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Brown, Theodore M.

doi:10.1002/9780470145180.ch132

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Su-8 Microfabrication By Photolithography1

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2007

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“…The support was washed with DMF (7 × 800 µL), followed by THF (7 × 800 µL), and dried under vacuum at room temperature for 4 h. Oligonucleotide Synthesis, Purification, and Analysis. 36,39 A MerMade 192 automatic synthesizer (Bioautomation Inc.) was used for oligonucleotide synthesis according to the manufacturer protocol for a 50 nmol scale synthesis optimized for MerMade CPG columns (standard CPG loaded with 3′-C, 110 µm nominal particle size, 1000 Å pore size, loading 20-60 µmol/g). All reagents were obtained from Link Technologies, Bellshill, Scotland.…”

Section: Su-8 Microfabrication By Photolithographymentioning

confidence: 99%

Multistep Synthesis on SU-8: Combining Microfabrication and Solid-Phase Chemistry on a Single Material

et al. 2007

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SU-8 is an epoxy-novolac resin and a well-established negative photoresist for microfabrication and microengineering. The photopolymerized resist is an extremely highly crosslinked polymer showing outstanding chemical and physical robustness with residual surface epoxy groups amenable for chemical functionalization. In this paper we describe, for the first time, the preparation and surface modification of SU-8 particles shaped as microbars, the attachment of appropriate linkers, and the successful application of these particles to multistep solid-phase synthesis leading to oligonucleotides and peptides attached in an unambiguous manner to the support surface.

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Section: Su-8 Microfabrication By Photolithographymentioning

confidence: 99%