c-erbB-3 and c-erbB-2 protein expression in node-negative breast carcinoma—an immunocytochemical study

Gasparini, Giampietro; Pozza, F; Bevilacqua, P.; Gullick, William J.; Lemoine, Nicholas R.; Maluta, S.; Palma, Paolo Dalla; Caffo, Orazio; Leonardi, Elena; Boracchi, Patrizia

doi:10.1016/s0959-8049(05)80010-3

Cited by 70 publications

(41 citation statements)

References 28 publications

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“…This finding is consistent with the results of a few studies conducted on the issue (Gasparini et al, 1994;Carol et al, 2002;Polednak, 2003;Sebastian et al, 2004a;2004b;Nimeus-Malmstrom et al, 2010;Song et al, 2011).…”

Section: Discussionsupporting

confidence: 92%

“…Consistent with the results of a large number of studies, our study findings showed that nuclear grade played a prognostic role in node negative patients (Yuan et al, 1992;Gasparini et al, 1994;Carlmagno et al, 1996;Andrulis et al, 1998;Carol et al, 2002;Bekir et al, 2003;Polednak, 2003;Sebastian et al, 2004a;2004b;Gurleyik et al, 2007;Lee et al, 2009;Nimeus-Malmstrom et al, 2010;Song et al, 2011;Clayton et al, 2012). In the current study, the survival rate was obtained as 74.8% in grade three compared to 87.1% in grade one (Table 1), which is in contrast with the study by Ashwini et al (2008).…”

Section: Discussionsupporting

confidence: 90%

“…These findings are in line with the results of a large number of studies (Yuan et al, 1992;Gasparini et al, 1994;Carlmagno et al, 1996;Carol et al, 2002;Gurleyik et al, 2007;Florence et al, 2010), while in contrast with those of Androlis' study (Andrulis et al, 1998). Moreover, Herceptin protein had only a significant effect on survival in lymph node positive patients, which is in agreement with the results of the study by Flourence et al (2010).…”

Section: Discussionsupporting

confidence: 89%

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Vascular Invasion as an Independent Prognostic Factor in Lymph Node Negative Invasive Breast Cancer

Rezaianzadeh¹,

Talei²,

Rajaeefard³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Introduction: Identification of simple and measurable prognostic factors is an important issue in treatment evaluation of breast cancer. The present study was conducted to evaluate the prognostic role of vascular invasion in lymph node negative breast cancer patients. Methods: in a retrospective design, we analyzed the recorded profiles of the 1,640 patients treated in the breast cancer department of Motahari clinic affiliated to Shiraz University of Medical Sciences, Shiraz, Iran, from January 1999 to December 2012. Overall and adjusted survivals were evaluated by the Cox proportional hazard model. All the hypotheses were considered two-sided and a p-value of 0.05 or less was considered as statistically significant. Results: Mean age in lymph node negative and positive patients was 50.0 and 49.8 respectively. In lymph node negative patients, the number of nodes, tumor size, lymphatic invasion, vascular invasion, progesterone receptor, and nuclear grade were significant predictors. In lymph node and lymphatic negative patients, vascular invasion also played a significant prognostic role in the survival which was not evident in lymph node negative patients with lymphatic invasion. Discussion: The results of our large cohort study, with long term follow up and using multivariate Cox proportional model and comparative design showed a significant prognostic role of vascular invasion in early breast cancer patients. Vascular invasion as an independent prognostic factor in lymph node negative invasive breast cancer

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 89%

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Vascular Invasion as an Independent Prognostic Factor in Lymph Node Negative Invasive Breast Cancer

Rezaianzadeh¹,

Talei²,

Rajaeefard³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…Over expression of both c-erbB3 in some breast tumours (Gasparini et al, 1994;Lemoine et al, 1992) and c-erbB4 in some cell lines (Plowman et al, 1993b) has been previously reported. However the frequency of their expression in ER positive and ER negative disease is as yet unknown, as is their e ect (if any) on clinical endocrine response.…”

Section: Introductionmentioning

confidence: 76%

c-erbB3 and c-erbB4 expression is a feature of the endocrine responsive phenotype in clinical breast cancer

et al. 1998

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We examined c-erbB3 and c-erbB4 mRNA expression in 47 primary breast cancer samples by simultaneous RT ± PCR and have investigated correlations between these parameters and the expression of both ER and EGFR mRNA and protein as measured by RT ± PCR and ICA and with Ki67 immunostaining. A direct association was found between c-erbB3 and c-erbB4 mRNA and ER marker status measured by either RT ± PCR (c-erbB3 P=0.0003; c-erbB4 P=0.02) or ICA (c-erbB-3 P=0.002; c-erbB4 P=0.01). Inverse associations were seen between c-erbB3 and c-erbB4 mRNA marker status and EGFR membrane protein (c-erbB3: P=0.003; cerbB4: P=0.003) and mRNA (c-erbB4: P=0.009) status. These associations were reinforced by Spearman Rank Correlation Tests. A signi®cant relationship was seen between Ki67 and c-erbB4 mRNA status and level. Measurements of c-erbB3 protein levels in tumour samples removed from a further 89 patients of known response to endocrine therapy: (i) con®rmed the relationship between c-erbB3 and ER and (ii) identi®ed that patients whose ER positive tumours expressed high levels of c-erbB3 were most likely to bene®t from endocrine measures. A non-signi®cant trend was recorded between c-erbB3 levels and Ki67 immunostaining. These results clearly demonstrate that increased c-erbB3 and c-erbB4 expression appears to be associated with the prognostically-favourable ER phenotype.

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“…Cotransfection of ERBB3 with ERBB2 increases the number of transformation events by an order of magnitude (Alimandi et al, 1995). ErbB3 is also overexpressed in breast cancer (Gasparini et al, 1994), and both genes are upregulated at the level of transcription (Hollywood and Hurst, 1993;Skinner and Hurst, 1993), but unlike ERBB2, overexpression of ERBB3 is not associated with gene ampli®cation (Lemoine et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

An intron 1 enhancer element mediates oestrogen-induced suppression of ERBB2 expression

Bates

Hurst

1997

Oncogene

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Overexpression of the ERBB2 gene in human breast cancer is associated with a poor prognosis and resistance to hormonal treatment and chemotherapy. Oestrogen receptor (ER) positive tumour-derived cell lines are known to express relatively low levels of ERBB2 protein under oestrogenic conditions, but markedly higher levels following withdrawal of oestrogens or administration of tamoxifen. Expression of the closely related ERBB3 gene, which co-operates with ERBB2 in cellular transformation, is now shown to respond to oestrogenic manipulation in a similar way, both responses being mediated largely by transcriptional changes. Six previously undescribed DNase I hypersensitive sites occur within the ®rst intron of ERBB2 in cells that overexpress the gene. A 409 base pair DNA fragment containing one of these sites conferred ER dependent oestrogen inhibition on the ERBB2 promoter in two types of transient transfection assay. DNase I footprinting revealed four separate transcription factor binding sites within this fragment consistent with a role as a transcriptional enhancer. These ®ndings implicate intron 1 sequences in the control of ERBB2 expression for the ®rst time and demonstrate that one site within this region is involved in mediating the transcriptional response to oestrogens. Additionally, there is likely to be synergism between ERBB2 and ERBB3 signalling when both are overexpressed in response to oestrogen inhibition, thereby driving transformed cell behaviour.

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c-erbB-3 and c-erbB-2 protein expression in node-negative breast carcinoma—an immunocytochemical study

Cited by 70 publications

References 28 publications

Vascular Invasion as an Independent Prognostic Factor in Lymph Node Negative Invasive Breast Cancer

Vascular Invasion as an Independent Prognostic Factor in Lymph Node Negative Invasive Breast Cancer

c-erbB3 and c-erbB4 expression is a feature of the endocrine responsive phenotype in clinical breast cancer

An intron 1 enhancer element mediates oestrogen-induced suppression of ERBB2 expression

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