Bushen Ningxin Decoction pharmacological serum promotes the proliferation and suppresses the apoptosis of murine osteoblasts through MAPK pathway

Bu-Shen-Ning-Xin decoction suppresses osteoclastogenesis <i>via </i>increasing dehydroepiandrosterone to prevent postmenopausal osteoporosis

“…In order to ascertain the effects of BSNXD on PMO, an animal model of ovariectomized (OVX) female mice was used to deplete ovarian hormones. The current authors previously found that pharmacological serum from OVX mice treated with BSNXD promoted the proliferation and inhibited the apoptosis of mouse osteoblasts (7). The aim of the present study was to investigate how BSNXD improved PMO in terms of osteoclasts in OVX mice.…”

“…BSNXD has a beneficial effect on bone metabolism (7), but the mechanisms of that effect are unclear. In order to ascertain the effects of BSNXD on PMO, an animal model of ovariectomized (OVX) female mice was used to deplete ovarian hormones.…”

Fuzheng Huayu recipe and vitamin E reverse renal interstitial fibrosis through counteracting TGF-β1-induced epithelial-to-mesenchymal transition

“…In this experiment, the sera were collected from rats which took FZHY and was used as medicine for incubating the cells in order to investigate the effects of FZHY on cultured cells. This is called serum pharmacological method, which was first introduced by Japanese for herbal medicine research in vitro (Iwama et al, 1987), and have been used widely and successively in recent years (Shen et al, 2007;Wang et al, 2009), with the advantages of mimicking real action of herbal medicine in vivo and of avoiding non-pharmacological noise caused by contaminates. In addition, SB-431542 (Inman et al, 2002), a potent and specific inhibitor of T␤R-I kinase was used as active control.…”

Bu-Shen-Ning-Xin decoction: inhibition of osteoclastogenesis by abrogation of the RANKL-induced NFATc1 and NF-&kappa;B signaling pathways via selective estrogen receptor &alpha;

“…BSNXD consists of drying rehmannia root, common anemarrhena rhizome, bark of the Chinese corktree, barbary wolfberry fruit, Chinese dodder seed, shorthorned epimedium herb, spina date seed, and oriental waterplantain rhizome ( Table 1 ). Although BSNXD has a beneficial action on skeletal mass, 8 the cellular and molecular mechanism mediating this action is not fully understood.…”