C-type virus antigens detected by immuno-fluorescence in human bone tumour cultures

Zürcher, C.; Brinkhof, J.; Bentvelzen, P.; Man, J. C. H. de

doi:10.1038/254457a0

Cited by 32 publications

(7 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alterna-tively, the different classes may result from different etiologic agents, each of which produces a characteristic pattern. In mice, there is some evidence that a C-type virus can produce osteosarcoma (Finkel et al, 1966); there is also some evidence implicating C-type viruses in at least some human osteosarcomas (Zurcher et al, 1975;Pritchard et al, 1974;Morton and Malmgren, 1968). Assuming that human osteosarcomas are virally induced, the various types of cultures may reflect different modes of viral transformation.…”

Section: Skin-derived Cell Lines From Osteosarcoma Patienismentioning

confidence: 99%

The biology of human cells in tissue culture. I. Characterization of cells derived from osteogenic sarcomas

Smith

Owens

Hiller

et al. 1976

Intl Journal of Cancer

View full text Add to dashboard Cite

We have extensively characterized cell lines derived from six human osteosarcomas. The growth properties of these cultures were compared to those of fibroblastic cultures derived from skin of osteosarcoma patients and skin of bone-marrow of normal individuals. Each tumor-derived line showed some but not all of the abnormal growth properties usually associated with malignant transformation, indicating that tumor cells rather than normal stromal cells had in fact been cultured. The parameters measured included saturation density, cell morphology, growth pattern, growth on contact inhibited monolayers of normal fibroblastic or epithelial cells and tumorigenicity in immunosuppressed mice. Although the skin fibroblasts from the osteosarcoma patients appeared normal in vitro, they showed a greater ability to grow in immunosuppressed mice than did normalfibroblasts. This observation suggests that all fibroblasts of osteosarcoma patients may have an increased propensity for malignant transformation.

show abstract

Section: Skin-derived Cell Lines From Osteosarcoma Patienismentioning

confidence: 99%

The biology of human cells in tissue culture. I. Characterization of cells derived from osteogenic sarcomas

Smith

Owens

Hiller

et al. 1976

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…The possible origin of these antibodies is discussed. Evidence for the presence of oncornavirus particles (1-13) as well as viral antigens (14)(15)(16)(17)(18)(19)(20), RNA-dependent DNA polymerase (reverse transcriptase) (21)(22)(23)(24)(25), and nucleic acids (22,(26)(27)(28)(29) in normal and malignant human tissues has accumulated steadily over the past few years. Another approach to search for C type virus information in humans is to screen sera from healthy individuals and from patients for antibodies reacting with tumor virus antigens.…”

mentioning

confidence: 99%

Human antibodies reactive with purified envelope antigens of primate type C tumor viruses.

Kurth¹,

Mikschy²

1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Human sera from healthy individuals have been shown to react with viral antigens in preparations of de-tergent-disrupted C type viruses from monkeys. In this report, it is demonstrated that (populations of) these human antibodies react with highly purified envelope glycoproteins of the simian sarcoma virus-simian sarcoma-associated virus complex and the Friend leukemia virus complex. Several immunological parameters influencing human antibody binding to C type tumor virus antigens have been characterized. These parameters indicate that human antibodies tend to bind to what is probably a subset of the antigenic determinants on the virus envelope antigens and that different human sera recognize the same antigenic determinants on the virus envelope antigens tested. The possible origin of these antibodies is discussed. Evidence for the presence of oncornavirus particles (1-13) as well as viral antigens (14-20), RNA-dependent DNA polymerase (reverse transcriptase) (21-25), and nucleic acids (22,(26)(27)(28)(29) in normal and malignant human tissues has accumulated steadily over the past few years. Another approach to search for C type virus information in humans is to screen sera from healthy individuals and from patients for antibodies reacting with tumor virus antigens. The presence of such antibodies may indicate previous viral infection.In the absence of C type tumor virus strains of undisputable human origin, we made use of the observation that comparative analysis of C type animal viruses generally revealed close immunological and biochemical similarities between virus strains isolated from the same or evolutionary related species. It could, therefore, be assumed that the yet putative human C type viruses may share antigenic determinants and nucleic acid homologies with virus strains of higher primates-i.e., monkeys and apes. For this reason we have used structural proteins of C type viruses from monkeys to survey human sera for the presence of antibodies reacting with C type viral antigens.Our previous investigations revealed the presence of antibodies in the majority of human sera that react with antigens in preparations of C type primate viruses (30-3). These data are in agreement with related findings from some laboratories (34-36), but contradict reports from several others that were unable to demonstrate human antibodies reacting with RNA tumor virus antigens (37-39). The reasons for these discrepancies remain unclear at present, but they may have been caused by using different human sera, different immunological detection techniques, virus antigens prepared by different procedures, and different interpretation of the data (discussed in refs. 30 and 31).To resolve these discrepancies, both improvement of the immunological detection techniques and the use of viral antigens purified to homogeneity (40, 41) seemed imperative.This publication deals with the presence of antibodies inThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby...

show abstract

“…Rat antimurine leukemia virus (MuLV) serum used in the neutralization of focus formation was prepared in one of our laboratories (PB) and has been described elsewhere (Zurcher et al, 1975). The goat anti-SSV serum used was provided by Dr. Carolyn Bergholz, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Ill., USA, and was reported to have a neutralizing activity for SSV (50% inhibition of focus formation) at 1512.…”

Section: Antiserummentioning

confidence: 99%

Detection of human c‐type “helper” viruses in human leukemic bone marrow with murine sarcoma virus‐transformed human and rat non‐producer cells

Nooter

Bentvelzen

Zürcher

et al. 1977

Intl Journal of Cancer

View full text Add to dashboard Cite

Bone-marrow cells from two leukemic children were co-cultivated with the leukemic children A 7573. In early passages, C-type oncornaviruses were released as detected by extracellular reverse transcriptase assay. Co-cultivation of the infected canine cells with the non-producing cell lines R-970-5 (human) or K-NRK (rat) both transformed by Kirsten mouse sarcoma virus (MSV) yielded a new pseudotype of MSV that could transform rat embryo, rabbit SIRC and human kidney cells but not mouse embryo cells. The focur formation could be inhibited by an antiserum to the simian sarcoma virus but not by a serum directed against murine leukemia virus. A cell line derived from a focus of transformed cells became a highe virus is related to the simian sarcoma virus. It is concluded that the leukemic bone-marrow cells produce a C-type oncornavirus that can serve as a helper virus to the defective MSV.

show abstract

C-type virus antigens detected by immuno-fluorescence in human bone tumour cultures

Cited by 32 publications

References 19 publications

The biology of human cells in tissue culture. I. Characterization of cells derived from osteogenic sarcomas

The biology of human cells in tissue culture. I. Characterization of cells derived from osteogenic sarcomas

Human antibodies reactive with purified envelope antigens of primate type C tumor viruses.

Detection of human c‐type “helper” viruses in human leukemic bone marrow with murine sarcoma virus‐transformed human and rat non‐producer cells

Contact Info

Product

Resources

About