C-Type Lectin Receptor (CLR)–Fc Fusion Proteins As Tools to Screen for Novel CLR/Bacteria Interactions: An Exemplary Study on Preselected Campylobacter jejuni Isolates

Mayer, Sabine; Moeller, Rebecca; Monteiro, João Tiago Silva; Ellrott, Kerstin; Josenhans, Christine; Lepenies, Bernd

doi:10.3389/fimmu.2018.00213

Cited by 41 publications

(48 citation statements)

References 62 publications

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“…Studying these interactions on cells and tissues is achieved through increasing multivalency and avidity. Fc chimeras of glycan-binding proteins, including Siglecs 31 and C-type lectins 32 , have been widely used. The dimeric nature of Fc chimeras is sufficient, in some cases, to achieve binding, but further increasing multivalency is expected to be advantageous.…”

Section: Discussionmentioning

confidence: 99%

A versatile soluble siglec scaffold for sensitive and quantitative detection of glycan ligands

et al. 2020

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Sialic acid-binding immunoglobulin-type lectins (Siglecs) are immunomodulatory receptors that are regulated by their glycan ligands. The connections between Siglecs and human disease motivate improved methods to detect Siglec ligands. Here, we describe a new versatile set of Siglec-Fc proteins for glycan ligand detection. Enhanced sensitivity and selectivity are enabled through multimerization and avoiding Fc receptors, respectively. Using these Siglec-Fc proteins, Siglec ligands are systematically profiled on healthy and cancerous cells and tissues, revealing many unique patterns. Additional features enable the production of small, homogenous Siglec fragments and development of a quantitative ligand-binding mass spectrometry assay. Using this assay, the ligand specificities of several Siglecs are clarified. For CD33 (Siglec-3), we demonstrate that it recognizes both α2-3 and α2-6 sialosides in solution and on cells, which has implications for its link to Alzheimer’s disease susceptibility. These soluble Siglecs reveal the abundance of their glycan ligands on host cells as self-associated molecular patterns.

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Section: Discussionmentioning

confidence: 99%

A versatile soluble siglec scaffold for sensitive and quantitative detection of glycan ligands

et al. 2020

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“…Whether or not bacterial EPS activate or inhibit innate immunity via the transmembrane CLRs is not yet understood but recent progress in developing CLR-Fc fusion proteins may be useful to screen for EPS/CLR interactions in the future (Mayer et al, 2018 ).…”

Section: Eps Interactions With the Intestinal Mucosamentioning

confidence: 99%

Interactions of Surface Exopolysaccharides From Bifidobacterium and Lactobacillus Within the Intestinal Environment

et al. 2018

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Exopolysaccharides (EPS) are surface carbohydrate polymers present in most bacteria acting as a protective surface layer but also interacting with the surrounding environment. This review discusses the roles of EPS synthesized by strains of Lactobacillus and Bifidobacterium, many of them with probiotic characteristics, in the intestinal environment. Current knowledge on genetics and biosynthesis pathways of EPS in lactic acid bacteria and bifidobacteria, as well as the development of genetic tools, has created possibilities to elucidate the interplay between EPS and host intestinal mucosa. These include the microbiota that inhabits this ecological niche and the host cells. Several carbohydrate recognition receptors located in the intestinal epithelium could be involved in the interaction with bacterial EPS and modulation of immune response; however, little is known about the receptors recognizing EPS from lactobacilli or bifidobacteria and the triggered response. On the contrary, it has been clearly demonstrated that EPS play a relevant role in the persistence of the producing bacteria in the intestinal tract. Indeed, some authors postulate that some of the beneficial actions of EPS-producing probiotics could be related to the formation of a biofilm layer protecting the host against injury, for example by pathogens or their toxins. Nevertheless, the in vivo formation of biofilms by probiotics has not been proved to date. Finally, EPS produced by probiotic strains are also able to interact with the intestinal microbiota that populates the gut. In fact, some of these polymers can be used as carbohydrate fermentable source by some gut commensals thus being putatively involved in the release of bacterial metabolites that exert positive benefits for the host. In spite of the increasing knowledge about the role that these surface molecules play in the interaction of probiotic bacteria with the gut mucosal actors, both intestinal receptors and microbiota, the challenging issue is to demonstrate the functionality of EPS in vivo, which will open an avenue of opportunities for the application of EPS-producing probiotics to improve health.

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“…The general procedure for the production of human and mouse CLR-Fc fusion protein library has been described previously 24 , 42 . The following primers ( Table 1 ) were used for PCR amplification of cDNA fragments encoding the extracellular part of the respective C-type lectin receptor (CLR).…”

Section: Methodsmentioning

confidence: 99%

ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences

Shanthamurthy

Jain

Yehuda

et al. 2018

Sci Rep

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Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses. Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray. The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca2+-dependent fashion.

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C-Type Lectin Receptor (CLR)–Fc Fusion Proteins As Tools to Screen for Novel CLR/Bacteria Interactions: An Exemplary Study on Preselected Campylobacter jejuni Isolates

Cited by 41 publications

References 62 publications

A versatile soluble siglec scaffold for sensitive and quantitative detection of glycan ligands

A versatile soluble siglec scaffold for sensitive and quantitative detection of glycan ligands

Interactions of Surface Exopolysaccharides From Bifidobacterium and Lactobacillus Within the Intestinal Environment

ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences

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