C<sub>60</sub>@lysozyme: a new photosensitizing agent for photodynamic therapy

Soldà, Alice; Cantelli, Andrea; Giosia, Matteo Di; Montalti, Marco; Zerbetto, Francesco; Rapino, Stefania; Calvaresi, Matteo

doi:10.1039/c7tb00800g

Cited by 38 publications

(44 citation statements)

References 85 publications

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“…[81,82] The lysozyme·C 60 complexp roduced 1 O 2 following visible-light irradiation and showedp hotoinduced cytotoxicity toward HeLa cells. [83] As discussed in Section 2.4, LMIC 60 preparedb yu sing the exchange methodh ad highers olubility ands tability than that prepared by using the premixing method.L MIC 60 prepared throught he two methods had different photoinduced cytotoxicities toward HeLa cells. Cell viability was determined by using aW ST-8 assay (Cell Counting Kit-8: Dojindo Laboratories Co., Kumamoto, Japan).…”

Section: Photoinduced Cytotoxicities Of C 60mentioning

confidence: 98%

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Photodynamic Activity of Fullerene Derivatives Solubilized in Water by Natural‐Product‐Based Solubilizing Agents

Antoku

Sugikawa

Ikeda

2018

Chemistry A European J

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Water-soluble fullerenes prepared by using solubilizing agents based on natural products are promising photosensitizers for photodynamic therapy. Cyclodextrin, β-1,3-glucan, lysozyme, and liposomes can stably solubilize not only C and C , but also some C derivatives in water. To improve the solubilities of fullerenes, specific methods have been developed for each solubilizing agent. Water-soluble C and C exhibit photoinduced cytotoxicity under near-ultraviolet irradiation, but not at wavelengths over 600 nm, which are the appropriate wavelengths for photodynamic therapy. However, dyad complexes of solubilized C derivatives combined with light-harvesting antenna molecules improve the photoinduced cytotoxicities at wavelengths over 600 nm. Furthermore, controlling the fullerene and antenna molecule positions within the solubilizing agents affects the performance of the photosensitizer.

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Section: Photoinduced Cytotoxicities Of C 60mentioning

confidence: 98%

“…reported that lysozyme was able to disperse even pristine C 60 monomolecularly in water with a 1:1 stoichiometry . The lysozyme ⋅ C 60 complex produced 1 O 2 following visible‐light irradiation and showed photoinduced cytotoxicity toward HeLa cells …”

Section: Photoinduced Cytotoxicity Of Fullerenes Incorporated Into Somentioning

confidence: 99%

Photodynamic Activity of Fullerene Derivatives Solubilized in Water by Natural‐Product‐Based Solubilizing Agents

Antoku

Sugikawa

Ikeda

2018

Chemistry A European J

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“…Since fullerenes are ideal electron mediators, C 60 @protein hybrids can lead to new applications of C 60 in electrochemical catalytic systems or in biosensing. [10][11][12] Photoinduced reactive oxygen species (ROS) generation by C 60 @lysozyme hybrids C 60 shows a significant visible light-induced generation of ROS, that can be exploited in photodynamic therapy [76][77][78][79][80][81][82][83][84][85][86][87] or photocatalysis. [88][89][90][91] However, the use of C 60 molecules in water still presents important restrictions in its application due to both the dependency of C 60 properties on the physiological environment and the related aggregation phenomena 92 and C 60 poor solubility.…”

Section: Electrochemical Characterization Of C 60 @Lysozyme Hybridsmentioning

confidence: 99%

“…The high performances of C 60 @lysozyme in the visible light-induced generation of ROS in water suggest the potential use of this hybrid as an agent for photodynamic therapy. [76][77][78][79][80][81][82][83][84][85][86][87] Photogenerated singlet oxygen can be used also in synthetic organic chemistry. Even though singlet oxygen is a short-lived metastable excited state of molecular oxygen, it is a practical reagent for compound oxidation and can form carbon-oxygen and heteroatom-oxygen bonds.…”

Section: Electrochemical Characterization Of C 60 @Lysozyme Hybridsmentioning

confidence: 99%

Proteins as supramolecular hosts for C₆₀: a true solution of C₆₀ in water

et al. 2018

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Hybrid systems have great potential for a wide range of applications in chemistry, physics and materials science. Conjugation of a biosystem to a molecular material can tune the properties of the components or give rise to new properties. As a workhorse, here we take a C60@lysozyme hybrid. We show that lysozyme recognizes and disperses fullerene in water. AFM, cryo-TEM and high resolution X-ray powder diffraction show that the C60 dispersion is monomolecular. The adduct is biocompatible, stable in physiological and technologically-relevant environments, and easy to store. Hybridization with lysozyme preserves the electrochemical properties of C60. EPR spin-trapping experiments show that the C60@lysozyme hybrid produces ROS following both type I and type II mechanisms. Due to the shielding effect of proteins, the adduct generates significant amounts of 1O2 also in aqueous solution. In the case of type I mechanism, the protein residues provide electrons and the hybrid does not require addition of external electron donors. The preparation process and the properties of C60@lysozyme are general and can be expected to be similar to other C60@protein systems. It is envisaged that the properties of the C60@protein hybrids will pave the way for a host of applications in nanomedicine, nanotechnology, and photocatalysis.

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“…The hybrid is well-defined and the fullerene binds selectively in the protein-substrate binding pocket. The protein-based supramolecular adduct preserves the photophysical properties of C60 and allows the exploitation of C60 as a photosensitizer for photodynamic treatments [34].…”

Section: Introductionmentioning

confidence: 99%

C<sub>60</sub> Bioconjugation with Proteins: Towards a Palette of Carriers for All pH Ranges

Giosia¹,

Valle²,

Cantelli³

et al. 2018

Preprint

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The high hydrophobicity of fullerenes and the resulting formation of aggregates in aqueous solutions hamper the possibility of their exploitation in many technological applications. Noncovalent bioconjugation of fullerenes with proteins is an emerging approach for their dispersion in aqueous media. Contrary to covalent functionalization, bioconjugation preserves the physicochemical properties of the carbon nanostructure. The unique photophysical and photochemical properties of fullerenes are then fully accessible for applications in nanomedicine, sensoristic, biocatalysis and materials science fields. And yet, proteins are not universal carriers. Their stability depends on the biological conditions for which they have evolved. Here we present two model systems based on pepsin and trypsin. These proteins have opposite net charge at physiological pH. They recognize and disperse C60 in water. UV-Vis spectroscopy, zeta-potential and atomic force microscopy analysis demonstrates that the hybrids are well dispersed and stable in a wide range of pH’s and ionic strengths. A previously validated modelling approach identifies the protein binding pocket involved in the interaction with C60. Computational predictions, combined with experimental investigations, provide powerful tools to design tailor-made C60@proteins bioconjugates for specific applications.

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C₆₀@lysozyme: a new photosensitizing agent for photodynamic therapy

Abstract: C60@lysozyme showed significant visible light-induced singlet oxygen generation in a physiological environment, indicating the potential of this hybrid as an agent for photodynamic therapy.

Cited by 38 publications

References 85 publications

Photodynamic Activity of Fullerene Derivatives Solubilized in Water by Natural‐Product‐Based Solubilizing Agents

Photodynamic Activity of Fullerene Derivatives Solubilized in Water by Natural‐Product‐Based Solubilizing Agents

Proteins as supramolecular hosts for C₆₀: a true solution of C₆₀ in water

C<sub>60</sub> Bioconjugation with Proteins: Towards a Palette of Carriers for All pH Ranges

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C60@lysozyme: a new photosensitizing agent for photodynamic therapy

Abstract: C60@lysozyme showed significant visible light-induced singlet oxygen generation in a physiological environment, indicating the potential of this hybrid as an agent for photodynamic therapy.

Cited by 38 publications

References 85 publications

Photodynamic Activity of Fullerene Derivatives Solubilized in Water by Natural‐Product‐Based Solubilizing Agents

Photodynamic Activity of Fullerene Derivatives Solubilized in Water by Natural‐Product‐Based Solubilizing Agents

Proteins as supramolecular hosts for C60: a true solution of C60 in water

C<sub>60</sub> Bioconjugation with Proteins: Towards a Palette of Carriers for All pH Ranges

Contact Info

Product

Resources

About

C₆₀@lysozyme: a new photosensitizing agent for photodynamic therapy

Proteins as supramolecular hosts for C₆₀: a true solution of C₆₀ in water