Buserelin Acetate Reduces Mortality and DNA Defragmentation of Bovine Sperm Cells Exposed to Oxidative Stress

Abstract

Authors' Contribution CZ and BW are the main contributors of this research. JR is the person in charge of this project. QS and MMAR revised the manuscript.The giant panda Ailuropoda melanoleuca is one of the most iconic mammals in the world. The species has experienced declines in its habitat and population due to human disturbance. To protect this species, we investigated the relationship between giant panda habitat use intensity and human disturbance density in the Daxiangling Mountains. The results indicated that, among multiple kinds of disturbances, roads affected the giant panda habitat use significantly. In addition, roads caused the giant panda habitat use intensity to decline sharply. The giant panda nearly stopped using the habitat when road density was more than 0.4 km/km 2 . Thus, road density should be considered in the protection program. Furthermore, in areas inhabited by giant pandas, we recommend to optimizing and enhancing increased regulations to minimize the expansion and impact of roads.

Evaluation of the effectiveness of the use of exosomes in the regulation of the mitochondrial membrane potential of frozen/thawed spermatozoa

“…To a second tube placed on ice, 50 μl of this mixture was measured and 100 ml of acidic detergent solution (0.08 M HCl, 0.15 M NaCl, 0.1% v/v Triton X-100, pH 1.2) was added and incubated for 30 seconds in the dark. Na 2 HPO 4 per ml of citrate buffer (0.037 M citric acid, 0.126 M, 1.1 mM disodium EDTA, 0.15 M NaCl, pH 6.0) was added to the prepared mixture and incubated on ice for another 3 minutes, followed by cytometric analysis [ 14 ].…”

“…Semen samples were centrifuged at 750 rpm for 10 min to remove the supernatants, obtained cell pellets were washed three times with PBS and then incubated with 0.2% Triton X-100 on ice for 20 min. Superoxide dismutase (SOD) and catalase (CAT) activities were determined by spectrophotometry, by commercially available enzyme activity detection kits (Jiancheng Institute of Bioengineering), and the malondialdehyde (MDA) by thiobaby Tauric acid (TBA) assay using a chemical reaction kit (Built Bioengineering Institute) following the manufacturer’s instructions, as previously described [ 14 ].…”

Fertility Rate and Assessment of the Cytoprotective Capacity of Various Types of Holothuroidea Extracts on Spermatozoa

“…During the process, oxidative and osmotic stress occurs, which results in the production of reactive oxygen species (ROS) [9,10]. Sperm subjected to processing and subsequent storage at low temperatures experience a number of damage, including the loss of the integrity of the cytoplasmic membrane, DNA and acrosome defragmentation [11], which results in a reduction in the viability and motility of sperm cells [11,12]. The reduced quality of semen affects the effectiveness of its use in assisted reproduction (alternative insemination).…”