C-terminal tagging with eGFP yields new insights into expression of connexin45 but prevents rescue of embryonic lethal connexin45-deficient mice

Kreuzberg, Maria M.; Matern, Gabi; Euwens, Carsten; Höher, Thorsten; Wörsdörfer, Philipp; Willecke, Klaus

doi:10.1016/j.ejcb.2009.04.004

Cited by 15 publications

(12 citation statements)

References 54 publications

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“…Several studies have demonstrated that Cx45 is expressed in working ventricular myocardium and isolated myocytes. 13,[16][17][18][19] Although its protein level in normal, disease-free hearts is very low, the absolute amount of its protein expression had not been established. Furthermore, ultrastructural and biochemical evidence of Cx43/Cx45 heteromeric gap junctions in native ventricular myocardium was lacking.…”

Section: Discussionmentioning

confidence: 99%

“…13,[16][17][18][19][30][31][32][33] We have determined that the levels of Cx45 are 0.3% of those of Cx43, and that cardiacrestricted genetic downregulation or overexpression of Cx45 does not result in any notable deficits in electrical conduction throughout the heart under disease-free conditions. It is possible that downregulation of Cx43 with concomitant upregulation of Cx45 could shift the relative stoichiometry of these two connexins within heteromeric gap junctions in remodeled ischemic or failing hearts.…”

Section: Discussionmentioning

confidence: 99%

“…Expression of Cx45 by adult working ventricular myocytes is limited. 13,16 We have demonstrated that Cx45 is distributed throughout working ventricular myocardium of both adult murine and adult human hearts. 17,18 Indeed, Cx45 is responsible for cell-to-cell coupling in Cx43-null myocytes.…”

Section: O N O T D I S T R I B U T Ementioning

confidence: 90%

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Residual Cx45 and its relationship to Cx43 in murine ventricular myocardium

Bao¹,

Kanter²,

Huang³

et al. 2011

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Residual Cx45 and its relationship to Cx43 in murine ventricular myocardium

Bao¹,

Kanter²,

Huang³

et al. 2011

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“…Bronchial epithelium Primary cultures IHC Human [5] Alveolar epithelium Primary cultures NB Rat [13] Tissue IF Rat [14] Tissue IF, WB Mouse [15] Submucosal Tissue IF Rat [14] Primary cultures NB Rat [13] Tissue ISH Mouse [12] Tissue IF, WB Mouse [15] Alveolar endothelium Tissue IF Mouse [38,40] Myoepithelial gland cells Tissue IF Mouse [4] Tracheal smooth muscle cells Tissue IF Rat [102] Bronchiolar smooth muscle cells Tissue IF Mouse [4,103] Fibroblasts Primary cultures NB, RT-PCR, WB Human [104,105] Cx45 Alveolar epithelium Tissue RT-PCR Mouse [12] Bronchiolar smooth muscle cells Tissue Cx45eGFP Mouse [103] Fibroblasts Primary cultures NB, RT-PCR Human [104] Cx46 Alveolar epithelium Tissue IF, WB Mouse [15] Tissue IF Rat [14] Cx: Connexin; Cx45eGFP: Mice expressing Cx45 fused with enhanced green fluorescent protein; IF: immunofluorescence; IHC: immunohistochemistry; ISH: in situ hybridization; NB: northern blot; RT-PCR: reverse transcription polymerase chain reaction; WB: western blot.…”

Section: Cx32mentioning

confidence: 99%

Connexins as therapeutic targets in lung disease

Losa

Chanson²,

Crespin³

2011

Expert Opinion on Therapeutic Targets

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INTRODUCTION: The lung is a mechanically active system exposed to the external environment and is particularly sensitive to injury and inflammation. Studies have identified intercellular communication pathways that promote proper lung function in response to injury and disease. These pathways involve connexins (Cxs) and gap junctional intercellular communication (GJIC). AREAS COVERED IN THIS REVIEW: The functional expression of Cxs in airway epithelium and vasculature, under normal and pathological conditions, is reviewed. Inhibition of GJIC and/or silencing of Cxs have been shown to modulate the course of disease development. Cx-based channels: i) coordinate ciliary beating and fluid transport to promote clearance of particulates, ii) regulate secretion of pulmonary surfactant, in response to deep inhalation by interconnecting type I and type II alveolar epithelial cells, and iii) are key mediators of pro- and anti-inflammatory signalling by the pulmonary endothelium, in order to modulate leukocyte recruitment from the circulation. EXPERT OPINION: Cx-based channels play several central roles in promoting a regulated inflammatory response and facilitating lung repair, thus enabling the pulmonary epithelium and vasculature to behave as integrated systems. Several pathologies can disrupt the normal communication pathways required for proper lung function, including acute lung injury, asthma, cystic fibrosis, pulmonary fibrosis and cancer

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“…Recently, Cx45 was shown to be expressed in the SVZ and rostral migration stream (RMS) (12) and in neurospheres derived from the SGZ (13), but the function of Cx45 in postnatal neurogenic regions has remained unknown. Here we used Cx45 conditional knockout and overexpression to investigate Cx45 function in postnatal neurogenesis.…”

mentioning

confidence: 99%

Connexin45 modulates the proliferation of transit-amplifying precursor cells in the mouse subventricular zone

Khodosevich

Zuccotti

Kreuzberg

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Connexins have been implicated in the regulation of precursor cell migration and proliferation during embryonic development of the mammalian brain. However, their function in postnatal neurogenesis is unclear. Here we demonstrate that connexin (Cx) 45 is expressed in transit-amplifying cells and neuroblasts in the postnatal subventricular zone (SVZ) and modulated the proliferation of SVZ-derived precursor cells in vivo. Thus, overexpression of Cx45 by retroviral injections increased the proliferation of Mash-1-positive transit-amplifying precursor cells in the SVZ. Conversely, conditional deletion of Cx45 in precursor cells decreased proliferation. Finally, we established that Cx45 positively influences cell cycle reentry via ATP signaling that involves intracellular calcium stores and ERK1/2 signaling.

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C-terminal tagging with eGFP yields new insights into expression of connexin45 but prevents rescue of embryonic lethal connexin45-deficient mice

Cited by 15 publications

References 54 publications

Residual Cx45 and its relationship to Cx43 in murine ventricular myocardium

Residual Cx45 and its relationship to Cx43 in murine ventricular myocardium

Connexins as therapeutic targets in lung disease

Connexin45 modulates the proliferation of transit-amplifying precursor cells in the mouse subventricular zone

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