Butanol production employing fed-batch fermentation by Clostridium acetobutylicum GX01 using alkali-pretreated sugarcane bagasse hydrolysed by enzymes from Thermoascus aurantiacus QS 7-2-4

Pang, Zhiyong; Wei, Lu; Zhang, Hui; Liang, Zheng-Wu; Liang, Jingjuan; Du, Liangwei; Duan, Chengjie; Feng, Jia‐Xun

doi:10.1016/j.biortech.2016.04.013

Cited by 68 publications

(11 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This indicates acidogenesis and solventogenesis took place twice during the entire fermentation process. The dual acidogenesis in the current study is also supported by Pang et al, 41 who carried out fed-batch fermentation for butanol production using sugar cane baggase by Clostridium acetobutylicum GX01. They observed second acidogenesis after 40 h fermentation, mainly due to rapid assimilation of produced acids into solvents during early stages.…”

Section: Results and Discussionsupporting

confidence: 75%

Role of Trace Elements as Cofactor: An Efficient Strategy toward Enhanced Biobutanol Production

Nimbalkar

Khedkar

Parulekar

et al. 2018

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

Metabolic engineering has the potential to steadily enhance product titers by inducing changes in metabolism. Especially, availability of cofactors plays a crucial role in improving efficacy of product conversion. Hence, the effect of certain trace elements was studied individually or in combinations, to enhance butanol flux during its biological production. Interestingly, nickel chloride (100 mg L–1) and sodium selenite (1 mg L–1) showed a nearly 2-fold increase in solvent titer, achieving 16.13 ± 0.24 and 12.88 ± 0.36 g L–1 total solvents with yields of 0.30 and 0.33 g g–1, respectively. Subsequently, the addition time (screened entities) was optimized (8 h) to further increase solvent production up to 18.17 ± 0.19 and 15.5 ± 0.13 g L–1 by using nickel and selenite, respectively. A significant upsurge in butanol dehydrogenase (BDH) levels was observed, which reflected in improved solvent productions. Additionally, a three-dimensional structure of BDH was also constructed using homology modeling and subsequently docked with substrate, cofactor, and metal ion to investigate proper orientation and molecular interactions.

show abstract

Section: Results and Discussionsupporting

confidence: 75%

Role of Trace Elements as Cofactor: An Efficient Strategy toward Enhanced Biobutanol Production

Nimbalkar

Khedkar

Parulekar

et al. 2018

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

show abstract

“…Cellulose and hemicellulose account for more than 60% of dry weight of sugarcane bagasse and can be converted into fermentable sugars either by acid or enzymatic hydrolysis 106 . To achieve this goal, several research groups and companies worldwide have expended efforts and resources to produce not only ethanol, but also other biofuel molecules such as butanol and isobutanol 107, 108, 109…”

Section: New Technologies For Production Of First and Second-generatimentioning

confidence: 99%

Ethanol production in Brazil: a bridge between science and industry

Lopes

Paulillo

Godoy

et al. 2016

Brazilian Journal of Microbiology

319

174

View full text Add to dashboard Cite

In the last 40 years, several scientific and technological advances in microbiology of the fermentation have greatly contributed to evolution of the ethanol industry in Brazil. These contributions have increased our view and comprehension about fermentations in the first and, more recently, second-generation ethanol. Nowadays, new technologies are available to produce ethanol from sugarcane, corn and other feedstocks, reducing the off-season period. Better control of fermentation conditions can reduce the stress conditions for yeast cells and contamination by bacteria and wild yeasts. There are great research opportunities in production processes of the first-generation ethanol regarding high-value added products, cost reduction and selection of new industrial yeast strains that are more robust and customized for each distillery. New technologies have also focused on the reduction of vinasse volumes by increasing the ethanol concentrations in wine during fermentation. Moreover, conversion of sugarcane biomass into fermentable sugars for second-generation ethanol production is a promising alternative to meet future demands of biofuel production in the country. However, building a bridge between science and industry requires investments in research, development and transfer of new technologies to the industry as well as specialized personnel to deal with new technological challenges.

show abstract

“…(2) constructing a xylose pathway [121]; and (3) improving a cellular tolerance towards inhibitors contained in lignocellulosic hydrolysates [119,122,123]. For example, after alkali pretreatment and enzymatic hydrolysis, C. acetobutylicum can produce 14.17 g/L of butanol with a yield of 0.22 g/g sugars in a fed-batch fermentation from sugarcane bagasse [124]. However, the high cost of pretreatment processing has become the key factor for the economic feasibility of cellulosic ABE fermentation [10].…”

Section: Improvement Of Carbohydrate Utilizationmentioning

confidence: 99%

Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia

Huang

et al. 2020

Biotechnol Biofuels

View full text Add to dashboard Cite

The global energy crisis and limited supply of petroleum fuels have rekindled the interest in utilizing a sustainable biomass to produce biofuel. Butanol, an advanced biofuel, is a superior renewable resource as it has a high energy content and is less hygroscopic than other candidates. At present, the biobutanol route, employing acetone-butanolethanol (ABE) fermentation in Clostridium species, is not economically competitive due to the high cost of feedstocks, low butanol titer, and product inhibition. Based on an analysis of the physiological characteristics of solventogenic clostridia, current advances that enhance ABE fermentation from strain improvement to product separation were systematically reviewed, focusing on: (1) elucidating the metabolic pathway and regulation mechanism of butanol synthesis; (2) enhancing cellular performance and robustness through metabolic engineering, and (3) optimizing the process of ABE fermentation. Finally, perspectives on engineering and exploiting clostridia as cell factories to efficiently produce various chemicals and materials are also discussed.

show abstract

Butanol production employing fed-batch fermentation by Clostridium acetobutylicum GX01 using alkali-pretreated sugarcane bagasse hydrolysed by enzymes from Thermoascus aurantiacus QS 7-2-4

Cited by 68 publications

References 34 publications

Role of Trace Elements as Cofactor: An Efficient Strategy toward Enhanced Biobutanol Production

Role of Trace Elements as Cofactor: An Efficient Strategy toward Enhanced Biobutanol Production

Ethanol production in Brazil: a bridge between science and industry

Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia

Contact Info

Product

Resources

About