The purpose of this study is to establish the role of business incubators and their partnership with other economic, state and scientific institutions in Serbia, based on a survey conducted with incubator managers. State institutions and research institutions, as well as universities, are the main partners of business incubators. This cooperation has become a cornerstone of modern Serbian business. To succeed, businesses need to bind to a number of other companies and businesses such as investors, research institutions, economic organizations, local authorities, economic development organizations, and others. The positive effects of creating cluster networks and business incubators are many. Such clustering creates tangible partnerships between start-ups and international companies that support and promote small and medium-sized businesses in Serbia.