c‐kit<sup>&lt;low</sup>Pluripotent Hemopoietic Stem Cells Form CFU‐S on Day 16

Lian, Zhe‐Xiong; Feng, Biao; Sugiura, Kikuya; Inaba, Muneo; Chen, Yú; Jin, Tienan; Fan, Tianxue; Cui, Yunze; Yasumizu, Ryoji; Toki, Junko; Adachi, Yasushi; Hisha, Hiroko; Ikehara, Susumu

doi:10.1002/stem.170039

Cited by 18 publications

(22 citation statements)

References 24 publications

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“…B -F) B Cells BMC were collected and layered onto a NycoPrepe (NycoMed Pharma As, Oslo, Norway) discontinuous density gradient. After centrifugation at 800g for 25 min, cells with a density of 1:066 , p , 1:077 were collected (Lian et al, 1999). The low-density cells were treated with a mixture of rat mAbs against mouse sIgM, CD24 (HSA) and CD19 followed by incubation with anti-rat IgGconjugated magnetic-beads (Dynabeads w ).…”

Section: Immunofluorescence Labeling and Facs Analysismentioning

confidence: 99%

Increased Frequency of Pre–Pro B Cells in the Bone Marrow of New Zealand Black (NZB) Mice: Implications for aDevelopmental Block in B Cell Differentiation

Lian

Kita

Okada

et al. 2002

Journal of Immunology Research

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Reductions in populations of both Pre-B cell (Hardy fractions D) and Pro-B cells (Hardy fractions B–C) have been described in association with murine lupus. Recent studies of B cell populations, based on evaluation of B cell differentiation markers, now allow the enumeration and enrichment of other stage specific precursor cells. In this study we report detailed analysis of the ontogeny of B cell lineage subsets in New Zealand black (NZB) and control strains of mice. Our data suggest that B cell development in NZB mice is partially arrested at the fraction A Pre–Pro B cell stage. This arrest at the Pre-Pro B cell stage is secondary to prolonged lifespan and greater resistance to spontaneous apoptosis. In addition, expression of the gene encoding the critical B cell development transcription factor BSAP is reduced in the Pre–Pro B cell stage in NZB mice. This impairment may influence subsequent B cell development to later stages, and thereby accounts for the down-regulation of the B cell receptor component Igα (mb-1). Furthermore, levels of expression of the Rug2, λ5 and Igβ (B29) genes are also reduced in Pre–Pro B cells of NZB mice. The decreased frequency of precursor B cells in the Pre–Pro B cell population occurs at the most primitive stage of B cell differentiation.

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Section: Immunofluorescence Labeling and Facs Analysismentioning

confidence: 99%

Increased Frequency of Pre–Pro B Cells in the Bone Marrow of New Zealand Black (NZB) Mice: Implications for aDevelopmental Block in B Cell Differentiation

Lian

Kita

Okada

et al. 2002

Journal of Immunology Research

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“…We have recently shown that mouse P-HSCs are c-kit <low by a long-term repopulating assay after serial BMT [29] and CFU-S formation assay [30]. The c-kit <low P-HSCs show the capacity not only to differentiate into multilineage cells but also to self-renew for more than 3.5 years (M. Inaba et al, manuscript in preparation).…”

Section: Effects Of Cytokines On Generation Of Dcs From Hscsmentioning

confidence: 99%

Development of Mouse Dendritic Cells from Lineage‐Negative c‐kit^<lowPluripotent Hemopoietic Stem Cells In Vitro

et al. 2000

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“…21,23 The donor MHC class I expressing cells (H-2K b ) in the recipient marrow included a cellular population that expressed c-Kit þ , Lineage 2 and Sca-1 þ (KLS), which was CD34 2 , and these cells have long-term self-renewal potential and the ability to generate multilineage hematopoietic cells. 24 Despite the presence of these progenitor hematopoietic cells in the marrow, we observed a delay in recovery after transplantation from peripheral blood cytopenias, particularly in the mice that received the highest dose (9 Â 10 6 ) of S-59-treated T-cells. This delay in hematological recovery might have been related to a dual effect of radiation-induced injury to the bone marrow and limited GVHD directed at host marrow stromal cells.…”

Section: Discussionmentioning

confidence: 74%

Improved engraftment with minimal graft-versus-host disease after major histocompatibility complex-mismatched cord blood transplantation with photochemically treated donor lymphocytes

Kanathezhath¹,

Mizokami²,

Stanislaus³

et al. 2011

Exp Biol Med (Maywood)

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There is a significant risk of severe graft-versus-host disease (GVHD) and graft failure after unrelated umbilical cord blood transplantation (CBT) if donor-recipient pairs are mismatched at major histocompatibility complex (MHC) loci. To mitigate these risks after MHC-mismatched CBT, we infused psoralen-treated, photochemically inactivated, mature donor T-lymphocytes with MHC (H2-haplotype) mismatched murine donor fetal near-term peripheral blood (FNPB) cells after sublethal irradiation. We analyzed the rates of donor engraftment, GVHD and long-term survival in H2 haplotype disparate (C57BL/6 [H-2(b)/Thy1.1] → AKR [H-2(k)/Thy1.2]) recipient mice. We observed inconsistent donor engraftment after transplantation with cord blood alone, but superior engraftment and long-term survival after FNPB transplantation supplemented with psoralen-treated donor T-lymphocytes. Additionally, there was fatal GVHD after FNPB co-infusion with untreated donor T-lymphocytes, but minimal GVHD after FNPB supplemented with psoralen-treated donor T-lymphocytes transplantation. Donor MHC(high)/c-Kit(+)/lineage(-)/CD34(-) stem cells were noted in the recipient bone marrow compartment following co-infusion of photochemically inactivated T-cells with FNPB. Despite the non-myeloablative preparation before FNPB infusion, complete hematological recovery was delayed until 50-60 d after transplantation. We observed that co-transplantation of psoralen-treated donor T-lymphocytes with FNPB facilitated durable engraftment of donor hematopoietic stem cells in the marrow and splenic compartments with complete but delayed recovery of all hematopoietic lineages. This CBT model establishes the possibility of ensuring donor engraftment across a MHC barrier without severe GVHD.

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c‐kit^<lowPluripotent Hemopoietic Stem Cells Form CFU‐S on Day 16

Abstract: ABSTRACT

Cited by 18 publications

References 24 publications

Increased Frequency of Pre–Pro B Cells in the Bone Marrow of New Zealand Black (NZB) Mice: Implications for aDevelopmental Block in B Cell Differentiation

Increased Frequency of Pre–Pro B Cells in the Bone Marrow of New Zealand Black (NZB) Mice: Implications for aDevelopmental Block in B Cell Differentiation

Development of Mouse Dendritic Cells from Lineage‐Negative c‐kit^<lowPluripotent Hemopoietic Stem Cells In Vitro

Improved engraftment with minimal graft-versus-host disease after major histocompatibility complex-mismatched cord blood transplantation with photochemically treated donor lymphocytes

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c‐kit<lowPluripotent Hemopoietic Stem Cells Form CFU‐S on Day 16

Abstract: ABSTRACT

Cited by 18 publications

References 24 publications

Increased Frequency of Pre–Pro B Cells in the Bone Marrow of New Zealand Black (NZB) Mice: Implications for aDevelopmental Block in B Cell Differentiation

Increased Frequency of Pre–Pro B Cells in the Bone Marrow of New Zealand Black (NZB) Mice: Implications for aDevelopmental Block in B Cell Differentiation

Development of Mouse Dendritic Cells from Lineage‐Negative c‐kit<lowPluripotent Hemopoietic Stem Cells In Vitro

Improved engraftment with minimal graft-versus-host disease after major histocompatibility complex-mismatched cord blood transplantation with photochemically treated donor lymphocytes

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Product

Resources

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c‐kit^<lowPluripotent Hemopoietic Stem Cells Form CFU‐S on Day 16

Development of Mouse Dendritic Cells from Lineage‐Negative c‐kit^<lowPluripotent Hemopoietic Stem Cells In Vitro