C6-aldehyde formation from linolenic acid in fruit cells cultured in vitro

Abstract

Heterotrophic plant cells of three apple cultivars, two pear cultivars, strawberry, and tomato were cultured in vitro. Cell homogenates in the presence of linolenic acid formed n-hexanal, (Z)-3-hexenal, (E)-2-hexenal, and, in one apple cultivar, (Z)-3-hexenol and its acetate. Homogenates of photomixotropic apple cv. Goldparmfine cells contained eight times more (E)-2-hexenal than homogenates of non-green cells.

Development of a Biocatalytic Process for the Production of C6-Aldehydes from Vegetable Oils by Soybean Lipoxygenase and Recombinant Hydroperoxide Lyase

“…The subsequent conversion of hydroperoxy fatty acids by hydroperoxide lyase, however, caused problems. HPL in plant extracts is very unstable, and molar conversion rates were often <1% ( − ). Screening of different vegetables resulted in yields of 36% for hexanal and 24% for (2 E )-hexenal ().…”

La lipoxigenasa en el reino vegetal, asignadas. II. Funciones fisiológicas

“…Los aldehidos producidos por la hidroperóxido liasa pueden ser reducidos a los correspondientes alcoholes en algunos vegetales. Estos productos con función aldehido y alcohol son componentes habituales del sabor y olor de hojas y frutos (Hamilton-Kemp y Andersen; 1986; Kumar y Motto, 1986;Berger et al, 1987). El papel fisiológico de los aldehidos también es bastante enigmático.…”

Modeling hexanal production in oxido-reducing conditions by the yeast Yarrowia lipolytica

“…The cultures of different fruit cells were used to produce cis-3-, trans-2-hexenal [10,11] but the yield of recovery was low (<0.18 mg/kg). A screening of many vegetables and fruits was performed [12] where a maximum of 370 mg of hexanal/kg of reaction medium was obtained with a homogenate of green bell pepper and linoleic acid as substrate; however, the conversion rate was low (<16%).…”

“…The literature reported several processes based on the use of plant homogenate to produce natural hexanal, 3(Z)-or 2(E)-hexenals and 3(Z)-or 2(E)-hexenols from either linoleic or a-linolenic acids as substrates [6][7][8][9][10][11][12]. The cultures of different fruit cells were used to produce cis-3-, trans-2-hexenal [10,11] but the yield of recovery was low (<0.18 mg/kg).…”