Burst pressure of phaseguide structures of different heights in all-polymer microfluidic channels

Garbarino, Francesca; Kistrup, Kasper; Rizzi, Giovanni; Hansen, Mikkel Fougt

doi:10.1088/1361-6439/aa97b7

Cited by 8 publications

(5 citation statements)

References 13 publications

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“…In our previous study, 31 we demonstrated sequential sample dispensing into five microchambers (volume = 3 µL) by controlling the burst pressures in three types of phaseguide ridge structures (hereinafter referred to as lateral-type phaseguides 33,34 ) with different inclined angles against the flow direction, as presented in Fig. S1.…”

Section: Vertical-type Phaseguide Structure As a Passive Valvementioning

confidence: 99%

A method of sequential liquid dispensing for the multiplexed genetic diagnosis of viral infections in a microfluidic device

et al. 2021

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Section: Vertical-type Phaseguide Structure As a Passive Valvementioning

confidence: 99%

A method of sequential liquid dispensing for the multiplexed genetic diagnosis of viral infections in a microfluidic device

et al. 2021

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“…The tumor section was surrounded by two microfluidic channels (1 mm width × 800 μm height) for the delivery of oxygen, nutrients, and therapeutic drugs and/or immune cells (Figure D,G). Cancer cells were loaded through cell injection ports to create 2-D and 3-D tumor sections by a liquid-pinning process, where a drop of cells with a desired number (in medium, for 2-D) or density (in ECM gel before curing) was injected and held underneath the central pillar by the surface tension at the liquid–air interface − (Figure E,F). The device geometry and liquid pinning created a micropattern of cancer cell layer/bulk with the desired shape, area, and/or volume as a tumor model.…”

Section: Resultsmentioning

confidence: 99%

“…Liquid pinning − was chosen to create a cancer cell monolayer or 3-D tumor section in our tumor model, for its ease of operation without requiring additional structural components (such as pillars) that may interfere with oxygen diffusion. It utilized surface tension at the liquid–substrate–air interface to hold the liquid within the small crevices of the device (i.e., the diffusion gap in the current design) (Figure D).…”

Section: Resultsmentioning

confidence: 99%

Recapitulating Tumor Hypoxia in a Cleanroom-Free, Liquid-Pinning-Based Microfluidic Tumor Model

Begum

Liu

et al. 2022

ACS Biomater. Sci. Eng.

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In tumors, the metabolic demand of cancer cells often outpaces oxygen supply, resulting in a gradient of tumor hypoxia accompanied with heterogeneous resistance to cancer therapeutics. Models recapitulating tumor hypoxia are therefore essential for developing more effective cancer therapeutics. Existing in vitro models often fail to capture the spatial heterogeneity of tumor hypoxia or involve high-cost, complex fabrication/handling techniques. Here, we designed a highly tunable microfluidic device that induces hypoxia through natural cell metabolism and oxygen diffusion barriers. We adopted a cleanroom-free, micromillingreplica-molding strategy and a microfluidic liquid-pinning approach to streamline the fabrication and tumor model establishment. We also implemented a thin-film oxygen diffusion barrier design, which was optimized through COMSOL simulation, to support both two-dimensional (2-D) and three-dimensional (3-D) hypoxic models. We demonstrated that liquid-pinning enables an easy, injection-based micropatterning of cancer cells of a wide range of parameters, showing the high tunability of our design. Human breast cancer and prostate cancer cells were seeded and stained after 24 h of 2-D and 3-D culture to validate the natural induction of hypoxia. We further demonstrated the feasibility of the parallel microfluidic channel design to evaluate dual therapeutic conditions in the same device. Overall, our new microfluidic tumor model serves as a user-friendly, cost-effective, and highly scalable platform that provides spatiotemporal analysis of the hypoxic tumor microenvironments suitable for high-content biological studies and therapeutic discoveries.

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“…1d, where the NDG configuration is represented. CBVs are strictions within microfluidic channels providing a pinning interface for a forward proceeding fluid [19][20][21] . Classically, CBVs were designed to stop an advancing front from going further on, left or right 21,22 .…”

Section: A Device For Mechanical Compression Of Bi-layered 3d Microti...mentioning

confidence: 99%

Modelling Osteoarthritis pathogenesis through Mechanical Loading in an Osteochondral Unit-on-Chip

Mainardi,

Börsch,

Occhetta

et al. 2023

Preprint

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A cure for osteoarthritis (OA), the most prevalent musculoskeletal disease, remains an unmet need. Investigating the molecular and cellular processes leading to OA is challenged by the absence of human models that capture the complex interplay among different tissues in the joint under pathophysiological mechanical loading.In this study, we have engineered an OsteoChondral Unit (OCU)-on-chip system where composite hyaline cartilage - mineralized osseous microtissue analogues are exposed to controlled, tissue-specific compression regimes akin to those of the OCUin vivo. Through single-cell transcriptomic analysis, we demonstrate the critical relevance of the mineralized layer in inducing chondrocyte subpopulations implicated in the progression of OA.Upon exposure to hyperphysiological loading, the OCU-on-chip captures early phenotypic traits of OA pathogenesis, comprising alterations of subchondral mineral content and acquisition of previously described OA genetic signatures.This system enabled to identify novel upstream drivers of OA metabolic changes, including mechanically induced ribosomal alterations, as well as associated molecular targets towards the development of disease-modifying OA therapies.

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Burst pressure of phaseguide structures of different heights in all-polymer microfluidic channels

Cited by 8 publications

References 13 publications

A method of sequential liquid dispensing for the multiplexed genetic diagnosis of viral infections in a microfluidic device

A method of sequential liquid dispensing for the multiplexed genetic diagnosis of viral infections in a microfluidic device

Recapitulating Tumor Hypoxia in a Cleanroom-Free, Liquid-Pinning-Based Microfluidic Tumor Model

Modelling Osteoarthritis pathogenesis through Mechanical Loading in an Osteochondral Unit-on-Chip

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