Burn Healing with Binahong (Anredera cordifolia (Tenore) Steenis) Leaves Extract as A Topical and Systemic Treatments

Abstract

Abstract-Burn is still an important problem in health facility worldwide. Burns management from ancients ago until now is quite varied either giving topical therapy alone or combination with systemic drug along with various clinical considerations. Research on the healing of burns with phytoextract has been done in the last 20 years. Binahong leaves or Anredera Cordifolia (Tenore) Steenis is one of the most studied phytoextract for wounds, but lack on burns research. Previous studies in this leaf ethanol extract showed that 40% concentration is the most effective on wound healing. Describes the effect of binahong 40% leaves ethanolic extract in the formation of reepithelialization, neo-vascularization, fibroblasts, and the density of collagen as part of healing process on post second degree burns of the skin as topical and systemic/oral treatments. Experimental study on 25 Sprague dawleys that divided into 5 groups. Group I was treated with ointment of binahong 40% leaves ethanolic extract, group II was treated with oral preparation of binahong leaves ethanolic extract 100mg/kg body weight, group III was treated with combination of binahong 40% leaves ethanolic extract both topical and oral preparations, group IV was treated with a silver sulfadiazine ointment (positive control), and group V was treated with ointment base (negative control). Treatment was started after second-degree burns created on each rat for 6 days. Burns site excised after six days of treatments, prepared for paraffin embedded and HE staining to examine tissue reepitelialization, neo-vascularisations, fibroblast count, collagen formation, and inflammatory cell formation using light microscope. There was no significant difference in the process of reepithelialization, neovascularization, the amount of fibroblasts, among five groups (p> 0.05). While the lowest inflammatory cells and the density of collagen were best seen in the 40% extract ointment group with p = 0, 0001 and p = 0.037 (p<0.05). The process of burns healing with topical and oral binahong extracts did not show any significant difference, but topical administration was able to suppress the number of inflammatory cells and increase collagen formation more effective than sistemic/oral treatments alone.

Senyawa Fitokimia dan Aktivitas Farmakologi Family Basellaceae sebagai Obat Luka : A Narrative Review

“…Steroid adalah senyawa organik yang larut dalam lemak, memiliki empat cincin yang dalam orientasi perhydrocyclopentano [α] fenantrena. Triterpenoid dan steroid pada family Basellaceae memiliki kemampuan untuk mempromosikan fibroblast yang akan mensintesis kolagen dan akan mendukung struktur daerah yang mengalami proses penyembuhan luka (Balafif et al, 2013;Borah dan Banik, 2020;Hanafiah et al, 2019;Hapsari et al, 2017;Reyes et al, 2017).…”

“…Senyawa ini juga menghambat 5-lipoksigenase, sehingga menghambat pembentukan leukotriene dari asam arakidonat. Reaksi inflamasi akan memendek, pembentukan kolagen meningkat, dan penyembuhan luka dapat dipercepat (Feng et al, 2020;Hapsari et al, 2017). Asam oleanolat juga dapat meningkatkan aktivitas cicatrizant pada proses penyembuhan luka.…”