C-type cytoplasmic male sterility in corn

Sotchenko, V. S.; Горбачева, А. Г.; Kosogorova, N. I.

doi:10.3103/s1068367407020048

Cited by 6 publications

(7 citation statements)

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“…The maternal parent was clearly involved in the partial restoration of the mapping population, supporting previous results with different populations (Vidakovic 1988;Sotchenko et al 2007). It is tempting to speculate that the QTL in bins 2.09, 6.04 and 7.03 were responsible for the partial restoration of B37C.…”

Section: Partial Restoration Is Inherited Like An Oligogenic Traitsupporting

confidence: 71%

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QTL involved in the partial restoration of male fertility of C-type cytoplasmic male sterility in maize

et al. 2011

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Partial restoration of male fertility limits the use of C-type cytoplasmic male sterility (C-CMS) for the production of hybrid seeds in maize. Nevertheless, the genetic basis of the trait is still unknown. Therefore, the aim to this study was to identify genomic regions that govern partial restoration by means of a QTL analysis carried out in an F 2 population (n = 180). This population was derived from the Corn Belt inbred lines B37C and K55. F 2 BC 1 progenies were phenotyped at three locations in Switzerland. Male fertility was rated according to the quality and number of anthers as well as the anthesis-silking interval. A weak effect of environment on the expression of partial restoration was reflected by high heritabilities of all fertility-related traits. Partial restoration was inherited like an oligogenic trait. Three major QTL regions were found consistently across environments in the chromosomal bins 2.09, 3.06 and 7.03. Therefore, a marker-assisted counter-selection of partial restoration is promising. Minor QTL regions were found on chromosomes 3, 4, 5, 6 and 8. A combination of partial restorer alleles at different QTL can lead to full restoration of fertility. The maternal parent was clearly involved in the partial restoration, because the restorer alleles at QTL in bins 2.09, 6.04 and 7.03 originated from B37. The three major QTL regions collocated with other restorer genes of maize, a phenomenon, which seems to be typical for restorer genes. Therefore, a study of the clusters of restorer genes in maize could lead to a better understanding of their evolution and function. In this respect, the long arm of chromosome 2 is particularly interesting, because it harbors restorer genes for the three major CMS systems (C, T and S) of maize.

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Section: Partial Restoration Is Inherited Like An Oligogenic Traitsupporting

confidence: 71%

“…Partial restoration might be governed by multiple factors, which may have an effect in the absence of genes responsible for full restoration (Tracy et al 1991;Has 2002). Complementary interactions between paternal and maternal factors probably play an important role (Gontarovskii 1974;Vidakovic 1988;Sotchenko et al 2007).…”

Section: Introductionmentioning

confidence: 99%

QTL involved in the partial restoration of male fertility of C-type cytoplasmic male sterility in maize

et al. 2011

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“…However, Kohls et al (2011) found that most fertile plants exhibit partial restoration in the (B37C × K55) F 2 population, though the inbred line K55 contains Rf4 . Some studies suggested that the interactions of some factors between male parent and female parent might also contribute to plant cytoplasmic male fertility restoration (Sharma, Singh & Singh, 2005; Sotchenko, Gorbacheva & Kosogorova, 2007). The above studies combining with our results indicated that different parents might have effects on the Rf4 function.…”

Section: Discussionmentioning

confidence: 99%

A preliminary identification ofRf-A619, a novel restorer gene for CMS-C in maize (Zea mays*L.)

Liu

Zhao

et al. 2016

PeerJ

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C-type cytoplasmic male sterility (CMS-C) is widely utilized for hybrid maize seed production. However, genetic mechanisms underlying the fertility restoration are very complicated. At present, there is a divergence on the number of fertility restorer genes in maize inbred line A619 for CMS-C. To further elucidate the restoring mechanism of A619, we used genetic analysis and molecular markers to confirm the restorer genes of maize inbred line A619 for C-type male sterile line C48-2 in this study. Firstly, the fertility segregations of (C48-2 × A619)F2 populations were investigated under three environments during 2013–2015. The segregation ratio of fertile and sterile plants in the F2 population fit to 15:1 via chi-square test and this result suggested that there are two dominant restorer genes in A619 for CMS-C, i.e., Rf4 and a novel gene named Rf*-A619. Next, based on the sequence differences between Rf4 and its recessive allelic rf4, a novel dominant marker F2/R2 was developed and validated to genotyping Rf4 in the F2 population. Through genotypic analysis, we found that there were a certain amount of fertile individuals without Rf4 which accounted for 3/16 in the F2 population via chi-square test at the 0.05 level. These results provided another proof to sustain that the inbred line A619 contains one additional restorer gene for CMS-C fertility restoration except Rf4. At last, we used one SSR marker which is tightly linked with the dominant restorer gene Rf5 to analyze those fertile plants without Rf4 in the F2 population. The PCR amplification results showed that Rf*-A619 is not allelic to Rf5 but a novel restorer gene for CMS-C. These results not only provide a basis for the mapping and characterization of a novel restorer gene but also give a new insight into the mechanism of CMS-C fertility restoration.

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“…Duvick (1956) [30] and Tracy et al (1991) [31] already assumed that cool and humid conditions cooperate in restoration of fertility, whereas hot and dry conditions maintain sterility. Hot and dry weather causes the development of fewer anthers and pollen can lose its viability or is non-competitive compared with pollen of fertile plants [32].…”

Section: Discussionmentioning

confidence: 99%

Influence of Soil Moisture and Air Temperature on the Stability of Cytoplasmic Male Sterility (CMS) in Maize (<i>Zea mays</i> L.)

Bueckmann¹,

Thiele²,

Schiemann³

2016

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Cytoplasmic male sterility (CMS) is a maternally inherited trait that suppresses the production of viable pollen. CMS is a useful biological tool for confinement strategies to facilitate coexistence of genetically modified (GM) and non-GM crops in case where it is required. The trait is reversible and can be restored to fertility in the presence of nuclear restorer genes (Rf genes) and by environmental impacts. The aim of this study was to investigate the influence of the level of irrigation on the stability of CMS maize hybrids under defined greenhouse conditions. Additionally the combination of irrigation and air temperature was studied. Three CMS maize hybrids were grown with different levels of irrigation and in different temperature regimes. Tassel characteristics, pollen production and fertility were assessed. The CMS stability was high in hot air temperatures and decreased in lower temperatures. The level of irrigation had no major effect on the level of sterility. The extent of these phenomena was depending on the genotype of CMS maize and should be known before using CMS for coexistence purposes.

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C-type cytoplasmic male sterility in corn

Cited by 6 publications

References 2 publications

QTL involved in the partial restoration of male fertility of C-type cytoplasmic male sterility in maize

QTL involved in the partial restoration of male fertility of C-type cytoplasmic male sterility in maize

A preliminary identification ofRf-A619, a novel restorer gene for CMS-C in maize (Zea mays*L.)

Influence of Soil Moisture and Air Temperature on the Stability of Cytoplasmic Male Sterility (CMS) in Maize (<i>Zea mays</i> L.)

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C-type cytoplasmic male sterility in corn

Cited by 6 publications

References 2 publications

QTL involved in the partial restoration of male fertility of C-type cytoplasmic male sterility in maize

QTL involved in the partial restoration of male fertility of C-type cytoplasmic male sterility in maize

A preliminary identification ofRf*-A619, a novel restorer gene for CMS-C in maize (Zea maysL.)

Influence of Soil Moisture and Air Temperature on the Stability of Cytoplasmic Male Sterility (CMS) in Maize (&lt;i&gt;Zea mays&lt;/i&gt; L.)

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A preliminary identification ofRf-A619, a novel restorer gene for CMS-C in maize (Zea mays*L.)

Influence of Soil Moisture and Air Temperature on the Stability of Cytoplasmic Male Sterility (CMS) in Maize (<i>Zea mays</i> L.)