C4d staining in allograft biopsies

Kulkarni, PG; Ms, Uppin; Ak, Prayaga; Das, Upasak; Kv, Dakshinamurthy

doi:10.4103/0971-4065.97155

Cited by 2 publications

(2 citation statements)

References 10 publications

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“…Our experience with allograft biopsies has been published earlier. [ 10 11 ] The standard triple immunosupression is followed at our centre that includes steroid based therapy, calcineurine inhibitors (Cyclosporine or Tacrolimus) along with azathioprine or mycophenolate mofetil. All biopsies from January 2013 to October 2014 were reviewed.…”

Section: Methodsmentioning

confidence: 99%

Expanding the antibody-mediated component of plasma cell-rich acute rejection: A case series

Ms¹,

Swarnalatha²,

Taduri³

et al. 2016

Indian J Nephrol

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Renal allograft rejection is mediated by T-cells (T-cell mediated rejection) or by donor-specific antibodies (DSAs) (antibody mediated rejection, ABMR). Plasma cell-rich acute rejection (PCAR) is a unique entity due to its peculiar morphology and poor prognostic behavior. All allograft biopsies done at our center from January 2013 to October 2014 were reviewed, and seven were identified with a diagnosis of PCAR with antibody mediated rejection (ABMR). The allograft biopsies were classified as per the Banff 2007 schema. Immunohistochemistry with C4d, SV 40, CD3, CD20, CD138, kappa and lambda light chain was performed. Total 210 allograft biopsies were performed in the study period of which seven biopsies (3.3%) were diagnosed as PCAR with ABMR. All these were late ABMRs (more than 6 months) with median posttransplant duration of 17 months. The allograft biopsy showed features of PCAR along with glomerulitis, peritubular capillaritis, and positive C4d. DSA was positive in six patients. All the patients were treated with standard therapeutic measures of acute cellular rejection (ACR) and ABMR including steroids, plasma exchange, rituximab and intravenous immunoglobulins. All the patients had persistent graft dysfunction or graft loss on follow-up.

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Section: Methodsmentioning

confidence: 99%

Expanding the antibody-mediated component of plasma cell-rich acute rejection: A case series

Ms¹,

Swarnalatha²,

Taduri³

et al. 2016

Indian J Nephrol

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“…ABMR is dominated by endothelial damage in microcirculation [ 7 , 8 ]. Microcirculation inflammation, including glomerulitis and peritubular capillaritis (PTCitis), has been recognized as a cardinal feature in the diagnosis of ABMR [ 9 , 10 ]. Peritubular capillary dilation is another important side of microcirculation changes, and although it has been noticed in the ABMR for years [ 2 ], it is far from being clearly demonstrated and its pathogenesis remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Capillary Dilation and Rarefaction Are Correlated with Intracapillary Inflammation in Antibody-Mediated Rejection

Sun

Zhang

et al. 2014

Journal of Immunology Research

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Antibody-mediated rejection (ABMR) remains one of the major causes of graft loss after renal transplantation. It is dominated by endothelial damage in microcirculation. Clarifying the mechanism of microcirculating damage is obviously a key step to understand the pathogenesis of ABMR. Here we characterized capillary variation in ABMR and its possible mechanisms. Compared with T cell-mediated rejection and stable grafts, there was a significant dilation and rarefaction in peritubular capillaries (PTCs) of the ABMR group; Image-Pro Plus revealed a significantly larger intra-PTC area. Interestingly, the dilation of PTCs was strongly correlated with the intra-PTC cell counting. Moreover, peritubular capillary inflammation is correlated with in situ T-bet expression, and there was a good correlation between the intra-PTC expression of T-bet and the PTC diameter. HIF-1α up-regulation could be observed in ABMR but it was not necessary for capillary dilation. In general, ABMR is characterized with early capillary dilation and rarefaction; our data confirmed that the dilation is strongly correlated with intracapillary inflammation, which in turn is correlated with in situ T-bet expression. T-bet plays an important role in the development of microcirculating injury, and thus it is a potential target for the treatment of ABMR.

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C4d staining in allograft biopsies

Cited by 2 publications

References 10 publications

Expanding the antibody-mediated component of plasma cell-rich acute rejection: A case series

Expanding the antibody-mediated component of plasma cell-rich acute rejection: A case series

Capillary Dilation and Rarefaction Are Correlated with Intracapillary Inflammation in Antibody-Mediated Rejection

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