Butyroyl-arginine as a potent virus inactivation agent

Katsuyama, Yukiko; Yamasaki, Hisashi; Tsujimoto, Kazuko; Koyama, A. Hajime; Ejima, Daisuke; Arakawa, Tsutomu

doi:10.1016/j.ijpharm.2008.05.020

Cited by 23 publications

(106 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously we observed that arginine and arginine derivatives are effective in inactivating a variety of enveloped viruses, although a number of them, such as NDV, are not sensitive to arginine (6). When HHV-2 was incubated with mildly acidic arginine solution, this virus was also sensitive to arginine (Fig.…”

Section: Effect Of Arginine On the Inactivation Of Hhv-2mentioning

confidence: 88%

“…However, it was incapable of inactivating non-enveloped poliovirus (a member of the Picornavirus family). Unexpectedly, one enveloped virus, i.e., Newcastle disease virus (NDV; a member of the Paramyxovirus family), was observed not to be inactivated by these reagents, indicating that not all enveloped viruses were sensitive to arginine (6).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Arginine inactivates human herpesvirus 2 and inhibits genital herpesvirus infection

Ikeda

Yamasaki

Minami

et al. 2012

International Journal of Molecular Medicine

Self Cite

View full text Add to dashboard Cite

Abstract. Arginine, among the amino acids, has demonstrated unique properties, including suppression of protein-protein interactions and virus inactivation. We investigated the effects of arginine on the infectivity of human herpesvirus 2 (HHV-2) and the potential application of arginine as a chemotherapeutic agent against genital herpes. Arginine directly inactivated HHV-2 and characterization of the inactivation demonstrated that 1 M arginine at pH 4.3 inactivated the virus more efficiently compared to 0.1 M citrate or 1 M sodium chloride, indicating that neither acidic pH nor ionic strength alone is sufficient for virus inactivation. The effect of arginine was rapid and concentration-dependent. Although virus inactivation was efficient at an acidic pH, arginine inactivated the virus even at a neutral pH, provided that a higher arginine concentration and prolonged incubation time were used. In addition, arginine suppressed the multiplication of HHV-2 under the conditions at which its effect on cell viability was insignificant. Pilot mouse model studies revealed a marked suppression of death by arginine when the mice were infected with HHV-2 through the vaginal route, followed by an intermittent application of acidic arginine by vaginal instillation.

show abstract

Section: Effect Of Arginine On the Inactivation Of Hhv-2mentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Arginine inactivates human herpesvirus 2 and inhibits genital herpesvirus infection

Ikeda

Yamasaki

Minami

et al. 2012

International Journal of Molecular Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…As to the purity of the compound, although further purification by recrystallization was not successful, the solution showed a maximum ultraviolet absorption at 258 nm as expected from the structure (data not shown) and, as shown in Fig. 1, nuclear magnetic resonance (NMR) chemical shifts were observed exclusively at 4.41 (three methylprotons), 8.79 (two ortho-protons), 8.07 (two meta-protons), 8.54 (a para-proton) and 8.46 ppm (formate proton). These spectroscopic features were consistent with the assigned structure and undeuterated hydrogens of 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt (TSP), an internal standard, and revealed that our NMPF solution did not contain possible contaminated materials except the solvent.…”

Section: Preparation Of N-methyl-pyridinium Formate (Nmpf)mentioning

confidence: 92%

“…The viruses were further diluted to determine the number of infectious viruses in the treated preparation by plaque assay. All of the experiments were conducted in duplicate or triplicate (8,9).…”

Section: Preparation Of N-methyl-pyridinium Formate (Nmpf)mentioning

confidence: 99%

Antiviral effect of pyridinium formate, a novel component of coffee extracts

Koyama¹

2010

Int J Mol Med

View full text Add to dashboard Cite

Abstract. N-methyl-pyridinium formate, a novel component of coffee extracts, inhibited the multiplication of both DNA and RNA viruses. In the presence of the compound, the progeny viral yields of both herpes simplex virus type 1 (HSV-1) and poliovirus in HEp-2 cells and those of influenza virus type A in MDCK cells decreased with increasing concentrations of the compound, although the degree of viral sensitivity to this compound differed. In addition, none of these viruses were directly inactivated by the compound at the concentrations tested. Characterization of the mode of action of this compound against HSV-1 multiplication revealed that it inhibits the viral growth primarily at the initial step of virus multiplication, i.e., within 2 h after the onset of multiplication, although the virus multiplication was affected by the compound throughout the multiplication cycle. In addition, this compound showed a significant cytotoxic effect, although the observed antiviral effect was unlikely to be attributed to the cytotoxic effect.

show abstract

“…Recently Katsuyama et al, (2008) established that Butyroyl-arginine, an arginine derivative, strongly inactivates the enveloped virus, as HSV-1. The authors suggest that the ability of arginine to bind membranes may be responsible for the inactivation of viruses.…”

Section: Some Examples Of Traditional Medicinementioning

confidence: 99%