Butyl octyl phthalate interacts with estrogen receptor α in MCF‑7 breast cancer cells to promote cancer development

Abstract

Environmental pollution with microplastics and in particular, the leaching of plasticizers is increasingly regarded with concern. The uptake of microplastics by marine animals may lead to plasticizers entering the food chain, which can affect human health. Phthalate compounds are considered to act as endocrine disruptors. The present study aimed to analyze the effects of the three phthalates, benzyl butyl phthalate (BBP), butyl cyclohexyl phthalate (BCP) and butyl octyl phthalate (BOP), on the MCF-7 human breast cancer cell line. For this purpose, molecular docking and microscale thermophoresis were applied, and estrogen receptor α (ERα) activation was measured in ESR1-overexpressing 293 cells. BBP, BCP and BOP bound in silico and in vitro to ERα and activated the receptor. In addition, next-generation sequencing was applied and 15 differentially expressed genes were found following treatment of the MCF-7 cells with BOP: CYP1A1,

Meme Kanseri Epigenetiğinde Biyobelirteçler: Çevresel Faktörlerin Etkisi

“…Ancak, eklenen bileşikler doğrudan kovalent polimerizasyona katılmaz, polimer zincirlerine zayıf olarak bağlanırlar. Bu da ftalatların plastikten sızarak, mikroplastik olarak yutulması ile deniz hayvanları ve ardından insanlara maruziyet ile sonuçlanabilir [74]. Dietilheksil ftalat (DEHP), bağırsaktaki lipazlar ile sonradan emilebilen mono-(2-etilheksil) ftalat'a (MEHP) çevrilir.…”

“…Ancak toksik ve endokrin bozucu etkileri sebebiyle kullanımı kısıtlanmıştır. Günümüzde ise BOP gibi diğer ftalat bileşiklerinin meme kanserindeki risk değerlendirmesi yapılmalıdır [74].…”