2021
DOI: 10.1101/2021.02.14.431188
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c-Rel is required for IL-33-dependent activation of ILC2s

Abstract: Group 2 innate lymphoid cells (ILC2s) are emerging as important cellular regulators of homeostatic and disease-associated immune processes. The cytokine interleukin-33 (IL-33) promotes ILC2-dependent inflammation and immunity, with IL-33 having been shown to activate NF-κB in a wide variety of cell types. However, it is currently unclear which NF-κB members play an important role in IL-33-dependent ILC2 biology. Here, we identify the NF-κB family member c-Rel as a critical component of the IL-33-dependent acti… Show more

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“…Flow cytometry mLN tissues were mechanically disintegrated and filtered through a 70-μm strainer to form single cell suspensions. Cells were stained using described antibodies (Table S1) as previously described [66]. Data was acquired on a BD LSR Fortessa X20 or LSRIIa., and was analysed using Flowjo (Treestar).…”
Section: T Muris-specific Igg Elisamentioning
confidence: 99%
“…Flow cytometry mLN tissues were mechanically disintegrated and filtered through a 70-μm strainer to form single cell suspensions. Cells were stained using described antibodies (Table S1) as previously described [66]. Data was acquired on a BD LSR Fortessa X20 or LSRIIa., and was analysed using Flowjo (Treestar).…”
Section: T Muris-specific Igg Elisamentioning
confidence: 99%