2005
DOI: 10.1021/bi051278c
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C-Terminal Tyrosine of Ferredoxin−NADP+ Reductase in Hydride Transfer Processes with NAD(P)+/H

Abstract: Ferredoxin-NADP+ reductase (FNR) catalyzes the reduction of NADP+ to NADPH in an overall reversible reaction, showing some differences in the mechanisms between cyanobacterial and higher plant FNRs. During hydride transfer it is proposed that the FNR C-terminal Tyr is displaced by the nicotinamide. Thus, this C-terminal Tyr might be involved not only in modulating the flavin redox properties, as already shown, but also in nicotinamide binding and hydride transfer. FNR variants from the cyanobacterium Anabaena … Show more

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Cited by 53 publications
(117 citation statements)
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“…6 with 3A and 3C). These observations correlated with the changes produced in the FNR spectrum upon NADP + complexation (Tejero et al, 2005). Despite the presence of NADP + barely affected apparent k BC between Fld and FNR (Table 1, Fig.…”
Section: Kinetic Analysis Of Multiple-wavelength Absorption Datasupporting
confidence: 73%
“…6 with 3A and 3C). These observations correlated with the changes produced in the FNR spectrum upon NADP + complexation (Tejero et al, 2005). Despite the presence of NADP + barely affected apparent k BC between Fld and FNR (Table 1, Fig.…”
Section: Kinetic Analysis Of Multiple-wavelength Absorption Datasupporting
confidence: 73%
“…Compared to the plant-type FNRs, an equivalent substitution affected the enzymatic activity differently (Table 3). For Anabaena FNR, the replacement of the C-terminal Tyr by Ser led to a considerable decrease in k cat for the diaphorase activity, 26 but the equivalent replacement of C. tepidum FNR did not affect the kinetic parameters significantly. Interactions of the different FNR variants with NADP + were analyzed by difference spectroscopy (Fig.…”
Section: Resultsmentioning
confidence: 93%
“…Although the peak in the 500-510 nm region in the difference spectra has been reported as an indication of stacking between the NADP + nicotinamide ring and the isoalloxazine ring of FAD in the plant-type FNRs, we did not obtain any evidence of such stacking in the case of TrxRlike FNRs. 26,27 Currently, we can conclude only that NADP + binding modulates the local environment around the FAD molecule.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…4: Schematic representation of electron and proton transfer steps catalyzed by the flavoprotein ferredoxin-NADP + -reductase (protein structure PDB-code 2BSA, structural data from ref. [37]). …”
Section: Ferredoxin-nadp + Reductasementioning
confidence: 99%