C-type cytochromes in the photosynthetic electron transfer pathways in green sulfur bacteria and heliobacteria

Azai, Chihiro; Tsukatani, Yusuke; Itoh, Shigeru; Oh‐oka, Hirozo

doi:10.1007/s11120-009-9521-4

Cited by 25 publications

(23 citation statements)

References 76 publications

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“…The loss of these subunits was unexpected as GsbRC preparations from C. tepidum always contain at least PscA and PscC (He et al 2014 ), which may suggest that sample preparation damaged the GsbRC complex used for cryo-EM. GsbRC complexes from P. aestuarii also lack the PscC subunit (Permentier 2000 ; Vasmel et al 1983 ; Schmidt et al 2000 ), but the cytochrome in P. aestuarii binds very loosely unlike that from C. tepidum , and thus may not be comparable, which is consistent with the profound sequence differences between PscC from the two species (Azai et al 2010 ). Besides the core subunit, PscC is the only other transmembrane subunit associated with the GsbRC.…”

Section: Comparison Of the Gsbrc To Other Rcsmentioning

confidence: 82%

Recent advances in the structural diversity of reaction centers

2021

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Photosynthetic reaction centers (RC) catalyze the conversion of light to chemical energy that supports life on Earth, but they exhibit substantial diversity among different phyla. This is exemplified in a recent structure of the RC from an anoxygenic green sulfur bacterium (GsbRC) which has characteristics that may challenge the canonical view of RC classification. The GsbRC structure is analyzed and compared with other RCs, and the observations reveal important but unstudied research directions that are vital for disentangling RC evolution and diversity. Namely, (1) common themes of electron donation implicate a Ca2+ site whose role is unknown; (2) a previously unidentified lipid molecule with unclear functional significance is involved in the axial ligation of a cofactor in the electron transfer chain; (3) the GsbRC features surprising structural similarities with the distantly-related photosystem II; and (4) a structural basis for energy quenching in the GsbRC can be gleaned that exemplifies the importance of how exposure to oxygen has shaped the evolution of RCs. The analysis highlights these novel avenues of research that are critical for revealing evolutionary relationships that underpin the great diversity observed in extant RCs.

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Section: Comparison Of the Gsbrc To Other Rcsmentioning

confidence: 82%

Recent advances in the structural diversity of reaction centers

2021

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“…After passing through the Cyt bc 1 complex, more H + are produced and more electrons return to RC. 45,46 Fourth, a higher proton gradient contributes to a higher ATP production from ADP by ATPase. 47 The mechanism shows how the biohybrid composite improve the photosynthesis of R. palustris.…”

Section: Resultsmentioning

confidence: 99%

Enhancing the Light Coverage of Photosynthetic Bacteria to Augment Photosynthesis by Conjugated Polymer Nanoparticles

Wang

Gao

et al. 2020

ACS Appl. Bio Mater.

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By coating photosynthetic bacteria of Rhodopseudomonas palustris with conjugated polymers nanoparticles modified with positively charged peptide TAT (CPNs-TAT), a bio-optical hybrid composite of R. palustris/CPNs-TAT has been constructed. R. palustris/CPNs-TAT augments the light coverage of R. palustris to broaden the R. palustris absorption due to excellent light-harvesting properties of CPNs-TAT, especially in the ultraviolet region. It leads to converting ultraviolet light to visible light that could be absorbed by R. palustris, allowing antenna systems around the reaction center (RC) of the photosynthetic membrane to absorb more photons, thus photons are excited and transferred to the RC where the electron–hole separation occurs. Therefore, R. palustris/CPNs-TAT improves adenosine triphosphate (ATP) synthesis by increasing proton gradient, resulting in the enhancement of the photosynthetic activity. This effort combines synthetic light-harvesting materials with photosynthetic bacteria without complicated genetic techniques to obtain the hybrid bio-optical systems for augmenting photosynthesis beyond natural photosynthetic bacteria.

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“…660 : The ∆ cycA mutant retarded its growth in the mid-to-late-exponential growth phase as compared to the wild-type, and the ∆ soxB mutant ceased to grow completely before moving into the mid-exponential phase [5]. Their phenotypes have been attributed to the defection of thiosulfate oxidation caused by the disruption of cycA and soxB genes, respectively [5,29]. Therefore, they would recover active growth behavior if the pDSK5191-derivative plasmids incorporating the relevant genes are maintained stably and work well in the C. tepidum cell.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, pDSK5191- cycA and pDSK5191- soxB , both of which contained homologous flanking regions of 450-bp P pscA and 100-bp terminator, were stably maintained in ∆ cycA and ∆ soxB strains, respectively (Figure 2). Although these strains were obtained by insertional inactivation of the relevant genes [5,29], homologous regions left on a chromosome were too small (less than 500 bp) and their recombination with the cycA and soxB genes on plasmids were never observed. We have not investigated in detail how much the frequency of recombination is dependent on lengths of homologous regions.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression System in Green Sulfur Bacteria by Conjugative Plasmid Transfer

2013

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Gene transfer and expression systems in green sulfur bacteria were established by bacterial conjugation with Escherichia coli. Conjugative plasmid transfer from E. coli S17-1 to a thermophilic green sulfur bacterium, Chlorobaculum tepidum (formerly Chlorobium tepidum) WT2321, was executed with RSF1010-derivative broad-host-range plasmids, named pDSK5191 and pDSK5192, that confer erythromycin and streptomycin/spectinomycin resistance, respectively. The transconjugants harboring these plasmids were reproducibly obtained at a frequency of approximately 10-5 by selection with erythromycin and a combination of streptomycin and spectinomycin, respectively. These plasmids were stably maintained in C. tepidum cells in the presence of these antibiotics. The plasmid transfer to another mesophilic green sulfur bacterium, C. limnaeum (formerly Chlorobium phaeobacteroides) RK-j-1, was also achieved with pDSK5192. The expression plasmid based on pDSK5191 was constructed by incorporating the upstream and downstream regions of the pscAB gene cluster on the C. tepidum genome, since these regions were considered to include a constitutive promoter and a ρ-independent terminator, respectively. Growth defections of the ∆cycA and ∆soxB mutants were completely rescued after introduction of pDSK5191-cycA and -soxB that were designed to express their complementary genes. On the other hand, pDSK5191-6xhis-pscAB, which incorporated the gene cluster of 6xhis-pscA and pscB, produced approximately four times more of the photosynthetic reaction center complex with His-tagged PscA as compared with that expressed in the genome by the conventional natural transformation method. This expression system, based on conjugative plasmid, would be applicable to general molecular biological studies of green sulfur bacteria.

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C-type cytochromes in the photosynthetic electron transfer pathways in green sulfur bacteria and heliobacteria

Cited by 25 publications

References 76 publications

Recent advances in the structural diversity of reaction centers

Recent advances in the structural diversity of reaction centers

Enhancing the Light Coverage of Photosynthetic Bacteria to Augment Photosynthesis by Conjugated Polymer Nanoparticles

Gene Expression System in Green Sulfur Bacteria by Conjugative Plasmid Transfer

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