CA125 and UQCRFS1 FISH studies of ovarian carcinoma

Kaneko, Saori; Gerasimova, Tsilya; Smith, Shelby; Lloyd, Kenneth O.; Suzumori, Kaoru; Young, S. R.

doi:10.1016/s0090-8258(03)00144-6

Cited by 27 publications

(16 citation statements)

References 21 publications

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“…[35] and 13.6% ovarian cases reported by Kaneko et al . [34]. Data presented in this study suggest a correlation between the mtOXPHOS defects with the severity of breast cancer in that the aggressive metastatic breast cancer cell line, MDA-MB-231, contained the most aberrations in mtOXPHOS activity.…”

Section: Discussionmentioning

confidence: 56%

Impaired OXPHOS Complex III in Breast Cancer

et al. 2011

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We measured the mitochondrial oxidative phosphorylation (mtOXPHOS) activities of all five complexes and determined the activity and gene expression in detail of the Complex III subunits in human breast cancer cell lines and primary tumors. Our analysis revealed dramatic differences in activity of complex III between normal and aggressive metastatic breast cancer cell lines. Determination of Complex III subunit gene expression identified over expression and co-regulation of UQCRFS1 (encoding RISP protein) and UQCRH (encoding Hinge protein) in 6 out of 9 human breast tumors. Analyses of UQCRFS1/RISP expression in additional matched normal and breast tumors demonstrated an over expression in 14 out of 40 (35%) breast tumors. UQCRFS1/RISP knockdown in breast tumor cell line led to decreased mitochondrial membrane potential as well as a decrease in matrigel invasion. Furthermore, reduced matrigel invasion was mediated by reduced ROS levels coinciding with decreased expression of NADPH oxidase 2, 3, 4 and 5 involved in ROS production. These studies provide direct evidence for contribution of impaired mtOXPHOS Complex III to breast tumorigenesis.

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Section: Discussionmentioning

confidence: 56%

Impaired OXPHOS Complex III in Breast Cancer

et al. 2011

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“…A gene thought to have an important role in ovarian cancer is MUC16 (mucin 16) gene that encodes the tumor marker CA125 [5][6][7]. This gene is located on chromosome 19p13.2 [8] and is coded by sequences present within approximately 179 kb of genomic DNA. As per the present available information, there is a discrepancy regarding the total number of exons present in MUC16 genomic DNA.…”

Section: Introductionmentioning

confidence: 99%

Polymorphisms in the MUC16 Gene: Potential Implication in Epithelial Ovarian Cancer

Bouanene

Kacem

Fatma

et al. 2010

Pathol. Oncol. Res.

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MUC16 plays an important role in epithelial ovarian cancer. In this paper, we studied the association between two tags SNPs of MUC16 and the risk of epithelial ovarian cancer. We aimed also to test the association between these tags SNPs and elevated level of the protein CA125. We analyzed a collection of 117 cases. Forty-one samples of patients with epithelial ovarian cancer and 76 samples from Tunisian volunteers were genotyped for two synonymous coding tags SNPs of the MUC16 gene (rs1596797, A/C and rs2547065, C/G) using polymerase chain reaction and sequencing. For the rs1596797 SNP, there was no significant difference in genotype distribution, a rare variation observed in only one patient. For the polymorphism rs2547065, mean CA125 levels were 24 and 78 UI/ml in patients with GG and GC genotypes versus 230 UI/ml in patients with CC genotype (P = 0.36). Compared to the C/C genotype, the 'G' allele (C/G+G/G genotypes) did not significantly modified the risk of developing epithelial ovarian cancer (OR = 0.43; 95% CI). As for the polymorphism rs1596797, compared to the C/C genotype, the 'A' allele (C/A+A/A genotypes) did not significantly modified the risk of developing epithelial ovarian cancer (OR = 881.7; 95% CI). MUC16 gene polymorphisms selected in this study are neither involved in genetic predisposition to epithelial ovarian cancer nor associated with CA125 level.

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“…This complex passes electrons from reduced ubiquinol to cytochrome c in the process of ATP synthesis. Recent studies report that the expression of Uqcrfs1 is associated with a malignant condition (Kaneko et al 2003). It is known that Cd produces excessive oxidative stresses in the cell possibly by generating free radicals and ROS to disturb the mitochondrial function (Fiskum et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Differential protein profile in zebrafish (Danio rerio) brain under the joint exposure of methyl parathion and cadmium

Ling

Huang

2012

Environ Sci Pollut Res

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As different chemicals, methyl parathion (MP) and cadmium (Cd) can induce neurotoxicity on the brain of aquatic ecosystems. This study aims to explore the differential expression proteins in the brain induced by their joint stress and their joint effects, which are poorly reported, and devotes finding novel biomarkers for monitoring their contamination in water and assessing their neurological effects. The bioaccumulation of MP and Cd in tissues after 96 h of exposure was first analyzed by GC and inductively coupled plasma-MS to provide insights into the interaction. Protein profile changes in the brains of the zebrafish (Danio rerio) exposed to MP and Cd were further investigated using the proteomic approach. The correlation of gene expression on the transcription level of mRNA and the translation level of protein was examined by real-time quantitative PCR and Western blotting analysis. It showed that Cd and MP have an interaction on their bioaccumulation, which suggests that their joint effect over 96 h might be antagonistic. Proteomics revealed that 22 protein spots changed their expression levels under stress, of which 16 proteins were identified using MS. These proteins were involved in oxidation/reduction, metabolism, energy production, receptor activity, and cytoskeleton assembly. Among them, five proteins with a remarkable abundance change are significantly suggested to play important roles in the joint effect. This work demonstrates that there exists an interaction between MP and Cd toxicities, which may aid in our understanding of the mechanism of neurotoxicity induced by joint stress. The results may also provide the possibility of the establishment of candidate biomarkers for monitoring MP and Cd contamination in water.

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CA125 and UQCRFS1 FISH studies of ovarian carcinoma

Cited by 27 publications

References 21 publications

Impaired OXPHOS Complex III in Breast Cancer

Impaired OXPHOS Complex III in Breast Cancer

Polymorphisms in the MUC16 Gene: Potential Implication in Epithelial Ovarian Cancer

Differential protein profile in zebrafish (Danio rerio) brain under the joint exposure of methyl parathion and cadmium

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