1986
DOI: 10.1016/0014-5793(86)81152-8
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Ca2+‐dependent inactivation of acetylcholine receptors by an endogenous transglutaminase

Abstract: The nicotinic acetylcholine receptor (nAChR) from Torpedo californica and T. marmorata electric tissue polymerises irreversibly when DTE and CaZ+ are added to receptor-rich membranes. The polymerisation is time-dependent and complete within 3 h at 30°C. It can be completely prevented by EGTA or the transglutaminase inhibitor cystamine. Transglutaminase activity can also be monitored with the exogenous substrates [3H]putrescine and dimethylcasein. This assay can also be inhibited by EGTA or cystamine.Nicotinic … Show more

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Cited by 14 publications
(11 citation statements)
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“…On normal skin TGase 2 was mainly localized on the dermis and the basement membrane. Incubation of sections with EGTA prohibited the incorporation of the biotinylated peptides, which is in accord with previous observations where it was mentioned that both EGTA and EDTA, Ca +2 chelating agents, reduce the availability of calcium ions and consequently lower the activity of TGase 2, 5, 18, 63, 64…”
Section: Discussionsupporting
confidence: 90%
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“…On normal skin TGase 2 was mainly localized on the dermis and the basement membrane. Incubation of sections with EGTA prohibited the incorporation of the biotinylated peptides, which is in accord with previous observations where it was mentioned that both EGTA and EDTA, Ca +2 chelating agents, reduce the availability of calcium ions and consequently lower the activity of TGase 2, 5, 18, 63, 64…”
Section: Discussionsupporting
confidence: 90%
“…A number of artificial TGase inhibitors have been introduced;23 however, the intrinsically high reactivity of alkylating agents toward thiol groups limits their therapeutic utility; covalent inhibitors like alkyl isocyanates lack specificity; competitive inhibitors such as methylamine and dansyl cadaverine (primary amines) are active only at high concentrations, inhibit cell attachment and have potent toxic effects 65–67. Cystamine has beneficial effects over a number of diseases and has been used to block the active site thiol group by forming mixed disulfide bonds 5, 29, 68–70. In this study, di‐BOC cystamine had little if any effect on endogenous TGase activity, whilst cystamine dihydrochloride successfully inhibited TGase only at high concentrations as has been shown before 26.…”
Section: Discussionmentioning
confidence: 99%
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“…Transglutaminase at the neuromuscular junction may contribute to the stability of the postsynaptic cholinergic system. Transglutaminase activity copurifies with nicotinic acetylcholine receptor (nAChR) subunits, which undergo calcium-dependent cross-linking in vitro (Hucho and Bandini, 1986). The collagen-tailed form of acetylcholinesterase (AChE) is a substrate for transglutaminases (Hand and Haynes, 1992).…”
mentioning
confidence: 99%
“…For example, the electroplax is now cooled in liquid nitrogen and pulverized in a mortar and pestle as described by Burden et al (1983) prior to further homogenization, a procedure that ensures good penetration of buffers containing EGTA and EDTA at the moment of thawing. It is clear that membranes enriched in the nicotinic cholinergic receptor and protected from autooxidation and other calcium-stimulated activities [proteases (Damle and Karlin, 1978;Schiebler and Hucho, 1978) and transglutaminases (Hucho and Bandini, 1986)J may be transformed into an alkali-resistant state whether the sulfhydryl alkylation is performed ab initio or after storage at -56°C for weeks.…”
Section: Resultsmentioning
confidence: 99%