c-Myb acts in parallel and cooperatively with Cebp1 to regulate neutrophil maturation in zebrafish

Jin, Hao; Huang, Zhibin; Chi, Yali; Wu, Mei; Zhou, Riyang; Zhao, Lingfeng; Xu, Jin; Zhen, Fenghua; Lan, Yahui; Li, Li; Zhang, Wenqing; Wen, Zilong; Zhang, Yiyue

doi:10.1182/blood-2015-12-686147

Cited by 29 publications

(35 citation statements)

References 64 publications

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“…In human neutrophils, transcriptional and epigenetic analyses have revealed several TFs that are differentially expressed in the comparisons between different differentiation stages . Preneutrophils and immature neutrophils express high levels of C/EBPα, C/EBPɛ, Gfi1, and Klf5, which have been shown to function to promote the granulocytic lineage choice, and several other TFs like Erg, Myb, which have implicated to associate with neutrophil terminal maturation . Moreoever, TFs like Fosl1, Fosl2, Junb, Bcl6, Klf6, and Irf1 are constitutively expressed across differentiation stages and tend to reach the maximal expression in terminally differentiated neutrophils .…”

Section: Granulocytopoiesismentioning

confidence: 99%

“…48 Preneutrophils and immature neutrophils express high levels of C/EBP , C/EBPɛ, Gfi1, and Klf5, which have been shown to function to promote the granulocytic lineage choice, 11,15,29,49 and several other TFs like Erg, Myb, which have implicated to associate with neutrophil terminal maturation. 50,51 Moreoever, TFs like Fosl1, Fosl2, Junb, Bcl6, Klf6, and Irf1 are constitutively expressed across differentiation stages and tend to reach the maximal expression in terminally differentiated neutrophils. 48 Many of these TFs are zinc-finger proteins or leucine zipper motif-containing factors that play roles during inflammatory and oxidative stress responses.…”

Section: Terminal Differentiationmentioning

confidence: 99%

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Transcriptional regulation of neutrophil differentiation and function during inflammation

Udalova

2020

Journal of Leukocyte Biology

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Neutrophils are the most abundant leukocytes in innate immunity where they elicit powerful effector functions to eliminate invading pathogens and modulate the adaptive as well as the innate immune response. Neutrophil function must be tightly regulated during inflammation and infection to avoid additional tissue damage. Increasing evidence suggests that transcription factors (TFs) function as key regulators to modulate transcriptional output, thereby controlling cell fate decision and the inflammatory responses. However, the molecular mechanisms underlying neutrophil differentiation and function during inflammation remain largely uncharacterized. Here, we provide a comprehensive overview of TFs known to be crucial for neutrophil maturation and in the signaling pathways that control neutrophil differentiation and activation. We also outline how emerging genomic and single‐cell technologies may facilitate further discovery of neutrophil transcriptional regulators.

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Section: Granulocytopoiesismentioning

confidence: 99%

Section: Terminal Differentiationmentioning

confidence: 99%

Transcriptional regulation of neutrophil differentiation and function during inflammation

Udalova

2020

Journal of Leukocyte Biology

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“…These phosphorylated amino acids serve as docking sites to recruit signalling molecules that regulate intracellular signalling networks, such as those mediated by MAPK, PKC and Akt; therefore, EGF is involved in the regulation of various biological functions (Li et al., ). c‐myb, belonged to Myb family, is a transcription factor responsible for controlling expression of a wide array of genes implicated in multiple physiological and pathological processes, like transactivation of cytosolic alanine aminotransferase, differentiation of colon epithelial cells and vascular smooth muscle cells, neutrophil maturation, autophagy and tumorigenesis (Ramsay et al., ; Anemaet et al., ; Lee et al., ; Li et al., ; Jin et al., ; Shikatani et al., ). As transcription factor binding to gene promoter is a major approach to regulate the gene transcription, it is easy to understand that EGF regulating the binding of c‐myb to NaPi‐IIb gene promoter can influence its expression in human intestinal cells.…”

Section: Introductionmentioning

confidence: 99%

Identifying the location of epidermal growth factor‐responsive element involved in the regulation of type IIb sodium‐phosphate cotransporter expression in porcine intestinal epithelial cells

Xing

Tan

et al. 2016

Animal Physiology Nutrition

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Phosphate is an important mineral nutrient for both human and animals in growth and physiological functions; thus, much effort in the past has been made to clarify the mechanisms governing its absorption. Previous studies have found that epidermal growth factor (EGF) inhibits phosphate absorption in human intestinal cells via modulating the interaction of transcriptional factor c-myb with sodium-phosphate cotransporter (NaPi-IIb) gene promoter. This finding provoked our interest in determining the effect of EGF on NaPi-IIb gene expression in intestinal cells of pigs and the location of EGF-responsive element in the gene promoter. Using quantitative PCR, it was observed that EGF significantly reduced NaPi-IIb gene expression in porcine intestinal epithelial IPEC-J2 cells. Transfection with a series of constructs that contain different lengths of the 5'-flanking promoter region of the NaPi-IIb gene manifested that EGF-responsive element is located in the -1200 to -800 region. Further, c-myb was extracted from the cell nucleus of IPEC cells that were exposed to EGF or not via immunoprecipitation. The electrophoretic mobility shift assay showed a specific binding of transcription factor c-myb to labelled probes encompassing DNA sequence from -1092 to -1085 (-TCCAGTTG-). This protein-DNA complex was decreased with cells exposed to EGF and abrogated when c-myb was pre-incubated with excessive unlabelled competitive probes. Results from mutagenesis studies demonstrated that the c-myb-binding site is the EGF-responsive element involved in the regulation of NaPi-IIb expression. Identifying the location of EGF-responsive element contributes to understanding mechanisms underlying EGF down-regulated NaPi-IIb gene expression and provides a foundation for further investigating EGF-regulatory functions in phosphate absorption in pig intestine.

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“…Synthesis of digoxigenin-labelled antisense RNA probes and whole mount in situ hybridization were performed as described previously (Jin et al, 2016;Liu et al, 2017). The probes were listed as follows: rag1, myb, lck, ikaros, foxn1, pu.1, lyz, mfap4, gata1 and βe1.…”

Section: Whole-mount In Situ Hybridizationmentioning

confidence: 99%

Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1

et al. 2018

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T lymphocytes are key cellular components of an acquired immune system and play essential roles in cell-mediated immunity. T cell development occurs in the thymus where 95% of immature thymocytes are eliminated via apoptosis. It is known that mutation of , one of the retinoblastoma 1 (Rb1) target genes, results in a decrease in the number of immature T cells in mice. E2F1, an RB1-interacting protein, has been shown to regulate mature T cell development by interfering with thymocyte apoptosis. However, whether Rb1 regulates thymocyte development still needs to be further investigated. Here, we use a zebrafish model to investigate the role of Rb1 in T cell development. We show that Rb1-deficient fish exhibit a significant reduction in T cell number during early development that it is attributed to the accelerated apoptosis of immature T cells in a caspase-dependent manner. We further show that E2F1 overexpression could mimic the reduced T lymphocytes phenotype of Rb1 mutants, and E2F1 knockdown could rescue the phenotype in Rb1-deficient mutants. Collectively, our data indicate that the Rb1-E2F1-caspase axis is crucial for protecting immature T cells from apoptosis during early T lymphocyte maturation.

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c-Myb acts in parallel and cooperatively with Cebp1 to regulate neutrophil maturation in zebrafish

Abstract: Key Points c-Myb is essential for neutrophil terminal differentiation by targeting granule gene expression. c-Myb and Cebp1 act cooperatively to regulate neutrophil maturation in zebrafish.

Cited by 29 publications

References 64 publications

Transcriptional regulation of neutrophil differentiation and function during inflammation

Transcriptional regulation of neutrophil differentiation and function during inflammation

Identifying the location of epidermal growth factor‐responsive element involved in the regulation of type IIb sodium‐phosphate cotransporter expression in porcine intestinal epithelial cells

Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1

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