Burkholderia silvatlantica sp. nov., a diazotrophic bacterium associated with sugar cane and maize

Perin, Liamara; Martínez‐Aguilar, Lourdes; Paredes-Valdez, Guadalupe; Baldani, José Ivo; Santos, Paulina Estrada‐de los; Reis, Verônica Massena; Caballero‐Mellado, Jesús

doi:10.1099/ijs.0.64362-0

Cited by 129 publications

(107 citation statements)

References 30 publications

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“…The type strain, FeGl01 T (=LMG 23612 T =CECT 7171 T =DSM 18251 T ), was isolated from ore material from the Jangada mine, Minas Gerais State, Brazil. (Reis et al, 2004); 5, B. silvatlantica (Perin et al, 2006); 6, B. mimosarum (Chen et al, 2006). +, Good growth; ±, poor growth; 2, no growth; V, variable; ND, no data available.…”

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Burkholderia ferrariae sp. nov., isolated from an iron ore in Brazil

Valverde

Delvasto

Peix

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative, non-spore-forming bacterial strain with the ability to solubilize highly insoluble phosphatic minerals was isolated from a high-phosphorous iron ore from Minas Gerais State, Brazil. This strain, designated FeGl01T, was subjected to a polyphasic taxonomic investigation. Comparative 16S rRNA gene sequence analysis indicated that it formed a distinct phylogenetic lineage within the genus Burkholderia together with several other species of the genus, e.g. Burkholderia sacchari, Burkholderia tropica and Burkholderia unamae. Partial nucleotide sequencing and analysis of the recA gene roughly corroborated the phylogenetic position of strain FeGl01T within the genus Burkholderia. The chemotaxonomic properties of strain FeGl01T, such as ubiquinone Q-8 as the predominant quinone system and C16 : 0, C17 : 0 cyclo, C18 : 1 ω7c and C19 : 0 ω8c cyclo as the major fatty acids, were also consistent with its classification within the genus Burkholderia. DNA–DNA hybridization experiments between strain FeGl01T and the type strains of B. unamae, B. sacchari and B. tropica yielded reassociation values of 40 % or lower, which, together with qualitative and quantitative differences in fatty acid composition and with differences in several phenotypic traits, support the separation of the new isolate from the phylogenetically most closely related species. Therefore, it is suggested that strain FeGl01T represents a novel species of the genus Burkholderia, for which the name Burkholderia ferrariae sp. nov. is proposed. The type strain is FeGl01T (=LMG 23612T=CECT 7171T=DSM 18251T).

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“…+, Good growth; ±, poor growth; 2, no growth; V, variable; ND, no data available. silvatlantica (Perin et al, 2006).…”

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confidence: 99%

Burkholderia ferrariae sp. nov., isolated from an iron ore in Brazil

Valverde

Delvasto

Peix

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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“…Representatives of G. diazotrophicus species were isolated from all sugarcane varieties while B. tropica was only obtained from RB72454 and RB867515. Many diazotrophic Burkholderia species were isolated and described from sugarcane samples grown in Brazil, B. tropica (Reis et al, 2004), B. unamae and B. silvatlantica (Perin et al, 2006).…”

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Diazotrophic bacteria associated to sugarcane varieties cropped at Northeast Region of Brazil

Antonio

Rouws

Teixeira

et al. 2016

Agraria

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The objective of this work was to study the diversity of diazotrophic bacteria associated to 10 varieties of sugarcane cultivated in two states of the Northeast of Brazil and to identify the isolates by morphological and molecular methods. Diversity was studied by the application of traditional growth-depending microbiological methods: Gram-staining, comparison of colony morphology and growth on several culture media. Phylogenetic taxonomy was performed using 16S rRNA gene sequencing data. Almost all isolates obtained in semi-solid nitrogen free media were classified as Gram-negative and seven colony morphological groups were distinguished after growth on Potato medium. Among the 10 varieties evaluated, RB72454 resulted in higher number of isolates and diversity. The great majority of isolates from most of the genotypes tested showed similar characteristic to the genus Gluconacetobacter based on its peculiar colony morphology and growth characteristics in semi-solid media. Comparison of the 16S rRNA sequence of each isolate to the Genbank showed that they are closely related to Gluconacetobacter, Burkholderia, Kosakonia, Klebsiella and Stenotrophomonas.Key words: biological nitrogen fixation, ecology of diazotrophic bacteria, sugarcane Bactérias diazotróficas associadas a variedades de cana-de-açúcar cultivadas na região Nordeste do Brasil RESUMOO objetivo deste trabalho foi estudar a diversidade de bactérias diazotróficas associadas a 10 variedades de cana cultivadas em dois estados do Nordeste do Brasil e identificar os isolados por métodos morfológicos e moleculares. A diversidade foi estudada através da aplicação de métodos microbiológicos tradicionais dependentes de crescimento: coloração de Gram, comparação da morfologia de colónias e crescimento em vários meios de cultura. Taxonomia filogenética foi realizada utilizando dados de sequenciamento de gene 16S rRNA. Quase todos os isolados obtidos em meios semissólidos livres de nitrogênio foram classificados como Gram-negativos e sete grupos baseados na morfologia de colônias foram distinguidos após crescimento em meio batata. Entre as 10 variedades avaliadas, RB72454 resultou em maior diversidade e número de isolados. A grande maioria dos isolados dos genótipos testados apresentaram característica similar ao gênero Gluconacetobacter com base em seu peculiar crescimento em meios semissólidos e morfologia da colônia. A comparação da sequência do 16S rRNA de cada isolado com sequencias do GenBank mostrou que eles estão intimamente relacionados com Gluconacetobacter,

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“…It is a genus that has a considerable physiological versatility and occupies a great variety of ecological niches (Perin et al, 2006a). Perin et al (2006b) report that the genus Burkholderia includes more than 30 species, but not all of them are able to fix N 2 . However, in the present study, all the lineages from this genus are N 2 fixing and most of them solubilize inorganic phosphate (Table 4).…”

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confidence: 99%

Bacteria associated with sugarcane in Northeastern Brazil

Silva¹,

Freire²,

Kuklinsky-Sobral³

et al. 2016

Afr. J. Microbiol. Res.

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Sugarcane has a high demand in nitrogen, increasing costs and causing damages to the environment. It is necessary to find alternatives to reduce nitrogen fertilizers use. Diazotrophic bacteria have capacity to promote growth in grass with potential to fix N 2 and solubilize inorganic phosphate. This study aimed to evaluate bacterial community associated with different sugarcane varieties in Northeastern Brazil, select bacteria with plant growth-promoting characteristics, and identify endophytic and epiphytic bacterial lineages in sugarcane. Endophytic bacteria of leaves and roots and epiphytic bacteria of rhizoplane were isolated from three sugarcane commercial varieties and selected for their capacity to fix N 2 and solubilize inorganic phosphate. Bacterial strains from different morphological groups were isolated and a sample of 27 strains with potential for the simultaneous development of these characteristics were selected and identified. The bacterial community that interacted with sugarcane was more associated with rhizoplane and roots regions than with leaves, and had a high potential to fix N 2 and solubilize inorganic phosphate. Bacterial lineages were mainly from genera Pantoea sp. and Burkholderia sp., but there were also genera Enterobacter sp., Klebsiella sp. and Pseudomonas sp. and two lineages at the species level: Pantoea stewartii and Burkholderia cenocepacia.

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Burkholderia silvatlantica sp. nov., a diazotrophic bacterium associated with sugar cane and maize

Cited by 129 publications

References 30 publications

Burkholderia ferrariae sp. nov., isolated from an iron ore in Brazil

Burkholderia ferrariae sp. nov., isolated from an iron ore in Brazil

Diazotrophic bacteria associated to sugarcane varieties cropped at Northeast Region of Brazil

Bacteria associated with sugarcane in Northeastern Brazil

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