Butyrate and propionate: important components of toxic dental plaque extracts

Singer, Robert E.; Buckner, B A

doi:10.1128/iai.32.2.458-463.1981

Cited by 135 publications

(66 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plaque contains both microorganisms, their products, and components of host origin and consequently many biologically-active and toxic components (Hausmann 1974, Daly et al 1980, Raisz et al 1981, Hausmann et al 1975, Hausmann, Nair & Dziak 1982a, Singer & Buckner 1980, Singer & Buckner 1981, Duguid, Al-Makadsi & Cowley i980, Kamen 1981. The role of these components in alveolar bone loss in vivo is obscure.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of bone collagen synthesis by dental plaque extract

Multanen

Paunio

Larjava

1985

J of Periodontal Research

View full text Add to dashboard Cite

Dental plaque extract in low doses inhibited the synthesis of collagen in cultured fetal rat calvaria. Using higher doses of the extract or longer treatment periods the synthesis of noncollagen proteins was also decreased. The denatured extract was as effective as the native one. Although the production of type I collagen in the treated bones was markedly decreased the hydroxylation stage of collagen was not altered. The results suggest that dental plaque extract inhibits the synthesis of collagen by bone cells, which may be one reason for the net loss of collagen of alveolar bone in periodontitis.

show abstract

Section: Discussionmentioning

confidence: 99%

Inhibition of bone collagen synthesis by dental plaque extract

Multanen

Paunio

Larjava

1985

J of Periodontal Research

View full text Add to dashboard Cite

show abstract

“…The byproducts of energy metabolism can alter pH and have cytotoxic effects on host cells (Singer and Buckner 1981;Bradshaw and Marsh 1998;Lamont and Jenkinson 1998). Extensive shifts in end products were seen with the Sg community samples (Hendrickson et al 2012).…”

Section: Amino Acid Fermentationmentioning

confidence: 98%

Proteomics of Streptococcus gordonii within a model developing oral microbial community

et al. 2012

View full text Add to dashboard Cite

BackgroundStreptococcus gordonii is one of several species that can initiate the formation of oral biofilms that develop into the complex multispecies microbial communities referred to as dental plaque. It is in the context of dental plaque that periodontal pathogens such as Porphyromonas gingivalis cause disease. We have previously reported a whole cell quantitative proteomics investigation of P. gingivalis in a model dental plaque community of S. gordonii, P. gingivalis, and Fusobacterium nucleatum. Here we report the adaptation of S. gordonii to the same model.Results1122 S. gordonii proteins were detected in S. gordonii control samples, 915 in communities with F. nucleatum, 849 with P. gingivalis, and 649 with all three organisms. Quantitative comparisons showed extensive proteome changes in association with F. nucleatum or P. gingivalis individually or both P. gingivalis and F. nucleatum together. The changes were species specific, though the P. gingivalis interaction may be dominant, indicated by large differences between the proteomes with F. nucleatum or P. gingivalis but limited changes between communities with P. gingivalis or both P. gingivalis and F. nucleatum. The results were inspected manually and an ontology analysis conducted using DAVID. Extensive changes were seen in nutrition pathways with increases in energy metabolism and changes in the resulting byproducts, while the acid and sugar repressed PTS (phosphoenolpyruvate dependent phosphotransferase system) sugar transport systems showed decreases. These results were seen across all the multispecies samples, though with different profiles according to the partner species. F. nucleatum association decreased proteins for the metabolic end products acetate and ethanol but increased lactate, the primary source of acidity from streptococcal cultures. P. gingivalis containing samples had a reduction in levels of proteins for ethanol and formate but increased proteins for both acetate and lactate production. The communities also showed increases in exopolysaccharide synthesis, amino acid biosynthesis, and oxidative stress protection and decreases in adhesion and transporter proteins.ConclusionThis study showed that S. gordonii demonstrates species specific responses during interactions with F. nucleatum or P. gingivalis. Extensive changes were seen in energy metabolism and byproduct production implicating nutrient transfer as an important community interaction.

show abstract

“…Moreover, crevicular fluid from patients with periodontal disease has been shown to contain propionate and butyrate at concentrations around 10 mM and 2.6 mM, respectively (Niederman et al 1997). Propionate and butyrate have also been shown to produce gingival inflammation in Beagle dogs when applied to the periodontium at concentrations down to 5 mM (Singer and Buckner 1981). Volatile sulfur compounds (VSCs) are a family of gases that are highly toxic to tissues even at quite low concentrations (Ng andTonzetich 1984, Johnson et al 1992).…”

Section: Relationship Between Vocs and Diseases Or Health Conditionsmentioning

confidence: 99%

Microbial volatile compounds in health and disease conditions

Thorn¹,

Greenman²

2012

J. Breath Res.

113

View full text Add to dashboard Cite

Microbial cultures and/or microbial associated diseases often have a characteristic smell. Volatile organic compounds (VOCs) are produced by all microorganisms as part of their normal metabolism. The types and classes of VOC produced is wide, including fatty acids and their derivatives (e.g. hydrocarbons, aliphatic alcohols and ketones), aromatic compounds, nitrogen containing compounds, and volatile sulfur compounds. A diversity of ecological niches exist in the human body which can support a polymicrobial community, with the exact VOC profile of a given anatomical site being dependent on that produced by both the host component and the microbial species present. The detection of VOCs is of interest to various disciplines, hence numerous analytical approaches have been developed to accurately characterize and measure VOCs in the laboratory, often from patient derived samples. Using these technological advancements it is evident that VOCs are indicative of both health and disease states. Many of these techniques are still largely confined to the research laboratory, but it is envisaged that in future bedside 'VOC profiling' will enable rapid characterization of microbial associated disease, providing vital information to healthcare practitioners.

show abstract

Butyrate and propionate: important components of toxic dental plaque extracts

Cited by 135 publications

References 22 publications

Inhibition of bone collagen synthesis by dental plaque extract

Inhibition of bone collagen synthesis by dental plaque extract

Proteomics of Streptococcus gordonii within a model developing oral microbial community

Microbial volatile compounds in health and disease conditions

Contact Info

Product

Resources

About