck-Hock; AuCoin, David P.; DeShazer, David; Gray, Gregory C.

doi:10.4269/ajtmh.19-0625

Cited by 8 publications

(3 citation statements)

References 22 publications

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“…Recent studies reported that several nosocomial infections caused by BCC in countries like Canada, Australia, Thailand, Switzerland, and Saudi Arabia with a few cases leading to the death of the patients 4–7 . Previous reports have substantiated that in the USA and Canada, BCC infections among CF patients were prevalent with an average of 67%, and similar cases have been reported in New Zealand, Australia, and several European countries 3,8 .…”

Section: Introductionmentioning

confidence: 78%

Computational design of prospective molecular targets for Burkholderia cepacia complex by molecular docking and dynamic simulation studies

Skariyachan¹,

Praveen

Uttarkar³

et al. 2023

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The study aimed to screen prospective molecular targets of BCC and potential natural lead candidates as effective binders by computational modeling, molecular docking, and dynamic (MD) simulation studies. Based on the virulent functions, tRNA 5‐methylaminomethyl‐2‐thiouridine biosynthesis bifunctional protein (mnmC) and pyrimidine/purine nucleoside phosphorylase (ppnP) were selected as the prospective molecular targets. In the absence of experimental data, the three‐dimensional (3D) structures of these targets were computationally predicted. After a thorough literature survey and database search, the drug‐likeness, and pharmacokinetic properties of 70 natural molecules were computationally predicted and the effectual binding of the best lead molecules against both the targets was predicted by molecular docking. The stabilities of the best‐docked complexes were validated by MD simulation and the binding energy calculations were carried out by MM‐GBSA approaches. The present study revealed that the hypothetical models of mnmC and ppnP showed stereochemical accuracy. The study also showed that among 70 natural compounds subjected to computational screening, Honokiol (3′,5‐Di(prop‐2‐en‐1‐yl) [1,1′‐biphenyl]‐2,4′‐diol) present in Magnolia showed ideal drug‐likeness, pharmacokinetic features and showed effectual binding with mnmC and ppnP (binding energies −7.3 kcal/mol and −6.6 kcal/mol, respectively). The MD simulation and GBSA calculation studies showed that the ligand‐protein complexes stabilized throughout tMD simulation. The present study suggests that Honokiol can be used as a potential lead molecule against mnmC and ppnP targets of BCC and this study provides insight into further experimental validation for alternative lead development against drug resistant BCC.

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Section: Introductionmentioning

confidence: 78%

Computational design of prospective molecular targets for Burkholderia cepacia complex by molecular docking and dynamic simulation studies

Skariyachan¹,

Praveen

Uttarkar³

et al. 2023

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“…Therefore, identifying B. pseudomallei and other closely related Burkholderiaceae isolates is a considerable challenge for clinical microbiology laboratories ( Thibault et al., 2004 ). The culture-based method is the gold standard for identifying B. pseudomallei , but it usually takes 3-5 days; therefore, it is easy to miss the optimal time for treatment ( Lee et al., 2005 ; Choi et al., 2020 ). The ELISA method can be used to detect the specific antibodies to B. pseudomallei ( Kohler et al., 2016 ; Suttisunhakul et al., 2016 ; Yatsomboon et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Preliminary Evaluation of Rapid Visual Identification of Burkholderia pseudomallei Using a Newly Developed Lateral Flow Strip-Based Recombinase Polymerase Amplification (LF-RPA) System

Shang

et al. 2022

Front. Cell. Infect. Microbiol.

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Burkholderia pseudomallei is an important infectious disease pathogen that can cause melioidosis. Melioidosis is mainly prevalent in Thailand, northern Australia and southern China and has become a global public health problem. Early identification of B. pseudomallei is of great significance for the diagnosis and prognosis of melioidosis. In this study, a simple and visual device combined with lateral flow strip-based recombinase polymerase amplification (LF-RPA) was developed, and the utility of the LF-RPA assay for identifying B. pseudomallei was evaluated. In order to screen out the optimal primer probe, a total of 16 pairs of specific primers targeting the orf2 gene of B. pseudomallei type III secretion system (T3SS) cluster genes were designed for screening, and F1/R3 was selected as an optimal set of primers for the identification of B. pseudomallei, and parameters for LF-RPA were optimized. The LF-RPA can be amplified at 30-45°C and complete the entire reaction in 5-30 min. This reaction does not cross-amplify the DNA of other non-B. pseudomallei species. The limit of detection (LOD) of this assay for B. pseudomallei genomic DNA was as low as 30 femtograms (fg), which was comparable to the results of real-time PCR. Moreover, 21 clinical B. pseudomallei isolates identified by 16S rRNA gene sequencing were retrospectively confirmed by the newly developed LF-RPA system. Our results showed that the newly developed LF-RPA system has a simple and short time of operation and has good application prospect in the identification of B. pseudomallei.

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“…Bacterial culture, although specific, may lack sensitivity due to both inadequate sampling and low bacterial loads in clinical samples (Hoffmaster et al, 2015). Other methods of laboratory diagnosis, including direct real-time polymerase chain reaction assays and rapid antigen detection, have not been shown to be more sensitive than bacterial culture (Choi et al, 2020;Currie, 2015). Serological diagnosis, meanwhile, is complicated by the high background sero-positivity among individuals living in endemic areas (Norazah et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Detection of spleen abscesses facilitates diagnosis of melioidosis in Malaysian children

Mohan

Manan

Tan

et al. 2020

International Journal of Infectious Diseases

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Melioidosis is associated with extremely high case fatality ratios. The aim of this study was to determine whether detection of abdominal visceral abscesses can facilitate diagnosis of melioidosis in children. Methods: We conducted a retrospective analysis of all children who had liver and/or spleen abscesses on abdominal ultrasonography admitted to Bintulu Hospital in Sarawak, Malaysia, from January 2014 until December 2018. Results: Fifty-three children had liver and/or spleen abscesses. Spleen abscesses were present in 48 (91%) cases; liver abscesses in 15 (28%). Melioidosis was confirmed by culture in 9 (17%) children; small occult splenic abscesses were present in all cases. In 78% of these cases, the lesions were detected before any positive culture (or serology) results were available. Four (8%) children had bacteriologically-confirmed tuberculosis. Two (4%) had Staphylococcus aureus infection. Of the remaining 38 (72%) culture-negative cases, 36 (95%) had clinical and imaging characteristics similar to that of children with culture-confirmed melioidosis and improved with empirical melioidosis antibiotic therapy. Conclusions: A large number of children in Bintulu Hospital in Sarawak, Malaysia, were found to have spleen abscesses. Melioidosis was the most common etiology identified in these children. Abdominal ultrasonography is extremely useful in facilitating the diagnosis of pediatric melioidosis.

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Burkholderia pseudomallei Detection among Hospitalized Patients, Sarawak

Cited by 8 publications

References 22 publications

Computational design of prospective molecular targets for Burkholderia cepacia complex by molecular docking and dynamic simulation studies

Computational design of prospective molecular targets for Burkholderia cepacia complex by molecular docking and dynamic simulation studies

Preliminary Evaluation of Rapid Visual Identification of Burkholderia pseudomallei Using a Newly Developed Lateral Flow Strip-Based Recombinase Polymerase Amplification (LF-RPA) System

Detection of spleen abscesses facilitates diagnosis of melioidosis in Malaysian children

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