C-banding in the polytene chromosomes of species of the plumosus group (Diptera, Chironomidae) and their experimental hybrids

Michailova, Paraskeva

doi:10.1007/bf00055093

Cited by 9 publications

(8 citation statements)

References 13 publications

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“…hurhipe.r and G. .raliirus) of the genus exhibit greater morphological divergence and distinct chromosomal and genetic differences. Many inversions and duplications in the centromere regions differentiate the chromosomes of these two species and an almost complete lack of synapsis occurs throughout all chromosomes (Michailova 1989). From this one can argue that more mutation has accumulated during the divergence of G. harhipcs and G. salinus than between G. y1aucu.r and G .…”

Section: Banding Pattern Of Hybrid Polytene Chromosomesmentioning

confidence: 94%

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Cytogenetic analysis of a hybrid, Glyptotendipes pallens Mg. ×Glyptotendipes glaucus Mg. (Diptera, Chironomidae): evolutionary considerations

Michailova

2009

Journal of Zoological Systematics and Evolutionary Research

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Cytogenetic analysis of a hybrid, Glyptotendipes pallensMg. x Glyptotendipes glaucus Mg. (Diptera, Chironornidae): evolutionary considerations P. MICHAILOVA AbstractThe karyotype of experimentally obtained hybrids between the two closely related species Glyptotendipes pullens and Gljprotendipes .qlaucus is described. Hybridization was successful in one direction only (G. pallens 9 x G. glaucus J). The polytene chromosomes AB and EF of the hybrid show a more or less intimate pairing throughout their length. In the chromosomes CD in which an inversion occurs the characteristic loop is formed. The homologues of chromosome G are almost completely asynaptic. The localization of centromere heterochromatin was also studied. Centromere heterochromatin as well as intercalary heterochromatin could be observed in all chromosomes. By C banding analyses it could be shown that G. pullens has a telomeric chromosome G while in G. gluucus it is acrocentric. According to karyotype similarity it can be assumed that these two species have quite recently derived from a common ancestor since they still share much of their genomic organization. On the Black Sea coast (southeast part of Bulgaria) a natural hybridization zone between the sympatric species G. pullens and G. glaucus has been detected. The idea that hybridization between the two species might finally proceed to the formation of a new species by hybrid origin and introgression is discussed.

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Section: Banding Pattern Of Hybrid Polytene Chromosomesmentioning

confidence: 94%

“…The classical acet-orcein method was applied to stain salivary gland chromosomes. A differential C-band staining method was used in addition (Michailova 1987). Analysis was carried out in general on 10 hybrid individuals (5 PE + 5 8s) as well as on 10 individuals (5 + 5 53) of G. pullens and on 7 individuals (3 2; + 4 33) of G. gluucus.…”

Section: Methodsmentioning

confidence: 99%

Cytogenetic analysis of a hybrid, Glyptotendipes pallens Mg. ×Glyptotendipes glaucus Mg. (Diptera, Chironomidae): evolutionary considerations

Michailova

2009

Journal of Zoological Systematics and Evolutionary Research

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“…All the nuclei present in a slide were analyzed. C. piger larvae were identified at cytological level by the pattern of their constitutive heterochromatin in the salivary gland polytene chromosomes following Michailova (1987). Generally paired homologous chromosomes are not twisted.…”

Section: Conventional Cytogenetic Methodsmentioning

confidence: 99%

“…C. thummi thummi), which are homosequential in their chromosome banding patterns (Keyl 1965). However, the genome of C. riparius is about 30% larger than the one of C. piger (Keyl 1965), which is attributable in part to different amounts and distribution of constitutive heterochromatin (Keyl 1965;Michailova 1987) and several families of repetitive elements (Schaefer and Schmidt 1981;Schmidt 1984;Hankeln 1990;Hankeln and Schmidt 1990;Hankeln et al 1994;Ross et al 1997). Among them, the so called Alu and Hinf minisatellite DNA clusters are interspersed along the chromosome arms (Hankeln 1990) at fixed locations in all the C. riparius populations so far studied (Bovero et al 2002;Ilkova et al 2007).…”

Section: Introductionmentioning

confidence: 96%

Somatic breakpoints, distribution of repetitive DNA and non-LTR retrotransposon insertion sites in the chromosomes of Chironomus piger Strenzke (Diptera, Chironomidae)

Michailova

Ilkova

Hankeln³

et al. 2008

Genetica

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Structural aberrations, their frequency and distribution as well as distribution of the tandem repetitive minisatellite DNA clusters of Alu and Hinf elements and two retroelements, the LINE NLRCth1 and the SINE CTRT1, were analyzed in the genome of the chironomid C. piger Strenzke larvae from a Bulgarian population. A consistent somatic variability in the structure of the polytene chromosomes was detected, showing that the C. piger genome is more actively rearranging than supposed before. Breakpoints were concentrated in proximal parts of chromosomes significantly more often than in distal parts. By FISH analysis we could detect only one locus containing Alu elements and 38 Hinf cluster loci which appear to be dispersed equally all over the chromosomes. The retrotransposons NLRCth1 and CTRT1 are present only in a few loci, but highly variant among different individuals. The mean number of NLRCth1 sites per individual was 18.4 +/- 2.09 and of CTRT1 was 54.8 +/- 8.42. A third of breakpoint locations were close to or coincide with a locus occupied by a retroelement (either NLRCth1 or CTRT1). Nineteen percent of breakpoints coincided with Hinf repetitive DNA elements. Some breakpoints were identical in the two sibling species C. piger and C. riparius Meigen (syn.: C. thummi thummi) and are considered as conserved hot spots of chromosome breakage.

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“…The species Glyptotendipes salinus differs from Glyptotendipes barbipes by chromosomal rearrangements in the chromosome arms A, C, D and E. Significant difference was also found in the amount and quality of the centromeric C- heterochromatin. Thus, some bands that were in the euchromatic state in Glyptotendipes salinus were in the heterochromatic state in Glyptotendipes barbipes (Michailova 1987b). The amount of centromeric DNA in the Ist, IInd and IIIrd chromosomes differs greatly between the two species (Michailova and Nikolov 1992).…”

Section: Introductionmentioning

confidence: 99%

Karyotype characteristics, larval morphology and chromosomal polymorphism peculiarities of Glyptotendipes salinus Michailova, 1983 (Diptera, Chironomidae) from Tambukan Lake, Central Caucasus

Karmokov

Akkizov

2016

CCG

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Data on the karyotype characteristics, larval morphology and features of chromosomal polymorphism of a population of Glyptotendipes salinus Michailova, 1983 (Diptera, Chironomidae) from Tambukan Lake (on the northern macroslope of the central Caucasus) are presented. It was found that diagnostic larval characters of Glyptotendipes salinus from Caucasus in general are similar to those described in previous studies, but with some significant differences. By some morphological characteristics Caucasian larvae appeared to be closer to Glyptotendipes barbipes than to ones provided for European larvae of Glyptotendipes salinus by Contreras-Lichtenberg (1999). Obtained morphological data make possible to conclude that Caucasian population of Glyptotendipes salinus can be a markedly diverged population of the species, probably even subspecies. In the Caucasian population 12 banding sequences were found: two in arms A, B, C, E, and G, and one in arms D and F. Eight of these are already known for this species, and four, salA2, salB2, salEX, and salG3, are described for the first time. Genetic distances between all the previously studied populations of Glyptotendipes salinus were measured using Nei criteria (1972). The population of the central Caucasus occupies a distinct position on the dendrogram compared with populations from Altai and Kazakhstan. All the obtained morphological and cytogenetic data can indicate the plausible relative isolation and complexity of the Caucasus from the viewpoint of microevolution. More researches are required in other parts of Caucasus and other geographically distant regions for more specific allegations.

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C-banding in the polytene chromosomes of species of the plumosus group (Diptera, Chironomidae) and their experimental hybrids

Cited by 9 publications

References 13 publications

Cytogenetic analysis of a hybrid, Glyptotendipes pallens Mg. ×Glyptotendipes glaucus Mg. (Diptera, Chironomidae): evolutionary considerations

Cytogenetic analysis of a hybrid, Glyptotendipes pallens Mg. ×Glyptotendipes glaucus Mg. (Diptera, Chironomidae): evolutionary considerations

Somatic breakpoints, distribution of repetitive DNA and non-LTR retrotransposon insertion sites in the chromosomes of Chironomus piger Strenzke (Diptera, Chironomidae)

Karyotype characteristics, larval morphology and chromosomal polymorphism peculiarities of Glyptotendipes salinus Michailova, 1983 (Diptera, Chironomidae) from Tambukan Lake, Central Caucasus

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