Bursting and spreading of liposomes on the surface of a static mercury drop electrode

Hellberg, Dirk; Scholz, Fritz; Schauer, Frieder; Weitschies, Werner

doi:10.1016/s1388-2481(02)00279-5

Cited by 105 publications

(78 citation statements)

References 14 publications

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“…The works builds on the pioneering work of Scholz and co-workers who investigated the non-Faradaic adhesion of undoped liposomes on a static mercury-drop electrode. [6] The Faradaic oxidation of ferrocene-doped oil droplets has also been reported. [4e] First, a voltammetric method was employed to determine the amount of free vitamin C and the total amount of vitamin C in a liposome suspension (see the Supporting Information).…”

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confidence: 99%

Investigation of Single‐Drug‐Encapsulating Liposomes using the Nano‐Impact Method

2014

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confidence: 99%

Investigation of Single‐Drug‐Encapsulating Liposomes using the Nano‐Impact Method

2014

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“…As has been reported previously [16,17,21], the adhesion, rupture and spreading of multilamellar and giant unilamellar vesicles (MLVs and GUVs, respectively) on mercury electrodes can be characterized with chronoamperometric measurements. As liposomes rupture and spread on mercury electrodes forming a supported lipid monolayer [18], the rupture of single liposomes produces adsorbed lecithin islands on the mercury surface.…”

Section: The Adhesion-spreading Of Liposomes On Mercury Electrodesmentioning

confidence: 80%

“…Therefore, the formation of the individual islands is coupled with the flow of capacitive currents, which are recorded as spikes in chronoamperometric measurements [16,17] as shown in Fig. 2a.…”

Section: The Adhesion-spreading Of Liposomes On Mercury Electrodesmentioning

confidence: 99%

“…In other words, a general consensus is still lacking. The situation is even more complex when referring to the adhesion, rupture and spreading of liposomes on hydrophobic substrates, where the formation of supported lipid bilayers [13][14][15], supported lipid monolayers [16][17][18], and immobilized liposome layers [10,19] has been reported. The level of hydrophobicity and smoothness of the surface being studied have been proposed as possible reasons behind these discrepancies [13].…”

Section: Introductionmentioning

confidence: 99%

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The theory of metal electronucleation applied to the study of fundamental properties of liposomes

Hernández

2012

J Solid State Electrochem

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“…Scholz was the first who studied the collisions of liposomes with a mercury electrode by recording current transients observed when liposomes contacted the electrode, burst and spread on its surface. 145 Heyrovsky in 2006, demonstrated detection of faradaic current transients during collisions of metal colloids particles for the first time. 146 The methodology was further developed by Bard 147 and Compton 148 using two different approaches.…”

Section: Collision-based Electrochemistrymentioning

confidence: 99%

Facilitating electron transfer in bioelectrocatalytic systems

Sekretaryova¹

2016

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During the course of the research underlying this thesis, Alina Sekretaryova was enrolled in Forum Scientium, a multidisciplinary doctoral programme at Linköping University, Sweden Alina Sekretaryova, 2016, unless otherwise noted. Cover design by Alina Sekretaryova Linköping studies in science and technology Dissertation No. 1738 Printed in Sweden by LiU-Tryck, Linköping, Sweden, 2016 iii ABSTRACT Bioelectrocatalytic systems are based on biological entities, such as enzymes, whole cells, parts of cells or tissues, which catalyse electrochemical processes that involve the interaction between chemical change and electrical energy. In all cases, enzymes, isolated or residing inside cells or part of cells, implement biocatalysis. Electron transfer phenomena, within the protein molecules and between biological redox systems and electronics, enable the development of various bioelectrocatalytic systems, which can be used both for fundamental investigations of enzymatic biological processes by electrochemical methods and for applied purposes, such as power generation, bioremediation, chemical synthesis and biosensing.Electrical communication between the biocatalysts redox centre and an electrode is essential for the functioning of the system. This can be established using two main mechanisms: indirect electron transfer and direct electron transfer. The efficiency of the electron transfer influences important parameters such as the turnover rate of the biocatalyst, the generated current density and partially the stability of the system. These in turn determine the response time, sensitivity, detection limit and operational stability of biosensors or the power densities and current output of biofuel cells, and hence they should be carefully considered when designing bioelectrocatalytic systems.This thesis focuses on approaches that facilitate electron transfer in bioelectrocatalytic systems based on mediated and direct electron transfer mechanisms. Both fundamental aspects of electron transfer in bioelectrocatalytic systems and applications of such systems for biosensing and power generation are considered. First, a new hydrophobic mediator for oxidases, unsubstituted phenothiazine, and its improved electron transfer properties in comparison with commonly used mediators are discussed. Application of the mediator in electrochemical biosensors is demonstrated by glucose, lactate and cholesterol sensing. Utilisation of mediated biocatalytic cholesterol oxidation as the anodic reaction for the construction of a biofuel cell, acting as a power supply and an analytical device at the same time, is investigated as a "self-powered" biosensor. In addition, the enhancement of mediated bioelectrocatalysis by the employment of microelectrodes as a transducer is examined. The effect of surface roughness on the current response of the microelectrodes under conditions of convergent diffusion is considered. The applicability of the laccase-based system for total phenol analysis of weakly supported water is demonstrated. Finally, a...

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Bursting and spreading of liposomes on the surface of a static mercury drop electrode

Cited by 105 publications

References 14 publications

Investigation of Single‐Drug‐Encapsulating Liposomes using the Nano‐Impact Method

Investigation of Single‐Drug‐Encapsulating Liposomes using the Nano‐Impact Method

The theory of metal electronucleation applied to the study of fundamental properties of liposomes

Facilitating electron transfer in bioelectrocatalytic systems

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