C-Reactive Protein (CRP) and Leptin Receptor in Obesity: Binding of Monomeric CRP to Leptin Receptor

Sudhakar, Manu; Silambanan, Santhi; Chandran, Abhinand S.; Prabhakaran, Athira A; Ramakrishnan, Ramya

doi:10.3389/fimmu.2018.01167

Cited by 56 publications

(48 citation statements)

References 69 publications

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“…Due to the high abundance of MAIT cells in the liver, we speculated that BAs may have a modulatory function on the bacterial activation of these cells. Furthermore, there are reports showing that obesity is associated with alterations in MAIT cell function and distribution in human adults as well as with alterations in inflammatory markers such as C‐reactive protein (CRP), macrophage inflammatory proteins (MIP)‐1α and interleukin (IL)‐8 and the expression of chemokine receptor (CXCR3). Thus, the relationship between BA concentrations, MAIT cell activation and inflammatory parameters with regard to weight was the focus of our present investigation.…”

Section: Introductionmentioning

confidence: 99%

MAIT cell activation in adolescents is impacted by bile acid concentrations and body weight

Mendler

Pierzchalski

Bauer

et al. 2020

Clinical and Experimental Immunology

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Bile acids (BAs) are produced by liver hepatocytes and were recently shown to exert functions additional to their well-known role in lipid digestion. As yet it is not known whether the mucosal-associated invariant T (MAIT) cells, which represent 10-15% of the hepatic T cell population, are affected by BAs. The focus of the present investigation was on the association of BA serum concentration with MAIT cell function and inflammatory parameters as well as on the relationship of these parameters to body weight. Blood samples from 41 normal weight and 41 overweight children of the Lifestyle Immune System Allergy (LISA) study were analyzed with respect to MAIT cell surface and activation markers [CD107a, CD137, CD69, interferon (IFN)-γ, tumor necrosis factor (TNF)-α] after Escherichia coli stimulation, mRNA expression of promyelocytic leukemia zinc finger protein (PLZF) and major histocompatibility complex class I-related gene protein (MR1), the inflammatory markers C-reactive protein (CRP), interleukin (IL)-8 and macrophage inflammatory protein (MIP)-1α as well as the concentrations of 13 conjugated and unconjugated BAs. Higher body weight was associated with reduced MAIT cell activation and expression of natural killer cell marker (NKp80) and chemokine receptor (CXCR3). BA concentrations were positively associated with the inflammatory parameters CRP, IL-8 and MIP-1α, but were negatively associated with the number of activated MAIT cells and the MAIT cell transcription factor PLZF. These relationships were exclusively found with conjugated BAs. BA-mediated inhibition of MAIT cell activation was confirmed in vitro. Thus, conjugated BAs have the capacity to modulate the balance between pro-and anti-inflammatory immune responses.

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Section: Introductionmentioning

confidence: 99%

MAIT cell activation in adolescents is impacted by bile acid concentrations and body weight

Mendler

Pierzchalski

Bauer

et al. 2020

Clinical and Experimental Immunology

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“…CRP is currently a hot spot for studying inflammation and related diseases. [30][31][32] Our results showed that elevated CRP was associated with those groups that received antibiotic compared with the control group.…”

Section: Discussionmentioning

confidence: 52%

Establishment and evaluation of specific antibiotic-induced Inflammatory bowel disease (IBD) model in rats

Tong

Qian

et al. 2020

Preprint

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Background: Physical and chemical methods have been established for rat enteritis model, but antibiotic induction has been relatively rare. This article aims to establish and evaluate rat model of Inflammatory bowel disease (IBD) using antibiotics.Methods: Fourteen SD rats were divided into A-G group according to the dosage and method of antibiotics, among which group A was the control group. The drug was stopped on the 7th day, the modeling period was 1-7 days, and the recovery period was 8-15 days. Half of the animals were dissected on 11 th day and the other animals were dissected on 15 th day . Record the food and water intake, body weight, and fecal weight for 2 hours on different days. Nine intestinal flora were analyzed by bacterial culture and three strains were analyzed by quantitative PCR. TNF-α, IL1-β, IL-6 and CRP in abdominal aorta blood were detected and analyzed. Colon and rectal tissues were pathologically examined for inflammation and scored.Results: Rat weight, food intake, water intake, and two-hour feces were significantly different (P = .04, .016, <. 001, .009). Compared with group A, there were significant differences in 9 kinds of flora in the experimental group (all P <.001). Bacteroides , F aecalibacterium prausnitzii , and Dialister invisus concentrations were analyzed by Quantitative real time polymerase chain reaction (q-PCR) and showed significant differences in groups A, C, and F (p = .033). There were significant differences about TNF-α, IL1-β, IL-6 and CRP between the groups (P = .016, .042, .037, .012). The colonic and rectal pathological inflammation scores of other groups were significantly different from those of the control group (all P <.001).Conclusion : Specific antibiotic-induced IBD model in SD rats is feasible.

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“…analyzed the whole genome sequence database of the Atlantic salmon (Salmo salar) and identi ed ve CRP/serum amyloid P-component (SAP) like molecules, CRP/SAP-1a, CRP/SAP-1b, CRP/SAP-1c, CRP/SAP-2, and CRP/SAP-3 [60]. CRP is a novel topic for studying in ammation and related diseases [61][62][63], being associated with chronic in ammation; thus, here CRP was examined in the abdominal blood of the SD rats. The results showed that elevated CRP was associated with groups receiving antibiotics, as with the discussed in ammatory factors.…”

Section: Discussionmentioning

confidence: 99%

Intestinal flora imbalance induced by antibiotic use in rats

Tong¹,

Qian²,

Li³

et al. 2020

Preprint

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Background In our study, we administered various concentrations of antibiotics to rats, to investigate the changes in the flora of rat feces. Methods Similar Sprague Dawley female rats (n = 84) were divided into A - G groups. The rat feces were collected for microbial analysis. The analytical method used a different culture medium for bacterial cultures and count colonies under a microscope. On the 11th and 15th days, we dissected one rat in each group, taking 5 ml of abdominal aortic blood. TNF-α, IL1-β, IL-6, and C-reactive protein (CRP) were detected by an enzyme-linked immune sorbent assay (ELISA). Results Rats have an average weight of 176.26 g. Between the groups no significant difference was found for starting average weight (p > 0.05) but after experiments, significant differences existed in weight, food intake, water intake, and stool samples within 2 hours (p = 0.04, 0.016, < 0.001, and 0.009, respectively). Significant differences were found between the groups for nine tested microbiotas (p < 0.001). TNF-α,IL1-β༌IL-6༌and CRP were significantly different between all groups (p < 0.001). Conclusions Antibiotics can cause disorder in rat intestinal flora, also causing an inflammatory response in their blood system.

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C-Reactive Protein (CRP) and Leptin Receptor in Obesity: Binding of Monomeric CRP to Leptin Receptor

Cited by 56 publications

References 69 publications

MAIT cell activation in adolescents is impacted by bile acid concentrations and body weight

MAIT cell activation in adolescents is impacted by bile acid concentrations and body weight

Establishment and evaluation of specific antibiotic-induced Inflammatory bowel disease (IBD) model in rats

Intestinal flora imbalance induced by antibiotic use in rats

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