C-terminal Amidation of an Osteocalcin-derived Peptide Promotes Hydroxyapatite Crystallization

Hosseini, Samaneh; Naderi-Manesh, Hossein; Mountassif, Driss; Cerruti, Marta; Vali, Hojatollah; Faghihi, Shahab

doi:10.1074/jbc.m112.422048

Cited by 28 publications

(22 citation statements)

References 51 publications

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“…What's more, this synthetic peptide is enriched in proline, in which the carboxal group enhances its interaction with both calcium and phosphate ions, thus effectively promoting hydroapatite crystallization (Hosseini et al, 2013). Secondly, according to the theoretical pI of the peptide, the peptide has negatively charged surfaces in the remineralization solution (pH 7.0), which is believed to interact efficiently with calcium ions.…”

Section: Discussionmentioning

confidence: 99%

Potential of an amelogenin based peptide in promoting reminerlization of initial enamel caries

Yang

Han

et al. 2015

Archives of Oral Biology

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Section: Discussionmentioning

confidence: 99%

Potential of an amelogenin based peptide in promoting reminerlization of initial enamel caries

Yang

Han

et al. 2015

Archives of Oral Biology

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“…However, the negatively charged ‐COOH in side‐chain of TVH19 was changed to the neutral ‐CONH 2 . Although this change may decrease the growth rate of apatite formation, it was reported to avail to develop c‐axis‐oriented construction, which resembled with the morphology of hydroxyapatite in natural bone tissue (Hosseini et al, ; Li, Lan, et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Recent work shows that AMPs do not trigger microbial resistance and some AMPs possess obvious antimicrobial ability against drug‐resistant bacteria, helping to limit microbial resistance resulting from conventional antibiotics (Ma et al, ). As for remineralization, peptides, such as P11‐4, OSN, and 8DSS, have been reported to promote mineral deposition, repair crystalline structures, and reverse the surface characteristics of early caries lesions (Hosseini et al, ; Kirkham et al, ; Yang et al, ). However, so far, no single peptide can simultaneously exert antimicrobial and remineralizing effects.…”

Section: Introductionmentioning

confidence: 99%

Bifunctional anticaries peptides with antibacterial and remineralizing effects

Wang

et al. 2018

Oral Diseases

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Objectives Initial dental caries often occurs in clinic. Reduction of cariogenic bacteria and promotion of remineralization are effective ways to control them. This study was to develop bifunctional anticaries peptides with antibacterial and remineralizing properties. Methods We designed peptides TDH19, TNH19, and TVH19 and selected one through comparing their minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) against Streptococcus mutans and their reaction on mineralization. Then the bifunction of the selected peptide was studied through: (a) effects on S. mutans biofilm, (b) remineralizing effects on initial lesions and (c) stability in saliva and cytocompatibility to human oral keratinocytes (HOKs). Results TVH19 showed the lowest MIC and MBC and a better mineralizing ability. It inhibited new biofilm formation and reduced the viability of old biofilm (p < 0.05). Treating initial caries with TVH19 led to greater recovery of surface microhardness, shallower lesion depth, and higher mineral content (p < 0.05). No significant differences were observed between TVH19 and NaF samples (p > 0.05). TVH19 was stable in saliva and had little effect on HOKs. Conclusions The novel bifunctional anticaries peptide TVH19 was developed with remarkable antibacterial activity and the potential to enhance remineralization of initial caries.

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“…However, in all of these reports, the needle‐like HA is synthesized at pH ≥ 10, and it is considered that the formation of needle‐like HA is difficult in the neutral oral cavity. However, it has been reported that amino acids constituting proteins control the nucleus formation and crystallization of HA, and by interaction with HA, control the crystal growth in the neutral region (Chang et al, ; Hosseini et al, ; Jahromi et al, ; Larson et al, ; Matsumoto et al, ; Shaw et al, ; Tao et al, ; Zhu et al, ). Accordingly, it is considered that the amino acids in saliva proteins affect the form and structure of HA formed on the teeth.…”

Section: Discussionmentioning

confidence: 99%

“…On the upper side of these needle‐like particles, there were observed whisker‐like HA crystal nanoparticles with a width of several nm and a length of 200 nm, which precipitated and grew in the albumin aqueous solution. It is reported by Hosseini et al () that proteins serve as a template for the nucleus formation and crystallization of HA, and it is considered that the formation of a complex of an acid group of the amino acid in albumin and Ca ion serves as a center for the nucleus formation, and further adsorption of phosphate ion and Ca ion causes formation of a cluster nucleus, whereby whisker‐like HA with a width of several nm is rapidly formed. Thus, it is considered that granular nanoparticles or undefined amorphous substances corresponding to the above A or B were not observed in the model system because of the acceleration of growth period.…”

Section: Discussionmentioning

confidence: 99%

STEM and HRTEM studies of accumulated deposits on human tooth surface

Wakasa

Eshita

Nakanishi

et al. 2017

Microscopy Res & Technique

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The aim of this study was to clarify the fine structure of accumulated deposits on the surface of teeth that are considered to affect the gloss of teeth. The study was carried out using, as specimens, human incisor teeth having gloss, which were extracted from teenage donors and those incapable of showing gloss even by brushing which were extracted from donors in their 50s. Thin longitudinal sections of tooth enamel with accumulated deposits on the surface were prepared by focused ion beam (FIB) milling, and the fine structure was analyzed using a scanning transmission electron microscope (STEM) and a high resolution transmission electron microscope (HRTEM). By FIB, thin longitudinal sections could be prepared from tooth enamel together with organic and inorganic substances accumulated on the surface without artifacts. The accumulated deposits on the surface of teeth having gloss were composed of organic substances. However, it was first revealed by STEM observation that the accumulated solid deposits on the surface of teeth having no gloss had a complicated structure wherein inorganic and organic substances coexisted. It is suggested that the organic substances contain proteins derived from saliva. The inorganic substances were spherical and needle-like hydroxyapatites (HAs). It is considered that amino acids constituting the proteins affected the nucleus formation and the crystal formation of HA. It is considered that the unevenness of the accumulated deposits existing on the surface of tooth enamel having no gloss causes the decrease in gloss of teeth due to diffuse reflection of light.

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C-terminal Amidation of an Osteocalcin-derived Peptide Promotes Hydroxyapatite Crystallization

Cited by 28 publications

References 51 publications

Potential of an amelogenin based peptide in promoting reminerlization of initial enamel caries

Potential of an amelogenin based peptide in promoting reminerlization of initial enamel caries

Bifunctional anticaries peptides with antibacterial and remineralizing effects

STEM and HRTEM studies of accumulated deposits on human tooth surface

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