C-terminal extension of calmodulin-like 3 protein from &amp;lt;italic&amp;gt;Oryza sativa&amp;lt;/italic&amp;gt; L.: interaction with a high mobility group target protein

Chinpongpanich, Aumnart; Phean-o-pas, Srivilai; Thongchuang, Mayura; Liu, Qu; Buaboocha, Teerapong

doi:10.1093/abbs/gmv097

Cited by 13 publications

(15 citation statements)

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“…It is well-recognized that calcium mediated signaling is important for plant responses to a wide variety of stresses including pathogen attack (Dodd et al, 2010;Batistic et al, 2012;DeFalco et al, 2010;Lecourieux et al, 2006). A large number of CaM and CaM-like (CML) proteins have been identi ed in rice genome (Boonburapong and Buaboocha 2007;Chinpongpanich et al, 2015). However, a scanty of reports is available for rice CBPs (Zhang et al, 2012;Shin et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Functional Analysis of Calmodulin Binding Protein 60s in Disease Resistance Responses in Rice (Oryza Sativa L.)

Kumari

Prasad

Nirala

et al. 2021

Preprint

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BackgroundOccurrence and prevention of diseases have a big impact on sustainability of staple food crop like rice. The crosstalk between phytohormones and secondary messengers in host cell during infestation of pathogens play a pivotal role in defence responses. Apart from SA and JA, the role of brassinosteroids (BR) in defence responses in plants is unprecedented. The calcium signatures observed during early infection process modulates the expression of calmodulin and other Ca2+ binding protein followed by their interactions with calmodulin binding protein (CBP), which are pivotal in elucidating defence responses in plant. Numerous CBPs have been identified, which modulated stress responses with the help of CBD and other functional domain. Interestingly, Arabidopsis CBP60 (AtCBP60) family protein, SARD1, was involved in defense responses via SAR. However, no rice CBP60 (OsCBP60) has been identified in relation to pathogen infection yet. ResultsIn present investigation, 15 OsCBP60 genes were identified using BLASTP searchers using AtCBP60s as bait sequences. Expression studies showed that 3 OsCBP60s (OsCBP60_5, OsCBP60_10, and OsCBP60_15) genes were upregulated consistently in all the time point studied in rice seedlings treated with fungal (Magnaporthe oryzae) and bacterial (Xanthomonas oryzae) pathogens. Differential expression of OsCBP60s genes were observed in salicylic acid (SA), epi-brassinosteroid (EBR) and jasmonic acid (JA) treated rice seedlings. Taken together, OsCBP60_5 was found to be upregulated in both pathogens and two phytohormones (SA and EBR) treatment. ConclusionsThe differential expression of OsCBP60s genes under phytohormones and pathogens treatment suggests that these genes might be important targets for increasing biotic stress responses in rice.

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Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Functional Analysis of Calmodulin Binding Protein 60s in Disease Resistance Responses in Rice (Oryza Sativa L.)

Kumari

Prasad

Nirala

et al. 2021

Preprint

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“…Primary screening was carried out using the propagated library according to Chinpongpanich et al [ 119 ]. The 600-μl aliquots of XL1-Bule MRF2 cell suspension were mixed with the equivalent of 25,000 pfu of the amplified library and incubated at 37 °C for 15 min before plating as top agar with the addition of IPTG onto an LB agar plate.…”

Section: Methodsmentioning

confidence: 99%

Downstream components of the calmodulin signaling pathway in the rice salt stress response revealed by transcriptome profiling and target identification

et al. 2018

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BackgroundCalmodulin (CaM) is an important calcium sensor protein that transduces Ca2+ signals in plant stress signaling pathways. A previous study has revealed that transgenic rice over-expressing the calmodulin gene OsCam1–1 (LOC_Os03g20370) is more tolerant to salt stress than wild type. To elucidate the role of OsCam1–1 in the salt stress response mechanism, downstream components of the OsCam1–1-mediated response were identified and investigated by transcriptome profiling and target identification.ResultsTranscriptome profiling of transgenic ‘Khao Dawk Mali 105’ rice over-expressing OsCam1–1 and wild type rice showed that overexpression of OsCam1–1 widely affected the expression of genes involved in several cellular processes under salt stress, including signaling, hormone-mediated regulation, transcription, lipid metabolism, carbohydrate metabolism, secondary metabolism, photosynthesis, glycolysis, tricarboxylic acid (TCA) cycle and glyoxylate cycle. Under salt stress, the photosynthesis rate in the transgenic rice was slightly lower than in wild type, while sucrose and starch contents were higher, suggesting that energy and carbon metabolism were affected by OsCam1–1 overexpression. Additionally, four known and six novel CaM-interacting proteins were identified by cDNA expression library screening with the recombinant OsCaM1. GO terms enriched in their associated proteins that matched those of the differentially expressed genes affected by OsCam1–1 overexpression revealed various downstream cellular processes that could potentially be regulated by OsCaM1 through their actions.ConclusionsThe diverse cellular processes affected by OsCam1–1 overexpression and possessed by the identified CaM1-interacting proteins corroborate the notion that CaM signal transduction pathways compose a complex network of downstream components involved in several cellular processes. These findings suggest that under salt stress, CaM activity elevates metabolic enzymes involved in central energy pathways, which promote or at least maintain the production of energy under the limitation of photosynthesis.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1538-4) contains supplementary material, which is available to authorized users.

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“…OsHMGB is an OsCML3 target protein, and binds to DNA within the nucleus to regulate intracellular Ca 2+ (Aumnart et al, 2015). Several studies have shown that HMGB proteins are localized to the nucleus, are able to bind to specific DNA structures, and are involved in a variety of important biological processes (Grasser et al, 2007;Kang et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of CsHMGB Alleviates Phytotoxicity and Propamocarb Residues in Cucumber

Xin

Luan

et al. 2020

Front. Plant Sci.

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Cucumber (Cucumis sativus L.) is one of the most economically important fruits of the Cucurbitaceae family, therefore consideration of potential pesticide residues in the fruit in the context of cucumber breeding and production programs is important. Propamocarb (a pesticide commonly used to prevent downy mildew) is widely used in cucumber cultivation, but the molecular mechanism underlying the degradation and metabolism of propamocarb in cucumber is not well understood. We screened a candidate CsHMGB gene (CsaV3-5G28190) for response to propamocarb exposure using transcriptome data. The coding region of CsHMGB was 624 bp in length and encoded the conserved HMB-box region. CsHMGB expression differed significantly between the "D0351" genotype, which accumulated low levels of propamocarb, and the "D9320" genotype, which accumulated high levels of propamocarb. CsHMGB expression was positively correlated with propamocarb levels in the cucumber peel. CsHMGB expression was upregulated in the fruit peels of the "D0351" genotype following exposure to propamocarb stress for 3-120 h, but no difference was observed in expression between propamocarb treatment and control for the "D9320" genotype. For the "D0351" genotype, CsHMGB expression was higher in the fruit peels and leaves than that in female flowers; expression was moderate in the stems and fruit pulps, and weak in male flowers and roots. The CsHMGB protein was targeted to the nucleus in Arabidopsis protoplasts and in the epidermis of Nicotiana benthamiana leaves. We measured MDA, O 2 − , and H 2 O 2 levels in cucumber plants and found that they were likely to accumulate reactive oxygen species (ROS) in response to propamocarb stress. Analysis of antioxidant enzyme activity (SOD, POD, CAT, APX, GPX, GST, and GR) and the ascorbate-glutathione (AsA-GSH) system showed that the resistance of the plants was reduced and the levels of propamocarb residue was increased in CsHMGBsilenced plants in response to propamocarb stress. Conversely, overexpression of CsHMGB promoted glutathione-dependent detoxification by AsA-GSH system and improved the antioxidant potential, reduced the accumulation of ROS. Ultimately, the metabolism of propamocarb in cucumber was increased via increase in the wax levels and the stomatal conductance.

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C-terminal extension of calmodulin-like 3 protein from <italic>Oryza sativa</italic> L.: interaction with a high mobility group target protein

Cited by 13 publications

References 58 publications

WITHDRAWN: Functional Analysis of Calmodulin Binding Protein 60s in Disease Resistance Responses in Rice (Oryza Sativa L.)

WITHDRAWN: Functional Analysis of Calmodulin Binding Protein 60s in Disease Resistance Responses in Rice (Oryza Sativa L.)

Downstream components of the calmodulin signaling pathway in the rice salt stress response revealed by transcriptome profiling and target identification

Overexpression of CsHMGB Alleviates Phytotoxicity and Propamocarb Residues in Cucumber

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