Butyrate-treatment induces gingival epithelial cell death in a three-dimensional gingival-connective tissue hybrid co-culture system

Kurosawa, Yusuke; Yamaguchi, Hirofumi; Uemichi, Kazuki; Shinozuka, Keiji; Kirihara, Yuki; Tsuda, Hiromasa

doi:10.1016/j.jds.2022.08.034

Cited by 4 publications

(1 citation statement)

References 9 publications

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“…Ca9-22 cells were used as representative gingival epithelial cell [19][20][21] purchased from JCRB Cell Bank (Tokyo, Japan; ID: JCRB0625). Ca9-22 cells were cultured in Dulbecco's modified Eagle's medium (Wako Pure Chemical Industries, Osaka, Japan) containing 10% fetal bovine serum (Biowest, Nuaillé, France), 100 μg/mL penicillin, 100 μg/mL streptomycin, and 2 mM L-glutamine (Wako Pure Chemical Industries).…”

Section: Cell Culturementioning

confidence: 99%

P38α contributes to TNF‐α‐induced IL‐8 production in human gingival cells

et al. 2023

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Tumor necrosis factor‐alpha (TNF‐α) is a major inflammatory cytokine that induces interleukin (IL)‐8 production. Although some studies have reported the involvement of the p38 MAPK signaling pathway in TNF‐α‐induced IL‐8 production, its specific regulatory mechanisms in gingival epithelial cells (GECs) are still poorly understood. In the present study, Ca9‐22 cells were used as representative GECs to investigate the effect of p38 signaling on TNF‐α‐induced IL‐8 production. We found that TNF‐α enhanced IL‐8 production in Ca9‐22 cells by activating the p38 signaling pathway, and one of its isoforms, p38α, played a key role. P38α deletion markedly inhibited TNF‐α‐induced IL‐8 expression in Ca9‐22 cells, while p38α gene rescue could reverse this effect. Further studies revealed that TNF‐α‐induced IL‐8 production was markedly reduced when the threonine 180 and tyrosine 182 p38α phosphorylation sites were targeted for mutagenesis to alanine and phenylalanine, respectively, suggesting their critical role in the process. In conclusion, p38α plays an important role in TNF‐α‐induced IL‐8 production, providing a potential therapeutic target to prevent and treat periodontal disease.

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Section: Cell Culturementioning

confidence: 99%