C30 Stationary phases for the analysis of food by liquid chromatography

Sander, Lane C.; Sharpless, Katherine E.; Pursch, Matthias

doi:10.1016/s0021-9673(00)00121-7

Cited by 171 publications

(79 citation statements)

References 61 publications

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“…In reverse HPLC, the retention time of carotenoids goes from greater to lesser polarity. It was shown that the separation of the polar carotenoids is influenced by the presence of water whereas non-polar carotenoids are relatively insensitive to this parameter (Sander et al 2000). In relation to these chromatographic characteristics, we used an appropriate mobile phase, with an adjusted quaternary gradient to enhance the separation and the selectivity of most polar carotenoids, such as neoxanthin, violaxanthin, antheraxanthin, epilutein.…”

Section: Discussionmentioning

confidence: 99%

Development of a method to determine carotenoid composition of fresh forages

Cardinault¹,

Lyan²,

Doreau³

et al. 2008

Can. J. Plant Sci.

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Development of a method to determine carotenoid composition of fresh forages. Can. J. Plant Sci. 88: 1057Á1064. Due to the limited interest in carotenoids in ruminant diets until recently, analyses of forages are often incomplete, focusing mainly on b-carotene and lutein. Carotenoid composition of green forage from middle mountain meadow was analyzed by HPLC after extraction and elimination of chlorophylls by mild saponification. This method of analysis uses two C 18 columns in series with a quaternary gradient system. Our method allowed, for the first time, the identification and quantification of several xanthophylls other than lutein (i.e., violaxanthin, antheraxanthin, epilutein) in chlorophyll-free extracts from carotenoidrich forage. The intra-day (3.5Á7.5%) and inter-day (1.2Á3.5%) coefficients of variation are suitable for routine determination of carotenoids in green forage. This method could also be used in metabolic studies of these micronutrients in ruminants.Key words: Xanthophylls, carotenoids, fresh forage, HPLC Cardinault, N., Lyan, B., Doreau, M., Chauveau, B., Rock, E. et Grolier, P. 2008. De´veloppement d'une me´thode afin de de´terminer la composition en carote´noı¨des de fourrages frais. Can. J. Plant Sci. 88: 1057Á1064. En raison de l'inte´reˆt limitej usqu'a`maintenant pour les carote´noı¨des dans les re´gimes des ruminants, les analyses sont souvent incomple`tes et se focalisant principalement sur le b-carote`ne et la lute´ine. La composition en carote´noı¨des d'un fourrage vert d'une prairie de moyenne montagne a e´te´analyse´e par CLHP apre`s extraction et e´limination des chlorophylles par une saponification mode´re´e. Cette me´thode d'analyse utilise deux colonnes C18 en se´rie avec un syste`me de gradient quaternaire. Notre me´thode a permis pour la premie`re fois d'identifier et de quantifier plusieurs autres xanthophylles (violaxanthine, antheraxanthine, epiluteine) que la luteine dans un extrait de fourrage riche en carote´noı¨des de´pourvu de chlorophylles Les coefficients de variations intra-jour (3,5%-7,5%) et inter-jour (1,2%-3,5%) sont adapte´s pour une analyse courante des carote´noı¨des dans les fourrages verts. Cette me´thode pourrait aussi eˆtre utilise´e dans des e´tudes me´taboliques sur ces micronutriments chez les ruminants.

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Section: Discussionmentioning

confidence: 99%

Development of a method to determine carotenoid composition of fresh forages

Cardinault¹,

Lyan²,

Doreau³

et al. 2008

Can. J. Plant Sci.

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“…Fröhlich et al [218] also used a C30 column to distinguish between lycopene isomers. Extensive reviews of the development of C30 stationary phases for carotenoid analysis can be found elsewhere [219].…”

Section: Chromatographic Methodsmentioning

confidence: 99%

New Developments in Lycopene Analysis by Spectroscopic and Chromatographic Techniques, Accompanied With Mathematical Modelling

Cáceres¹,

Cámara²,

Manzoor³

et al. 2015

Acta Hortic.

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“…However, due to the high backpressure of C30 columns, analysis time tend to be rather long [4]. These methods also often use hazardous and nonenvironmentally friendly mobile phases such as acetonitrile, chloroform or methyl tert-butyl ether [3,5,6].…”

Section: Introductionmentioning

confidence: 99%

Determination of carotenoids in microalgae using supercritical fluid extraction and chromatography

Abrahamsson

Rodríguez‐Meizoso

Turner

2012

Journal of Chromatography A

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A method was developed based on supercritical fluid chromatography for quantitative determination of carotenoids in extracts of Scenedesmus sp. By utilizing the low backpressure in supercritical fluid chromatography, a C18 column and a 2-ethyl pyridine column were coupled in series. It was concluded that even minor changes in temperature had a substantial effect on selectivity. A standard mixture of 8 carotenoids and microalgae extracts obtained through supercritical fluid extraction with and without 10% ethanol as a co-solvent were successfully separated. All of the carotenoids were separated within 10 minutes, while the total analysis time was 20 minutes. The method was validated and the carotenoids of microalgae extracts were quantified. Furthermore, the method should be seen as a more rapid and environmentally sustainable alternative to traditional high-performance liquid chromatography methods utilizing organic solvents.

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C30 Stationary phases for the analysis of food by liquid chromatography

Cited by 171 publications

References 61 publications

Development of a method to determine carotenoid composition of fresh forages

Development of a method to determine carotenoid composition of fresh forages

New Developments in Lycopene Analysis by Spectroscopic and Chromatographic Techniques, Accompanied With Mathematical Modelling

Determination of carotenoids in microalgae using supercritical fluid extraction and chromatography

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