“…Particularly, several Clostridium species including C. butyricum, C. thermobutyricum , and C. tyrobutyricum can produce butyric acid as the main metabolic product and their potential for industrial production of butyric acid has been extensively studied [ 8 – 11 ]. Recent fermentation process studies for bio-production of butyric acid have focused on C. tyrobutyricum using various substrates, including glucose, xylose [ 12 – 14 ], sucrose [ 15 ], cane molasses [ 16 ], corn meal [ 17 ], Jerusalem artichoke [ 18 ], and brown algae [ 19 ]. Since carbon source accounts for a large proportion of raw material costs, second-generation biorefineries focus on using abundant, cheap, renewable lignocellulosic biomass to produce biofuels and bio-based chemicals, including butyric acid [ 8 , 20 , 21 ].…”