Butane metabolism by butane-grown ‘Pseudomonas butanovora’

Arp, Daniel J.

doi:10.1099/13500872-145-5-1173

Cited by 71 publications

(79 citation statements)

References 22 publications

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Section: Introductionmentioning

confidence: 99%

“…When T. butani-vorans is grown on butane, sBMO oxidizes butane primarily to 1-butanol (.85 % terminal oxidation), which is then further oxidized to butyrate via butyraldehyde (Arp, 1999). Further metabolism of butyrate probably proceeds through butyryl-CoA prior to b-oxidation (Arp, 1999). The metabolic pathway of odd-chain or subterminally oxidized alkanes has not yet been fully characterized in T. butanivorans; however, it is known that the metabolism of odd-and even-chain alkane growth substrates is differentially controlled (Doughty et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Kinetic characterization of the soluble butane monooxygenase from Thauera butanivorans, formerly ‘Pseudomonas butanovora’

et al. 2009

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Soluble butane monooxygenase (sBMO), a three-component di-iron monooxygenase complex expressed by the C 2 -C 9 alkane-utilizing bacterium Thauera butanivorans, was kinetically characterized by measuring substrate specificities for C 1 -C 5 alkanes and product inhibition profiles. sBMO has high sequence homology with soluble methane monooxygenase (sMMO) and shares a similar substrate range, including gaseous and liquid alkanes, aromatics, alkenes and halogenated xenobiotics. Results indicated that butane was the preferred substrate (defined by k cat : K m ratios). Relative rates of oxidation for C 1 -C 5 alkanes differed minimally, implying that substrate specificity is heavily influenced by differences in substrate K m values. The low micromolar K m for linear C 2 -C 5 alkanes and the millimolar K m for methane demonstrate that sBMO is two to three orders of magnitude more specific for physiologically relevant substrates of T. butanivorans. Methanol, the product of methane oxidation and also a substrate itself, was found to have similar K m and k cat values to those of methane. This inability to kinetically discriminate between the C 1 alkane and C 1 alcohol is observed as a steady-state concentration of methanol during the two-step oxidation of methane to formaldehyde by sBMO. Unlike methanol, alcohols with chain length C 2 -C 5 do not compete effectively with their respective alkane substrates. Results from product inhibition experiments suggest that the geometry of the active site is optimized for linear molecules four to five carbons in length and is influenced by the regulatory protein component B (butane monooxygenase regulatory component; BMOB). The data suggest that alkane oxidation by sBMO is highly specialized for the turnover of C 3 -C 5 alkanes and the release of their respective alcohol products. Additionally, sBMO is particularly efficient at preventing methane oxidation during growth on linear alkanes ¢C 2, despite its high sequence homology with sMMO. These results represent, to the best of our knowledge, the first kinetic in vitro characterization of the closest known homologue of sMMO.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Kinetic characterization of the soluble butane monooxygenase from Thauera butanivorans, formerly ‘Pseudomonas butanovora’

et al. 2009

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“…The alkane degradation occurs by sequential oxidations of the terminal methyl group of the hydrocarbon [128]. The first enzyme of the pathway, termed butane monooxygenase (BMO), belongs to the SDIMO family and it shows high similarity to the sMMO [63,131].…”

Section: Butane Monooxygenase (Bmo) In Pseudomonas Butanovoramentioning

confidence: 99%

“…Although a number of bacteria have been isolated that are capable of growth on light nalkanes (ethane, propane and butane), their metabolic pathways have received little attention compared to those of methane and liquid n-alkanes [55,128]. The ability to grow on gaseous n-alkanes has been found in some Pseudomonas strains [129] and many strains belonging to the order Actynomycetales, such as those of the genera Corynebacterium, Nocardia, Mycobacterium, Rhodococcus [55,66,128,130]; these genera possess highly hydrophobic cell surface and express numerous oxygenase enzymes which might generally facilitate their growth and predominance in n-alkane enrichments [55].…”

Section: Sdimo -Short-alkanes Oxidizing Monooxygenasesmentioning

confidence: 99%

“…From a biotechnological perspective, gaseous alkanes are inexpensive carbon sources for microbial and increases the rates of substrate turnover [25,122,123]. Some members of the SDIMOs also contain a fourth subunit comprised of a short polypeptide with a bound Rieske center believed to be involved in electron transfer to the hydroxylase [120,[124][125][126][127].Although a number of bacteria have been isolated that are capable of growth on light nalkanes (ethane, propane and butane), their metabolic pathways have received little attention compared to those of methane and liquid n-alkanes [55,128]. The ability to grow on gaseous n-alkanes has been found in some …”

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Monooxygenases involved in the n-alkanes metabolism by Rhodococcus sp. BCP1: molecular characterization and expression of alkB gene

Cappelletti

Fedi

Honda

et al. 2010

Journal of Biotechnology

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Discussion 107 Summary 113Chapter 5 -Analysis of the alkB gene expression Summary 157Chapter 6 -Proteomic analysis of n-alkanes growth of Rhodococcus sp. BCP1 Chapter 1 -General Introduction PrefaceThe quality of life on Earth is tightly related with the overall quality of the environment [1]. During the last century it was commonly believed that the abundance of land and resources were unlimited and it was rarely acknowledged that the production, use, and disposal of hazardous substances had environmental and health effects [1][2][3]. Owing to this, human activity has deliberately or inadvertently released into waters and soil a variety of toxic compounds as refrigerants, paints, solvents, herbicides and pesticides that can cause considerable environmental pollution and human health problems as a result of their persistence, toxicity, and transformation into hazardous metabolites [4,5]. Very soon, however, it became clear how the carelessness and negligence in handling the industrial wastes and gas emissions had caused dramatic effect on the nature and on human health [1].Nowadays, the concern about the global warming and the climate changing induced the government regulatory agencies to establish procedures for assessing the environmental impact and health hazards of chemicals and for controlling/limiting their utilization.International efforts have been applied to remedy many contaminated sites all over the world, either as a response to the risk of adverse health or environmental effects caused by contamination or to enable the site to be redeveloped for use [5]. In response to public and government concern and because of the intriguing research problems presented, environmental scientists, biologists and chemists have been giving increased attention to identifying and determining the behavior and fate of organic compounds in natural ecosystems [5].Bioremediation procedures offer an economical and effective possibility to degrade or render innocuous toxic pollutants using natural biological activity [1]. By definition, bioremediation implies the use of living organisms, primarily microorganisms, to degrade the Since contaminant compounds are transformed by living organisms through reactions that take place as a part of their metabolic processes, environmental biotechnology focuses on the development of techniques to optimize environmental parameters to allow these metabolic pathways to proceed faster and, therefore, to improve the bio-degradation [1]. As a result, the removal of the contaminated soil can be necessary to let the biodegradation proceed in controlled sites (such as bioreactors). These ex situ approaches provide optimal conditions out one single step of the entire bio-degradation process. Hydrocarbons in the environmentHydrocarbons are energy-rich organic compounds consisting of carbon and hydrogen atoms and they can be saturated (only single C-H bounds) or unsaturated (one ore more double or triple C-H bounds). Alkanes are saturated hydrocarbons with the general formula C n H 2n+2 ; they can b...

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Bacterial Metabolism of n-Alkanes and Ammonia under Oxic, Suboxic and Anoxic Conditions

Berthe‐Corti

Fetzner

2002

Acta Biotechnol.

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Butane metabolism by butane-grown ‘Pseudomonas butanovora’

Cited by 71 publications

References 22 publications

Kinetic characterization of the soluble butane monooxygenase from Thauera butanivorans, formerly ‘Pseudomonas butanovora’

Kinetic characterization of the soluble butane monooxygenase from Thauera butanivorans, formerly ‘Pseudomonas butanovora’

Monooxygenases involved in the n-alkanes metabolism by Rhodococcus sp. BCP1: molecular characterization and expression of alkB gene

Bacterial Metabolism of n-Alkanes and Ammonia under Oxic, Suboxic and Anoxic Conditions

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