Butyriproducens baijiuensis BJN0003: a potential new member of the family Oscillospiraceae isolated from Chinese Baijiu

Wu, Mengqin; Xu, Youqiang; Dai, Mengqi; Li, Weiwei; Zhang, Chengnan; Fan, Guangsen; Sun, Baoguo

doi:10.1007/s13205-023-03624-w

Cited by 6 publications

(2 citation statements)

References 38 publications

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“…Whole-genome sequencing of B. amyloliquefaciens ZZ7 was analyzed through high throughput sequencing based on the Shanghai Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China), and de novo assembly was performed based on SOAPdenovo ( Li et al, 2010 ). Then, the general genetic information of B. amyloliquefaciens ZZ7 was predicted according to previously reported methods ( Hao et al, 2023 ; Wu M. et al, 2023 ). Finally, Genes from B. amyloliquefaciens ZZ7 were annotated to the NR, SwissProt, COG, GO, and KEGG databases ( Ashburner et al, 2000 ; Bairoch, 2000 ; Kanehisa, 2000 ; Jensen et al, 2007 ; Arthur et al, 2013 ).…”

Section: Methodsmentioning

confidence: 99%

“…The rapid development of systems biology has become an important means to explore the biological characteristics, gene function and metabolic regulation of unknown microorganisms ( Fang et al, 2022 ; Zhang et al, 2023 ), and provide directions for the subsequent molecular genetic modification of microorganisms. For example, Wang et al (2022) identified a Lactobacillus pentosus LTJ12 in MFB, and found that LTJ12 has multiple genes related to alcohol metabolism to enhance its ethanol tolerance and alcohol metabolism through whole genome sequencing and analysis; Liu et al (2022) conducted genomic analysis on a caproic acid producing strain of Rummeliibacillus suwonensis 3B-1 isolated from Luzhou flavor baijiu, and excavated its biosynthesis pathway of caproic acid; Wu M. et al (2023) used the whole gene sequencing technology to identify a new strain Butyriproducens baijiuensis BJN0003 that produced butyric acid isolated from the Chinese baijiu, and analyzed the butyric acid metabolic pathway of BJN0003 from glucose. The same is true for the PLO compounds, however, the metabolic pathway of PLO compounds in the microorganisms are still unclear in the MFB, and there are no relevant research reports.…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive genomic and metabolomic analysis revealed the physiological characteristics and pickle like odor compounds metabolic pathways of Bacillus amyloliquefaciens ZZ7 isolated from fermented grains of Maotai-flavor baijiu

Yang,

Zeng,

Zhou

et al. 2023

Front. Microbiol.

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Pickle like odor (PLO) is one of the main defective flavors of Maotai flavor baijiu (MFB). Understanding and controlling the PLO compounds producing strains not only solves the problem of PLO from the source, but also ensures the high-quality production of MFB. However, the relevant research on PLO compounds producing strains has not been reported in MFB. In this study, we identified a Bacillus amyloliquefaciens ZZ7 with high yield of PLO compounds in the fermented grains of MFB, and measured its physiological characteristics. It produces 627 volatile compounds and 1,507 non-volatile compounds. There are 7 volatile sulfur compounds that cause the PLO, the content of dimethyl disulfide, dimethyl trisulfide, and dimethyl sulfur is relatively high, accounting for 89.43% of the total volatile sulfur compounds. The genome size of B. amyloliquefaciens ZZ7 is 3,902,720 bp with a GC content of 46.09%, and a total of 3,948 protein coding genes were predicted. Moreover, the functional annotation of coding genes and an assessment of the metabolic pathways were performed by genome annotation, showing it has strong ability to transport and metabolize amino acids and carbohydrates. Comprehensive genomic and metabolomic analysis, the metabolic pathway of PLO compounds of B. amyloliquefaciens ZZ7 was revealed, which mainly involves 12 enzymes including sulfate adenylyltransferase, cysteine synthase, cystathionine γ-synthase, etc. This work provides biological information support at both genetic and metabolic levels for the mechanism of B. amyloliquefaciens ZZ7 to synthesize PLO compounds, and provides a direction for the subsequent genetic modification of ZZ7 to solve PLO from the source in the MFB.

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