Burkholderia thailandensis as a microbial cell factory for the bioconversion of used cooking oil to polyhydroxyalkanoates and rhamnolipids

Kourmentza, Constantina; Costa, Joana; Azevedo, Zélia; Servin, C.; Grandfils, Christian; Freitas, Víctor de; Reis, Maria A.M.

doi:10.1016/j.biortech.2017.09.138

Cited by 128 publications

(52 citation statements)

References 45 publications

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“…We identified the phaC gene as responsible for the PHA production in B. thailandensis . This is consistent with the PHA composition described in a recent study, where scl-PHA, also named polyhydoxybutyrate (PHB), were characterized (Kourmentza, Costa et al 2018).…”

Section: Optical Density At 600 Nm Is Not Representative For Growth Msupporting

confidence: 91%

“…PHA production in B. sacchari and B. cepacia were recently reported, with PHB contents around 88% and 74%, respectively, in a medium containing detoxified wood hydrolyzates (Kucera, Benesova et al 2017). Furthermore, Kourmentza et al (2018) reported that PHB content represents 60% of the dry cell mass when B. thailandensis was grown on used cooking oil (Kourmentza, Costa et al 2018). All together, these observations suggest that studies using optical density measurements for growth assessment might be inconsistent for Burkholderia species.…”

Section: Pha Production Is Responsible For the Od Difference Between mentioning

confidence: 99%

“…The soil saprophyte Burkholderia thailandensis was recently identified as a producer of polyhydroxyalkanoates (PHAs) (Funston, Tsaousi et al 2017) and then studied for the co-production of PHAs and rhamnolipid in used cook oil (Kourmentza, Costa et al 2018). PHAs are polymers of hydroxylated fatty acids that are synthesized by different prokaryotic microorganisms as intracellular carbon storage material (Anderson and Dawes 1990).…”

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confidence: 99%

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Polyhydroxyalkanoate granule accumulation makes optical density measurement an unreliable method for bacterial growth assessment inBurkholderia thailandensis

Martinez

Déziel

2019

Preprint

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Optical density (OD) measurement is the standard method used in microbiology for estimating bacterial concentrations in cultures. However, most studies do not compare these measurements with viable cell counts and assume that they reflect the real cell concentration. Burkholderia thailandensis was recently identified as a polyhydroxyalkanoate (PHA) producer. PHA biosynthesis seems to be coded by an ortholog of the Cupriavidus necator phaC gene. When growing cultures of wildtype strain E264 and an isogenic phaC- mutant, we noted a difference in their OD600 values, although viable cell counts indicated similar growth. Investigating the cellular morphologies of both strains, we found that under our conditions the wildtype strain was full of PHA granules, deforming the cells, while the mutant contained no granules. These factors apparently affected the light scattering, making the OD600 values no longer representative of cell density. We show a direct correlation between OD600 values and the accumulation of PHA. We conclude that OD measurement is unreliable for growth evaluation of B. thailandensis because of PHA production. This study also suggests that B. thailandensis could represent an excellent candidate for PHA bioproduction. Correlation between OD measurements and viable cell counts should be verified on any study realized in B. thailandensis.

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Section: Optical Density At 600 Nm Is Not Representative For Growth Msupporting

confidence: 91%

Section: Pha Production Is Responsible For the Od Difference Between mentioning

confidence: 99%

mentioning

confidence: 99%

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Polyhydroxyalkanoate granule accumulation makes optical density measurement an unreliable method for bacterial growth assessment inBurkholderia thailandensis

Martinez

Déziel

2019

Preprint

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“…Kourmentza et al, on the other hand, have reported co-production of P(3HB) polymers along with rhamnolipids when Burkholderia thailandensis was employed for the bioconversion of used cooking oil. It was reported that up to 60% of the cell biomass consisted of P(3HB) while 2.2 g/L of rhamnolipids were produced simultaneously (Kourmentza et al, 2018).…”

Section: Biosynthesis Of Phas Using Waste Plant Oilsmentioning

confidence: 99%

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

Surendran

Lakshmanan

Chee

et al. 2020

Front. Bioeng. Biotechnol.

121

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Polyhydroxyalkanoates (PHAs) are a potential replacement for some petrochemical-based plastics. PHAs are polyesters synthesized and stored by various bacteria and archaea in their cytoplasm as water-insoluble inclusions. PHAs are usually produced when the microbes are cultured with nutrient-limiting concentrations of nitrogen, phosphorus, sulfur, or oxygen and excess carbon sources. Such fermentation conditions have been optimized by industry to reduce the cost of PHAs produced commercially. Industrially, these biodegradable polyesters are derived from microbial fermentation processes utilizing various carbon sources. One of the major constraints in scaling-up PHA production is the cost of the carbon source metabolized by the microorganisms. Hence, cheap and renewable carbon substrates are currently being investigated around the globe. Plant and animal oils have been demonstrated to be excellent carbon sources for high yield production of PHAs. Waste streams from oil mills or the used oils, which are even cheaper, are also used. This approach not only reduces the production cost for PHAs, but also makes a significant contribution toward the reduction of environmental pollution caused by the used oil. Advancements in the genetic and metabolic engineering of bacterial strains have enabled a more efficient utilization of various carbon sources, in achieving high PHA yields with specified monomer compositions. This review discusses recent developments in the biosynthesis and classification of various forms of PHAs produced using crude and waste oils from the oil palm and fish industries. The biodegradability of the PHAs produced from these oils will also be discussed.

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“…The average molecular weight, of the PHA polymers, by weight (M w ) and by number (M n ), as well as the polydispersity index (PDI = M w /M n ) were determined by size exclusion chromatography (SEC), and Differential Scanning Calorimetry (DSC) and thermogravimetric analyses were performed as previously described (Kourmentza et al, 2018).…”

Section: Pha Molecular Mass Distribution and Thermal Propertiesmentioning

confidence: 99%

Occurrence of non-toxic bioemulsifiers during polyhydroxyalkanoate production by Pseudomonas strains valorizing crude glycerol by-product

Kourmentza

Araújo

Sevrin

et al. 2019

Bioresource Technology

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Burkholderia thailandensis as a microbial cell factory for the bioconversion of used cooking oil to polyhydroxyalkanoates and rhamnolipids

Cited by 128 publications

References 45 publications

Polyhydroxyalkanoate granule accumulation makes optical density measurement an unreliable method for bacterial growth assessment inBurkholderia thailandensis

Polyhydroxyalkanoate granule accumulation makes optical density measurement an unreliable method for bacterial growth assessment inBurkholderia thailandensis

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

Occurrence of non-toxic bioemulsifiers during polyhydroxyalkanoate production by Pseudomonas strains valorizing crude glycerol by-product

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