C‐reactive protein and procalcitonin levels in prostate cancer

İlktaç, Abdullah; Kalkan, Senad; Çalışkan, Selahattin

doi:10.1111/ijcp.13935

Cited by 6 publications

(4 citation statements)

References 15 publications

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“…在前文中讨论的miRNA, 也有工作使用分子探针进行识别, Wanunu等 [66] 率先使用分子探针以及纳米孔对miRNA进行检测. 之后基于链霉亲和素修饰的核酸可以被纳米孔识别出来的原理 [67] , Zahid等 [68]…”

Section: 分子探针直接作为载体unclassified

“…经典的官能化方法就是在纳米颗粒上产生多个结合位点, 这种操作可以在低浓度下有效浓缩目标生物分子, 增大检测到的信号频率. 针对miRNA-21, Wang等 [83] rate vs. applied voltage for ssBio34 (red) and dsBio34 (blue) with MS bound [68] . CRP-aptamer complexes through nanopore [72] .…”

Section: 纳米颗粒作为载体mentioning

confidence: 99%

“…特异性增强技术目标生物分子纳米孔类型检测极限表面官能化 α-thrombin g-thrombin SiN x N/A [39] miRNA-21 Quartz 0.1 pmol/L [42] HAdV SARS-CoV-2 PET 6 pfu/mL [43] 10 4 copies/mL [43] N protein Nanopipette 73.204 pg/mL [47] Neuraminidase ClyA 38 nmol/L [49] SARS-CoV-2 Spike Protein ClyA 2.3 nmol/L [50] Barstar t-FhuA 12.6 nmol/L [51] Streptavidin PET N/A [52] L-His, D-His PET N/A [58] L-Trp PI N/A [59] norepinephrine epinephrine MspA 1 μmol/L [61] ribonucleotide MspA N/A [62] 分子探针 miR155 SiN x 10 nmol/L [68] C-reactive protein SiN x 0.3 ng/μL [72] alpha-1 antitrypsin Tau 381 BACE1 Aerolysin 77.9 fmol/L [73] 6.79 fmol/L [73] 86.4 fmol/L [73] miRNA-21 Glass 5 nmol/L [83] Carcinoembryonic-antigen Quartz 0.01 nmol/L [84] alpha fetoprotein α-HL 1 fmol/L [85] ESAT-6/CFP-10 α-HL 10 amol/L [86] α-Syn Quartz 2.2 pmol/L [93] Thrombin AChE Quartz N/A [65] 物理学报 Acta Phys. Sin.…”

Section: Dna链作为载体mentioning

confidence: 99%

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Nanopore sensing specific enhancement technique for single molecule detection

Pan,

Zhao,

Chen

et al. 2024

Acta Phys. Sin.

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Nanopore sensors have become important tools for analyzing biomarkers, including but not limited to nucleic acids, proteins, and other biomolecules that play important roles in life. Though the nanopores themselves have no selectivity towards target molecules, with the help of the specificity enhancement technology, higher sensitivity of nanopore sensing in respond to the target biomarkers could be achieved. In this work, the basic principles of nanopore sensing are introduced first, then methods of nanopore surface modification as well as the development and application of those selectivity enhancement technologies of nanopore sensing in recent years are reviewed. These enhancement technologies primarily fall into two categories: surface functionalization and molecular probes. Surface functionalization is further categorized based on the types of functional molecules employed, while molecular probes are classified by carrier forms. Finally, the paper discusses several challenges that nanopore sensing continues to encounter and proposes suggestions for its future development.

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Section: 分子探针直接作为载体unclassified

Section: 纳米颗粒作为载体mentioning

confidence: 99%

Section: Dna链作为载体mentioning

confidence: 99%

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Nanopore sensing specific enhancement technique for single molecule detection

Pan,

Zhao,

Chen

et al. 2024

Acta Phys. Sin.

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“…Tracking and precisely determining the CRP level in the human body over time could allow us to evaluate the inflammation progression and tumor aggressiveness and monitor the treatment outcomes. Therefore, CRP is an important biomarker for cancer diagnosis and prognosis. − …”

Section: Introductionmentioning

confidence: 99%

Single-Molecule Identification of the Conformations of Human C-Reactive Protein and Its Aptamer Complex with Solid-State Nanopores

Liang

Zhang

et al. 2022

ACS Appl. Mater. Interfaces

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Human C-reactive protein (CRP) is an established inflammatory biomarker and was proved to be potentially relevant to disease pathology and cancer progression. A large body of methodologies have been reported for CRP analysis, including electrochemical/optical biosensors, aptamer, or antibody-based detection. Although the detection limit is rather low until pg/uL, most of which are time-consuming and relatively expensive, and few of them provided CRP single-molecule information. This work demonstrated the nanopore-based approach for the characterization of CRP conformation under versatile conditions. With an optimized pore of 14 nm in diameter, we achieved the detection limit as low as 0.3 ng/μL, voltage polarity significantly influences the electro-osmotic force and CRP translocation behavior, and the pentameric conformation of CRP may dissociate into pro-inflammatory CRP isoforms and monomeric CRP at bias potential above 300 mV. CRP tends to translocate through nanopores faster along with the increase in pH values, due to more surface charge on both CRP and pore inner wall and stronger electro-osmotic force. The CRP could specifically bind with its aptamer of different concentrations to form complexes, and the complexes exhibited distinguishable nanopore translocation behavior compared with CRP alone. The variation of the molar ratio of aptamer significantly influences the orientation of CRP translocation. The plasma test under physiological conditions displayed the ability of the nanopore system on the CRP identification with a concentration of 3 ng/ μL.

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An umbrella review of meta-analyses on diagnostic accuracy of C-reactive protein

Yang

Cao

et al. 2022

International Journal of Surgery

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C‐reactive protein and procalcitonin levels in prostate cancer

Cited by 6 publications

References 15 publications

Nanopore sensing specific enhancement technique for single molecule detection

Nanopore sensing specific enhancement technique for single molecule detection

Single-Molecule Identification of the Conformations of Human C-Reactive Protein and Its Aptamer Complex with Solid-State Nanopores

An umbrella review of meta-analyses on diagnostic accuracy of C-reactive protein

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