C, Q and H-banding in the analysis of Y chromosome rearrangements in Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae)

Abstract

In Lucilia cuprina C-banding produces procentric bands on all autosomes and deep staining over most of the X and Y chromosomes which conciderably facilitates the analysis of complex Y chromosome rearrangements. The Y chromosome is generally darkly C-banded throughout while in the X chromosome a pale staining segment is found in the distal portion of the long arm. Modulation of the banding reaction results in 'grey' areas in both X and Y. When C-banding is compared with allocycly it is clear that not all heteropycnotic regions in the sex chromosomes C-band to the same extent. Secondary constrictions in the short arms of both X and Y chromosomes are clearly revealed by C-banding, the X satellite being polymorphic for size.--Q-banding results in a brightly fluorescing band in the short arm of structurally normal Y chromosomes. This band loses its fluorescence in some translocations, probably through a position effect. Hoechst 33258 staining does not produce any brightly fluorescing bands.

Polytene chromosome maps in the Medfly Ceratitis capitata

“…The mitotic karyotype of C. capitata has been described by several authors (Radu et al 1975;Southern 1976;Bedo 1986Bedo , 1987Zapater and Robinson 1986;Zacharopoulou 1987). The mitotic complement consists of one pair of sex result of the unique banding patterns.…”

“…The recent investigations of Bedo (1986Bedo ( , 1987 and Zacharopoulou (1987) have shown that the polytene chromosomes of C. capitata are of sufficient quality to permit cytogenetic analysis. For this analysis, thiconstruction of polytene maps and their correlation to the mitotic chromosomes and linkage groups are needed.…”

Correlation of compound autosome segregation and sex-chromosome disjunction in femaleLucilia cuprina(Wied.) (Diptera: Calliphoridae)

“…As noted earlier, chromosome size is important in determining the pairing relationships of non-exchange elements in D. melanogaster females. Comparison of the X chromosomes and the autosomes in mitotic preparations of L. cuprina (Whitten et al 1975;Bedo, 1980), and the relative sizes of C(5L) and C(5R) elements (Foster et al 1976) suggests that the X chromosomes are similar in size to the CCs. More specifically, the normal X is approximately equivalent in size to C(5L)s, and double the size of C(5R)s. Thus a test of whether size-specificity also operates in L. cuprina should be possible, using altered (i.e.…”

Chromosome studies in nine species of Austrian Symphyla (Myriapoda, Tracheata, Arthropoda)

“…Coverslips were removed by the dry-ice method. An air/heatdrying method (similar to that of Bedo, 1980) was also employed.…”