C24:0 avoids cold exposure-induced oxidative stress and fatty acid β-oxidation damage

Sun, Shouxiang; Cao, Xiaojuan; Gao, Jian

doi:10.1016/j.isci.2021.103409

Cited by 8 publications

(1 citation statement)

References 53 publications

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“…Nucleotide metabolites (such as 1-methylxanthine and 2’-deoxyinosine), organic acids and their derivatives (such as ethylmalonate and 4-acetamidobutyric acid), and several long-chain fatty acids (C > 20) (such as behenate and lignocerate) indicated that G. przewalskii might adapt to cold stress by altering energy metabolism. D. rerio might synthesize abundant C24:0 via elovl1 to promote mitochondrial β -oxidation and provide the required energy for resisting the cold environment [ 70 ]. In addition, lignocerate (C24:0) and the key gene elovl1a/b increased in the hepatopancreas, intestine, and muscle.…”

Section: Discussionmentioning

confidence: 99%

Physiological, metabolomic, and transcriptomic reveal metabolic pathway alterations in Gymnocypris przewalskii due to cold exposure

Liu,

Tian,

et al. 2023

BMC Genomics

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Teleost fish have evolved various adaptations that allow them to tolerate cold water conditions. However, the underlying mechanism of this adaptation is poorly understood in Tibetan Plateau fish. RNA-seq combined with liquid chromatography‒mass spectrometry (LC‒MS/MS) metabolomics was used to investigate the physiological responses of a Tibetan Plateau-specific teleost, Gymnocypris przewalskii, under cold conditions. The 8-month G. przewalskii juvenile fish were exposed to cold (4 ℃, cold acclimation, CA) and warm (17 ℃, normal temperature, NT) temperature water for 15 days. Then, the transcript profiles of eight tissues, including the brain, gill, heart, intestine, hepatopancreas, kidney, muscle, and skin, were evaluated by transcriptome sequencing. The metabolites of the intestine, hepatopancreas, and muscle were identified by LC‒MS/MS. A total of 5,745 differentially expressed genes (DEGs) were obtained in the CA group. The key DEGs were annotated using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. The DEGs from the eight tissues were significantly enriched in spliceosome pathways, indicating that activated alternative splicing is a critical biological process that occurs in the tissues to help fish cope with cold stress. Additionally, 82, 97, and 66 differentially expressed metabolites were identified in the intestine, hepatopancreas, and muscle, respectively. Glutathione metabolism was the only overlapping significant pathway between the transcriptome and metabolome analyses in these three tissues, indicating that an activated antioxidative process was triggered during cold stress. In combination with the multitissue transcriptome and metabolome, we established a physiology-gene‒metabolite interaction network related to energy metabolism during cold stress and found that gluconeogenesis and long-chain fatty acid metabolism played critical roles in glucose homeostasis and energy supply.

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Section: Discussionmentioning

confidence: 99%