Ca++ Uptake by Rat Kidney Mitochondria and Its Dependence on Respiration and Phosphorylation

Vasington, Frank D.; Murphy, Jerome V.

doi:10.1016/s0021-9258(19)73805-8

Cited by 571 publications

(60 citation statements)

References 38 publications

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“…during fixation and dehydration . Most of the intrinsic mitochondrial phosphorus is in the form of phospholipids (Vasington and Murphy, 1962 ;Chappell et al ., 1963) susceptible to extraction during preparative procedures . Mitochondrial P is detectable by electron probe X-ray microanalysis of frozen sections (Weavers, 1972) .…”

Section: Electron Probe X-ray Microanalysismentioning

confidence: 99%

Electron Microscopy and Electron Probe Analysis of Mitochondrial Cation Accumulation in Smooth Muscle

Somlyo¹,

Devine²,

Peters³

et al. 1974

The Journal of Cell Biology

130

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The contractile responses to barium and the ultrastructure and ionic composition of mitochondria were studied in vascular smooth muscle . In normal rabbit portal anterior mesenteric vein (PAMV) and main pulmonary artery (MPA) smooth muscle mitochondria were frequently associated with the surface vesicles . The Electron probe X-ray microanalysis also showed a highly significant (P < 0 .001) correlation of P with mitochondrial Ba, in an estimated elemental ratio of approximately 3 Ba/4 P . Mitochondrial granules were still prominent after block staining of the osmium-fixed, Ba-loaded PAMV, but electron probe microanalysis showed no Ba, but only U, emissions . Tissues incubated with strontium had electron-opaque mitochondrial granules and deposits in the sarcoplasmic reticulum . X-ray microanalysis of mitochondria containing granules showed the presence of characteristic Sr and Ca emissions . The presence of Sr was similarly verified in the sarcoplasmic reticulum . These findings indicate the energy dependent uptake of divalent cations, in association with phosphate, by mitochondria in vascular smooth muscle in situ and the possibility that mitochondria may contribute to the regulation of intracellular divalent cation levels in smooth muscle .

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Section: Electron Probe X-ray Microanalysismentioning

confidence: 99%

Electron Microscopy and Electron Probe Analysis of Mitochondrial Cation Accumulation in Smooth Muscle

Somlyo¹,

Devine²,

Peters³

et al. 1974

The Journal of Cell Biology

130

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“…For decades, it has been known that calcium ions can enter mitochondria through a highly selective uniporter channel, driven by the electrochemical gradient across the inner mitochondrial membrane (IMM) (Carafoli and Lehninger, 1971;Deluca and Engstrom, 1961;Kirichok et al, 2004;Vasington and Murphy, 1962). Numerous studies have confirmed that calcium uptake in isolated mitochondria leads to transient dissipation of the membrane potential and sustained enhancement of NAD(P)H autofluorescence, oxygen consumption, and ATP phosphorylation (Territo et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Exploring the In Vivo Role of the Mitochondrial Calcium Uniporter in Brown Fat Bioenergetics

et al. 2019

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“…Studies of metal distribution in isolated cell fractions have generally implicated mitochondria as one site of divalent ion accumulation. Mitochondria, when isolated, are known to contain relatively large amounts of calcium and magnesium, and to be able to accumulate these ions, strontium, and manganese in vitro (Cleland, 1953;Slater and Cleland, 1953;Bartley and Amoore, 1958;De Luca and Engstrom, 1961;Mraz, 1962;Brierley et al, 1962;Vasington and Murphy, 1962;Chappell et al, 1962;Lehninger et al, 1963;Saris, 1963). Manganese administered intravenously to rats has been found to reach a high level in the mitochondrial fraction of liver (Maynard and Cotzias, 1955), and the unusually high calcium content of livers of rats poisoned with carbon tetrachloride has been shown by Thiers et al (1960) to be due to a twentyfold increase in mitochondrial calcium.…”

Section: Introductionmentioning

confidence: 99%

Electron Microscopic Observations on the Accumulation of Divalent Cations in Intramitochondrial Granules

Peachey

1964

The Journal of Cell Biology

286

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Electron microscopic evidence is presented, from mitochondria in whole cells of toad urinary bladder and from isolated rat kidney mitochondria, indicating that the divalent cations calcium, strontium, and barium are accumulated in granules localized in the mitochondrial matrix. This accumulation occurs under conditions in which divalent ions are present in the medium bathing either whole cells or isolated mitochondria. The evidence indicates that the divalent ions are deposited on, or in a pre-existing granule, possibly in exchange for other ions. It suggests a possible role of the intramitochondrial granules in the regulation of the internal ionic environment of the mitochondrion. Certain biochemical and physiological implications of this phenomenon are discussed.

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Ca++ Uptake by Rat Kidney Mitochondria and Its Dependence on Respiration and Phosphorylation

Cited by 571 publications

References 38 publications

Electron Microscopy and Electron Probe Analysis of Mitochondrial Cation Accumulation in Smooth Muscle

Electron Microscopy and Electron Probe Analysis of Mitochondrial Cation Accumulation in Smooth Muscle

Exploring the In Vivo Role of the Mitochondrial Calcium Uniporter in Brown Fat Bioenergetics

Electron Microscopic Observations on the Accumulation of Divalent Cations in Intramitochondrial Granules

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