C1QBP inhibits proliferation of porcine circovirus type 2 by restricting nuclear import of the capsid protein

Ma, Xin; Lv, Changjie; Wang, Qianqian; Li, Chen; Wang, Peixin; Luo, Chen; Wu, Yifan; Wei, Tingting; Liu, Siying; Adam, Fathalrhman Eisa Addoma; Yang, Zhicong; Wang, Xinglong

doi:10.1007/s00705-020-04950-7

Cited by 5 publications

(5 citation statements)

References 35 publications

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“…ON180781) propagated in PAMs were used in this study. The virus titer in PAMs was assessed through an indirect fluorescent antibody assay (IFA) conducted as previously described ( Ma et al, 2021 ), with PRRSV-N mAb (monoclonal antibody, gifted by Professor Shuqi Xiao from NWAFU) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Abcam, Cambridge, United Kingdom) as primary and secondary antibody, respectively. The 50% tissue culture infective dose (TCID50) was calculated using the Reed-Muench method.…”

Section: Methodsmentioning

confidence: 99%

The transcriptional characteristics of NADC34-like PRRSV in porcine alveolar macrophages

Wang

Ma²,

Zhang³

et al. 2022

Front. Microbiol.

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The widespread and endemic circulation of porcine reproductive and respiratory syndrome virus (PRRSV) cause persistent financial losses to the swine industry worldwide. In 2017, NADC34-like PRRSV-2 emerged in northeastern China and spread rapidly. The dynamics analysis of immune perturbations associated with novel PRRSV lineage is still incomplete. This study performed a time-course transcriptome sequencing of NADC34-like PRRSV strain YC-2020-infected porcine alveolar macrophages (PAMs) and compared them with JXA1-infected PAMs. The results illustrated dramatic changes in the host’s differentially expressed genes (DEGs) presented at different timepoints after PRRSV infection, and the expression profile of YC-2020 group is distinct from that of JXA1 group. Functional enrichment analysis showed that the expression of many inflammatory cytokines was up-regulated following YC-2020 infection but at a significantly lower magnitude than JXA1 group, in line with the trends for most interferon-stimulated genes (ISGs) and their regulators. Meanwhile, numerous components of histocompatibility complex (MHC) class II and phagosome presented a stronger transcription suppression after the YC-2020 infection. All results imply that YC-2020 may induce milder inflammatory responses, weaker antiviral processes, and more severe disturbance of antigen processing and presentation compared with HP-PRRSV. Additionally, LAPTM4A, GLMP, and LITAF, which were selected from weighted gene co-expression network analysis (WGCNA), could significantly inhibit PRRSV proliferation. This study provides fundamental data for understanding the biological characteristics of NADC34-like PRRSV and new insights into PRRSV evolution and prevention.

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Section: Methodsmentioning

confidence: 99%

The transcriptional characteristics of NADC34-like PRRSV in porcine alveolar macrophages

Wang

Ma²,

Zhang³

et al. 2022

Front. Microbiol.

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“…Further studies showed that the arginine-rich N-terminal NLS motif of PCV Cap could directly interact with the serine-48 residue at the N-terminal oligomerization domain of NPM1 [ 59 , 60 , 72 , 73 ]. Moreover, the C1QBP is a crucial mitochondrial matrix protein located on the cell surface, nucleus, and cytoplasm [ 74 , 75 , 76 ]. Therefore, C1QBP can interact with the NLS of viral Cap, thus inhibiting PCV proliferation by restricting the nuclear import [ 74 , 76 ].…”

Section: Crosstalk Between Pcv and Hostmentioning

confidence: 99%

“…Moreover, the C1QBP is a crucial mitochondrial matrix protein located on the cell surface, nucleus, and cytoplasm [ 74 , 75 , 76 ]. Therefore, C1QBP can interact with the NLS of viral Cap, thus inhibiting PCV proliferation by restricting the nuclear import [ 74 , 76 ]. C1QBP was downregulated at the early stage of PCV infection [ 74 , 76 ].…”

Section: Crosstalk Between Pcv and Hostmentioning

confidence: 99%

“…Therefore, C1QBP can interact with the NLS of viral Cap, thus inhibiting PCV proliferation by restricting the nuclear import [ 74 , 76 ]. C1QBP was downregulated at the early stage of PCV infection [ 74 , 76 ]. Interestingly, C1QBP was also recruited into the nucleus by interacting with the viral Cap’s NLS (24RRR26) [ 75 ].…”

Section: Crosstalk Between Pcv and Hostmentioning

confidence: 99%

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Advances in Crosstalk between Porcine Circoviruses and Host

Niu

Chen

et al. 2022

Viruses

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Porcine circoviruses (PCVs), including PCV1 to PCV4, are non-enveloped DNA viruses with a diameter of about 20 nm, belonging to the genus Circovirus in the family Circoviridae. PCV2 is an important causative agent of porcine circovirus disease or porcine circovirus-associated disease (PCVD/PCVAD), which is highly prevalent in pigs and seriously affects the swine industry globally. Furthermore, PCV2 mainly causes subclinical symptoms and immunosuppression, and PCV3 and PCV4 were detected in healthy pigs, sick pigs, and other animals. Although the pathogenicity of PCV3 and PCV4 in the field is still controversial, the infection rates of PCV3 and PCV4 in pigs are increasing. Moreover, PCV3 and PCV4 rescued from infected clones were pathogenic in vivo. It is worth noting that the interaction between virus and host is crucial to the infection and pathogenicity of the virus. This review discusses the latest research progress on the molecular mechanism of PCVs–host interaction, which may provide a scientific basis for disease prevention and control.

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“…Notably, PCV2 infection promoted IFN-β production via activating the cGAS/STING signaling, while IFN-α, IFN-β, IFN-γ, concanavalin A (ConA), and IL-2 can enhance PCV2 replication [3][4][5][6][7][8][9][10][11]. On the other hand, intracellular host restriction factors, such as 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), complement component 1Q subcomponent-binding protein (C1QBP), and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein (14-3-3β/α, YWHAB), play important roles in resisting virus infection [1,2,[12][13][14]. Among the intracellular host factors, tripartite motif proteins (TRIMs) are a kind of newly discovered host protein involved in various cellular functions, including cell cycles, immunity, carcinogenesis, apoptosis, as well as virus infection [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Porcine TRIM21 Enhances Porcine Circovirus 2 Infection and Host Immune Responses, But Inhibits Apoptosis of PCV2-Infected Cells

Yang

Liu

Zhang

et al. 2022

Viruses

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Tripartite motif protein 21 (TRIM21) is an interferon-inducible E3 ligase, containing one RING finger domain, one B-box motif, one coiled-coil domain at the N-terminal, as well as one PRY domain and one SPRY domain at the C-terminal. TRIM21 is expressed in many tissues and plays an important role in systemic autoimmunity. However, TRIM21 plays different roles in different virus infections. In this study, we evaluate the relationship between porcine TRIM21 and PCV2 infection as well as host immune responses. We found that PCV2 infection modulated the expression of porcine TRIM21. TRIM21 can enhance interferons and proinflammatory factors and decrease cellular apoptosis in PCV2-infected cells. These results indicate that porcine TRIM21 plays a critical role in enhancing PCV2 infection, which is a promising target for controlling and developing the treatment of PCV2 infection.

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C1QBP inhibits proliferation of porcine circovirus type 2 by restricting nuclear import of the capsid protein

Cited by 5 publications

References 35 publications

The transcriptional characteristics of NADC34-like PRRSV in porcine alveolar macrophages

The transcriptional characteristics of NADC34-like PRRSV in porcine alveolar macrophages

Advances in Crosstalk between Porcine Circoviruses and Host

Porcine TRIM21 Enhances Porcine Circovirus 2 Infection and Host Immune Responses, But Inhibits Apoptosis of PCV2-Infected Cells

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