C-fos expression in the rat brain after unilateral labyrinthectomy and its relation to the uncompensated and compensated stages

Cirelli, Chiara; Pompeiano, Maria; D'Ascanio, Paola; Arrighi, Pieranna; Pompeiano, O.

doi:10.1016/0306-4522(95)00369-x

Cited by 97 publications

(50 citation statements)

References 56 publications

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“…Additionally, there is agreement from physiological, pharmacological, and lesion studies that PGO-on neurons in the pontine tegmentum are considered to be the final transferring output components of the PGO generator network [15,60,85]. This is a relevant point, since even dramatic conditions such as labyrinthectomy, that significantly changes the spontaneous firing rate of L/MVN neurons, do not modify c-fos expression in the L/MVN [12], whereas we find that LTPE does.…”

Section: Molecular and Network Implications Of Regional C-fos Expressionsupporting

confidence: 48%

From synapse to gene product: prolonged expression of c-fos induced by a single microinjection of carbachol in the pontomesencephalic tegmentum

Quattrochi

Bazalakova

Hobson

2005

Molecular Brain Research

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It is not known how the brain modifies its regulatory systems in response to the application of a drug, especially over the long term of weeks and months. We have developed a model system approach to this question by manipulating cholinergic cell groups of the laterodorsal and pedunculopontine tegmental (LDT/PPT) nuclei in the pontomesencephalic tegmentum (PMT), which are known to be actively involved in the timing and quantity of rapid eye movement (REM) sleep. In a freely moving feline model, a single microinjection of the cholinergic agonist carbachol conjugated to a latex nanosphere delivery system into the caudolateral PMT elicits a long-term enhancement of one distinguishing phasic event of REM sleep, ponto-geniculo-occipital (PGO) waves, lasting 5 days but without any significant change in REM sleep or other behavioral state. Here, we test the hypothesis that cholinergic activation within the caudolateral PMT alters the postsynaptic excitability of the PGO network, stimulating the prolonged expression of c-fos that underlies this long-term PGO enhancement (LTPE) effect. Using quantitative Fos immunohistochemistry, we found that the number of Fos-immunoreactive (Fos-IR) neurons surrounding the caudolateral PMT injection site decreased sharply by postcarbachol day 03, while the number of Fos-IR neurons in the more rostral LDT/PPT increased >30-fold and remained at a high level following the course of LTPE. These results demonstrate a sustained c-fos expression in response to pharmacological stimulation of the brain and suggest that carbachol's acute effects induce LTPE via cholinergic receptors, with subsequent transsynaptic activation of the LDT/PPT maintaining the LTPE effect.

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Section: Molecular and Network Implications Of Regional C-fos Expressionsupporting

confidence: 48%

From synapse to gene product: prolonged expression of c-fos induced by a single microinjection of carbachol in the pontomesencephalic tegmentum

Quattrochi

Bazalakova

Hobson

2005

Molecular Brain Research

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“…Because UL results in a permanent loss of vestibular inputs from the lesioned side, the compensatory process is assumed to be attributable to the reorganization of the neural network in the central vestibular system (Galiana et al, 1984;Ris et al, 1995). Many brain regions, such as the medial vestibular nucleus (MVN) and inferior olivary complex, are implicated in this process (Kaufman et al, 1992;Cirelli et al, 1996;Balaban and Romero, 1998). However, to date, the molecular mechanisms underlying this process remain unknown.…”

Section: Abstract: Vestibular Compensation; Labyrinthectomy; Lesionimentioning

confidence: 99%

Spatiotemporal Dynamics of Brain-Derived Neurotrophic Factor mRNA Induction in the Vestibulo-Olivary Network during Vestibular Compensation

Li¹,

Hashimoto²,

Tokuyama

et al. 2001

J. Neurosci.

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Vestibular compensation, which is the behavioral recovery from vestibular dysfunction produced by unilateral labyrinthectomy (UL), is attributed to functional and structural reorganization of neural networks in the central vestibular system. To assess the possible contribution of brain-derived neurotrophic factor (BDNF) to this recovery process, we investigated changes in mRNA expression levels in the central vestibular system after UL. We evaluated BDNF mRNA expression levels by quantitative reverse transcription-PCR and in situ hybridization. We found that BDNF mRNA is differentially induced in the medial vestibular nucleus ipsilateral to UL and in the prepositus hypoglossi and inferior olive on the contralateral side. The BDNF mRNA induction lasted for at least 24 hr and returned to the basal expression level within 72 hr after UL. In contrast to BDNF mRNA induction, the expression of an immediate-early gene, c-fos, quickly reached the maximum level at 3 hr and decreased to the basal level within 24 hr after UL. Neither BDNF or c-fos induction was observed in sham-operated animals. The persistent induction of BDNF after UL temporally corresponded to early behavioral manifestations of vestibular compensation. We further found that trkB mRNA was expressed in the central vestibular network at high levels, although its expression levels did not change over time after UL. Because BDNF is implicated in regulating synaptic structure and function, these results provide support for the hypothesis that BDNF is involved in neuronal reorganization that allows vestibular compensation.

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“…It can be considered as a synthesis of best existing techniques to induce labyrinthectomy in rodents (1,3,4,7,10,11,13,14,18,(23)(24)(25) with a novel standardized microsurgical approach to the mastoid bulla, and to the cochlea. Drilling into the cochlea with subsequent suctioning of the fluid and of cellular contents, as well as the ethanol washes, ensure the destruction of the Corti organ and of the stria vascularis.…”

Section: Discussionmentioning

confidence: 99%

“…By carefully widening it, the typical scalar structure of the cochlea can be recognized. Then, the cochlear cavity is washed with saline and its contents aspired repeatedly as suggested by several authors (1,3,4,10,13,14,24,25). Typical cellular debris of the Corti organ will appear in the suction fluid.…”

Section: Methodsmentioning

confidence: 99%

“…Neural plasticity is one key process in potential recovery after stroke (8). Most surgical techniques reported until now include extensive extirpation of the wall of the meatus acusticus externus and structures of middle ear (1,4,7,9,12,15,16,22,25). The specific anatomy of the rodent skull, however, provides an alternative surgical approach through the tympanic bulla (6,10,11,13).…”

Section: Introductionmentioning

confidence: 99%

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A simple standard technique for labyrinthectomy in the rat: A methodical communication with a detailed description of the surgical process

Nádasy

Raffai

Fehér

et al. 2016

Physiology International

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Aims: Labyrinthectomized rats are suitable models to test consequences of vestibular lesion and are widely used to study neural plasticity. We describe a combined microsurgical-chemical technique that can be routinely performed with minimum damage. Methods: Caudal leaflet of the parotis is elevated. The tendinous fascia covering the bulla is opened frontally from the sternomastoid muscle's tendon while sparing facial nerve branches. A 4 mm diameter hole is drilled into the bulla's hind lower lateral wall to open the common (in rodents) mastoid-tympanic cavity. The cochlear crista (promontory) at the lower posterior part of its medial wall is identified as a bony prominence. A 1 mm diameter hole is drilled into its lower part. The perilymphatic/endolymphatic fluids with tissue debris of the Corti organ are suctioned. Ethanol is injected into the hole. Finally, 10 μL of sodium arsenite solution (50 μM/mL) is pumped into the labyrinth and left in place for 15 min. Simple closure in two layers (fascia and skin) is sufficient. Results and conclusion: All rats had neurological symptoms specific for labyrinthectomy (muscle tone, body position, rotatory movements, nystagmus, central deafness). Otherwise, their behavior was unaffected, drinking and eating normally. After a few days, they learned to balance relying on visual and somatic stimuli (neuroplasticity).

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C-fos expression in the rat brain after unilateral labyrinthectomy and its relation to the uncompensated and compensated stages

Cited by 97 publications

References 56 publications

From synapse to gene product: prolonged expression of c-fos induced by a single microinjection of carbachol in the pontomesencephalic tegmentum

From synapse to gene product: prolonged expression of c-fos induced by a single microinjection of carbachol in the pontomesencephalic tegmentum

Spatiotemporal Dynamics of Brain-Derived Neurotrophic Factor mRNA Induction in the Vestibulo-Olivary Network during Vestibular Compensation

A simple standard technique for labyrinthectomy in the rat: A methodical communication with a detailed description of the surgical process

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