C-peptide and zinc delivery to erythrocytes requires the presence of albumin: implications in diabetes explored with a 3D-printed fluidic device

Liu, Yueli; Chen, Chengpeng; Summers, Suzanne; Medawala, Wathsala; Spence, Dana M.

doi:10.1039/c4ib00243a

Cited by 37 publications

(62 citation statements)

References 42 publications

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“…For example, in our laboratory, we do not see any biological effects from C-peptide on erythrocytes unless albumin is present in the buffer. 28 In fact, we do not see any binding of C-peptide to the cells unless albumin is present. An evaluation of the literature will show that most reports involving C-peptide efficacy have either albumin directly added to the buffer, or in cell culture media (typically as added fetal bovine serum), or is present naturally (if an in vivo system).…”

Section: Introductionmentioning

confidence: 66%

C-Peptide replacement therapy in type 1 diabetes: are we in the trough of disillusionment?

et al. 2017

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Type 1 Diabetes is associated with such complications as blindness, kidney failure, and nerve damage. Replacing C-peptide, a hormone normally co-secreted with insulin, has been shown to reduce diabetes-related complications. Interestingly, after nearly 30 years of positive research results, C-peptide is still not being co-administered with insulin to diabetic patients. The following review discusses the potential of C-peptide as an auxilliary replacement therapy and why it's not currently being used as a therapeutic.

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Section: Introductionmentioning

confidence: 66%

C-Peptide replacement therapy in type 1 diabetes: are we in the trough of disillusionment?

et al. 2017

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“…Furthermore, most of the reported PDMS cells-on-a-chip systems contained only one or two cell types. 59 Another important advantage includes the integration of transwell inserts for flexible cell culture on the 3D-printed device. Inserts with pre-cultured cells can be inserted and removed from the fluidic base as needed.…”

Section: Advantageous Features Of 3d-printed Microfluidic Devicesmentioning

confidence: 99%

“…60 Most of currently reported 3D-printed microfluidic devices have channel sizes from hundreds of microns to a few millimeters. 33, 41, 43, 59 This is partially because the printers with the highest resolution usually need supporting materials to fill the void spaces ( i.e., a channel) during the printing process (Figure 5A), which typically needs to be removed manually. 16 Unfortunately, it is almost impossible to remove all the waxy supporting materials from a very small channel.…”

Section: Limitations Of Current 3d-printed Microfluidic Devicesmentioning

confidence: 99%

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3D-printed microfluidic devices: fabrication, advantages and limitations—a mini review

et al. 2016

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A mini-review with 79 references. In this review, the most recent trends in 3D-printed microfluidic devices are discussed. In addition, a focus is given to the fabrication aspects of these devices, with the supplemental information containing detailed instructions for designing a variety of structures including: a microfluidic channel, threads to accommodate commercial fluidic fittings, a flow splitter; a well plate, a mold for PDMS channel casting; and how to combine multiple designs into a single device. The advantages and limitations of 3D-printed microfluidic devices are thoroughly discussed, as are some future directions for the field.

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“…7–9 For example, a fluidic device containing pancreatic β-cells, endothelial cells and erythrocytes was recently reported, which enabled the investigation of cell-cell interactions between the three cell types. 10 …”

Section: Introductionmentioning

confidence: 99%

Use of electrospinning and dynamic air focusing to create three-dimensional cell culture scaffolds in microfluidic devices

Chen

Mehl

Sell

et al. 2016

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Organs-on-a-chip has emerged as a powerful tool for pharmacological and physiological studies. A key part in the construction of such a model is the ability to pattern or culture cells in a biomimetic fashion. Most of the reported cells-on-a-chip models integrate cells on a flat surface, which does not accurately represent the extracellular matrix that they experience in vivo. Electrospinning, a technique used to generate sub-micron diameter polymer fibers, has been used as an in vitro cell culture substrate and for tissue engineering applications. Electrospinning of fibers directly into a fully sealed fluidic channel using a conventional setup has not been possible due to issues of confining the fibers into a discrete network. In this work, a dynamic focusing method was developed, with this approach enabling direct deposition of electrospun fibers into a fully sealed fluidic channel, to act as a matrix for cell culture and subsequent studies under continuous flowing conditions. Scanning electron microscopy of electrospun polycaprolactone fibers shows that this method enables the formation of fibrous layers on the inner wall of a 3D-printed fluidic device (mean fiber size = 1.6 ± 0.6 μm and average pore size = 113 ± 19 μm2). Cells were able to be cultured in this 3D scaffold without the addition of adhesion proteins. Media was pumped through the channel at high flow rates (up to 400 μL/min) during a dynamic cell culture process and both the fibers and the cells were found to be strongly adherent. A PDMS fluidic device was also prepared (from a 3D printed mold) and coated with polycaprolactone fibers. The PDMS device enables optical detection and confocal imaging of cultured cells on the fibers. Finally, macrophages were cultured in the devices to study how the fibrous scaffold can affect cell behavior. It was found that under lipopolysaccharide stimulation, macrophages cultured on PCL fibers inside of a channel secreted significantly more cytokines than those cultured on a thin layer of PCL in a channel or directly on the inner wall of a channel. Overall, this study represents a new approach and technique for in vitro cell studies, where electrospinning can be used to easily and quickly create 3D scaffolds that can improve the culture conditions in microfluidic devices.

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C-peptide and zinc delivery to erythrocytes requires the presence of albumin: implications in diabetes explored with a 3D-printed fluidic device

Cited by 37 publications

References 42 publications

C-Peptide replacement therapy in type 1 diabetes: are we in the trough of disillusionment?

C-Peptide replacement therapy in type 1 diabetes: are we in the trough of disillusionment?

3D-printed microfluidic devices: fabrication, advantages and limitations—a mini review

Use of electrospinning and dynamic air focusing to create three-dimensional cell culture scaffolds in microfluidic devices

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